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Development of advanced high-speed AFM and analysis of proteins' action mechanism

Planned Research

Project AreaNovel measurement techniques for visualizing 'live' protein molecules at work
Project/Area Number 26119003
Research Category

Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

Allocation TypeSingle-year Grants
Review Section Complex systems
Research InstitutionKanazawa University

Principal Investigator

Ando Toshio  金沢大学, ナノ生命科学研究所, 特任教授 (50184320)

Co-Investigator(Renkei-kenkyūsha) UCHIHASHI Takayuki  名古屋大学, 理学研究科, 教授 (30326300)
KONNO Hiroki  金沢大学, ナノ生命科学研究所, 准教授 (80419267)
NAKAYAMA Takahiro  金沢大学, ナノ生命科学研究所, 准教授 (00532821)
WATANABE Shinji  金沢大学, ナノ生命科学研究所, 助教 (70455864)
Project Period (FY) 2014-07-10 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥140,920,000 (Direct Cost: ¥108,400,000、Indirect Cost: ¥32,520,000)
Fiscal Year 2018: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2017: ¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2016: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2015: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2014: ¥59,150,000 (Direct Cost: ¥45,500,000、Indirect Cost: ¥13,650,000)
Keywords高速AFM / 走査型プローブ顕微鏡 / 蛋白質 / バイオイメージング / ダイナミクス / タンパク質
Outline of Final Research Achievements

The aim of this study is largely classified into two: (1) Extensively exploring a new research field of “dynamic structural biology” that seeks to film protein molecules in dynamic action with high-speed AFM, not only by conducting the study by myself but also by collaborating with researchers of the project group as well as external researchers, and (2) enabling observation of new molecular phenomena that have been impossible with the current high-speed AFM, by advancing the capability of high-speed AFM. For the aim (1), our instruments were opened to these researchers. Through many collaborations, we succeeded in observing a variety of proteins, providing mechanistic insights into their molecular processes. For the aim (2), we succeeded in developing high-speed AFM combined with optical tweezers and super-resolution fluorescence microscopy based on high-speed AFM. The actual application studies of these new instruments are subjects to be done in the future.

Academic Significance and Societal Importance of the Research Achievements

タンパク質分子は生命の機能素子であり、その機能する仕組の解明は生命現象及び疾病の理解に必須である。代表者が開発した高速AFMは機能中のタンパク質分子を直接観察することを可能にし、機能メカニズムの理解を促進する。だが、多くの対象を観察するには、多くの研究者との共同研究が必要であった。また、現在の高速AFMでさえ観察できない現象があり、装置の高度化は必須であった。本研究の実施は、多くの多様なタンパク質系の理解を可能にしただけでなく、この顕微鏡を利用できる人材の育成にも繋がった。高速AFMの高度化は新規観察を可能にした。その本格的応用は今後の課題だが、更なる生命現象の理解に貢献するものと期待される。

Report

(6 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • 2015 Annual Research Report
  • 2014 Annual Research Report

Research Products

(120 results)

All 2020 2019 2018 2017 2016 2015 2014 Other

All Int'l Joint Research (10 results) Journal Article (33 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 33 results,  Open Access: 18 results,  Acknowledgement Compliant: 5 results) Presentation (59 results) (of which Int'l Joint Research: 28 results,  Invited: 48 results) Book (1 results) Remarks (15 results) Funded Workshop (2 results)

  • [Int'l Joint Research] University of Michigan/Texas A&M University/Vanderbilt University(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Paris Diderot(フランス)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] KU Leuven(ベルギー)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Lawrence Livermore National Laboratory/University of Michigan(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] KU Leuven-University of Leuven(Belgium)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Gdansk University of Technology(Poland)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Shanghai Institute of Applied Physics(China)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Aix-Marseille Universiy/CNRS, AFMB UMR 7257(France)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] State University of Milano-Bicocca(Italy)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] Colorado State University/University of California, Davis/Vanderbilt University(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies2020

    • Author(s)
      Takayuki Umakoshi, Shingo Fukuda, Ryota Iino, Takayuki Uchihashi, Toshio Ando*
    • Journal Title

      Biochimica et Biophysica acta. General subjects

      Volume: 印刷中 Pages: 129325-129325

    • DOI

      10.1016/j.bbagen.2019.03.011

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct Visualization of Avian Influenza H5N1 Hemagglutinin Precursor and its Conformational Change by High-Speed Atomic Force Microscopy2020

    • Author(s)
      Lim SK, Mohamed MS, Wang H, Hartono, Hazawa M, Kobayashi A, Voon DC, Kodera N, Ando T and Wong RW
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - General Subjects

      Volume: 印刷中 Pages: 129313-129313

    • DOI

      10.1016/j.bbagen.2019.02.015

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural insights into the substrate specificity switch mechanism of the type III protein export apparatus2019

    • Author(s)
      Y., Y. Ogawa, M. Kinoshita, N. Terahara, M. Shimada, N. Kodera, T. Ando, K. Namba, A. Kitao, K. Imada, T. Minamino
    • Journal Title

      Sttucture

      Volume: 印刷中 Pages: 965-976

    • DOI

      10.1016/j.str.2019.03.017

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cationic polymethacrylate-copolymer acts as an agonist for β-amyloid and antagonist for amylin fibrillation2019

    • Author(s)
      B. R. Sahoo, T. Genjo, T. Watanabe-Nakayama, A. K. Stoddard, T. Ando, K. Yasuhara, C. A. Fierke, A. Ramamoorthy
    • Journal Title

      Chemical Science

      Volume: 10 Pages: 3976-3986

    • DOI

      10.1039/c8sc05771k

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Inner lumen proteins stabilize doublet microtubules in cilia and flagella2019

    • Author(s)
      Owa M, Uchihashi T, Yanagisawa H, Yamano T, Iguchi H, Fukuzawa H, Wakabayashi K, Ando T, Kikkawa M
    • Journal Title

      Nat. Commun.

      Volume: 10 Pages: 1143-1143

    • DOI

      10.1038/s41467-019-09051-x

    • NAID

      120006591370

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Metastable asymmetrical structure of shaftless V1 motor2019

    • Author(s)
      S. Maruyama, K. Suzuki, M. Imamura, H. Sasaki, H. Matsunami, K. Mizutani, Y. Saito, F. L. Imai, Y. Ishizuka-Katsura, T. Kimura-Someya, M. Shirouzu, T. Uchihashi, T. Ando, I. Yamato, T. Murata
    • Journal Title

      Science Advances

      Volume: 5

    • DOI

      10.1126/sciadv.aau8149

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure of the UHRF1 Tandem Tudor Domain Bound to a Methylated Non-histone Protein, LIG1, Reveals Rules for Binding and Regulation2019

    • Author(s)
      Kori, S. Ferry, L. Matano, S. Jimenji, T. Kodera, N. Tsusaka, T. Matsumura, R. Oda, T. Sato, M. Dohmae, N. Ando, T. Shinkai, Y. Defossez, P. A. Arita, K.
    • Journal Title

      Structure

      Volume: 27 Pages: 1-12

    • DOI

      10.1016/j.str.2018.11.012

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The induction of RANKL molecule clustering could stimulate early osteoblast differentiation2019

    • Author(s)
      Sone E., Noshiro D., Ikebuchi Y., Nakagawa M., Khan M., Tamura Y., Ikeda M., Oki M., Murali R., Fujimori T., Yoda T., Honma M., Suzuki H., Ando T., Aoki K.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 509 Pages: 435-440

    • DOI

      10.1016/j.bbrc.2018.12.093

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Single-Unit Imaging of Membrane Protein-Embedded Nanodiscs from Two Oriented Sides by High-Speed Atomic Force Microscopy2019

    • Author(s)
      Haruyama, T. Sugano, Y. Kodera, N. Uchihashi, T. Ando, T. Tanaka, Y. Konno, H. Tsukazaki, T.
    • Journal Title

      Structure

      Volume: 27 Pages: 152-160

    • DOI

      10.1016/j.str.2018.09.005

    • NAID

      120006633091

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Free Energy Landscape and Dynamics of Supercoiled DNA by High-Speed Atomic Force Microscopy2018

    • Author(s)
      Brouns, T. De Keersmaecker, H. Konrad, S. F. Kodera, N. Ando, T. Lipfert, J. De Feyter, S. Vanderlinden, W.
    • Journal Title

      ACS Nano

      Volume: 12 Pages: 11907-11916

    • DOI

      10.1021/acsnano.8b06994

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The 2018 correlative microscopy techniques roadmap2018

    • Author(s)
      T. Ando et al. (36 authors in total)
    • Journal Title

      J. Phys. D: Appl. Phys.

      Volume: 51

    • DOI

      10.1088/1361-6463/aad055

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time2018

    • Author(s)
      T. Mori, S. Sugiyama, M. Byme, C. H. Johnson, T. Uchihashi, T. Ando
    • Journal Title

      Nature Communication

      Volume: 9

    • DOI

      10.1038/s41467-018-05438-4

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamic structural states of ClpB involved in its disaggregation function2018

    • Author(s)
      T. Uchihashi, Y. Watanabe, Y. Nakazaki, T. Yamasaki, H. Watanabe, T. Maruno, K. Ishii, S. Uchiyama, C. Song, K. Murata, R. Iino, and T. Ando
    • Journal Title

      Nature Communication

      Volume: 9 Pages: 2147-2147

    • DOI

      10.1038/s41467-018-04587-w

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Real time monitoring of lipid exchange via fusion of peptide based lipid-nanodisc2018

    • Author(s)
      T. Ravula, D. Ishikuro, N. Kodera, T. Ando, G. Anantharamaiah, A. Ramamoorthy
    • Journal Title

      Chem. Mater.

      Volume: 印刷中 Pages: 3204-3207

    • DOI

      10.1021/acs.chemmater.8b00946

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Quantum-dot antibody conjugation visualized at the single-molecule scale with high-speed aomic force microscopy2018

    • Author(s)
      T. Umakoshi, H. Udaka, T. Uchihashi, T. Ando, M. Suzuki
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 167 Pages: 267-274

    • DOI

      10.1016/j.colsurfb.2018.04.015

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Substrate protein dependence of GroEL-GroES interaction cycle revealed by high-speed AFM imaging2018

    • Author(s)
      D. Noshiro and T. Ando
    • Journal Title

      Philosophical Transactions of the Royal Society B: Biological Sciences

      Volume: 印刷中 Pages: 20170180-20170180

    • DOI

      10.1098/rstb.2017.0180

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Insight into structural remodeling of the FlhA ring responsible for bacterial flagellar type III protein export2018

    • Author(s)
      Terahara, N., Inoue, Y., Kodera, N., Morimoto, Y. V., Uchihashi, T., Imada, K., Ando, T., Namba, K. & Minamino, T.
    • Journal Title

      Sciences Advances

      Volume: 4

    • DOI

      10.1126/sciadv.aao7054

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Negatively Charged Lipids Are Essential for Functional and Structural Switch of Human 2-Cys Peroxiredoxin II2018

    • Author(s)
      Haruyama Takamitsu、Uchihashi Takayuki、Yamada Yutaro、Kodera Noriyuki、Ando Toshio、Konno Hiroki
    • Journal Title

      J. Mol. Biol.

      Volume: 430 Pages: 602-610

    • DOI

      10.1016/j.jmb.2017.12.020

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamic clustering of dynamin-amphiphysin helices regulates membrane constriction and fission coupled with GTP hydrolysis2018

    • Author(s)
      Tetsuya Takeda, Toshiya Kozai, Huiran Yang, Daiki Ishikuro, Kaho Seyama, Yusuke Kumagai, Tadashi Abe, Hiroshi Yamada, Takayuki Uchihashi, Toshio Ando, Kohji Takei
    • Journal Title

      elife

      Volume: 7 Pages: 1-19

    • DOI

      10.7554/elife.30246

    • NAID

      120006510224

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-speed atomic force microscopy and its future prospects2018

    • Author(s)
      T. Ando
    • Journal Title

      Biophys. Rev.

      Volume: 10 Pages: 285-292

    • DOI

      10.1007/s12551-017-0356-5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-speed atomic force microscopy (AFM)"2018

    • Author(s)
      T. Ando
    • Journal Title

      Encyclopedia of Biophysics

      Volume: 印刷中 Pages: 1-7

    • DOI

      10.1007/978-3-642-35943-9_478-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy2017

    • Author(s)
      M. Shibata, H. Nishimasu, N. Kodera, S. Hirano, T. Ando, T. Uchihashi & O. Nureki
    • Journal Title

      Nat. Commun.

      Volume: 8 Pages: 1430-1430

    • NAID

      120006374160

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Na+-induced structural transition of MotPS for stator assembly of Bacillus flagellar motor.2017

    • Author(s)
      Terahara, N., Kodera, N., Uchihashi, T., Ando, T., Namba, K. & Minamino, T.
    • Journal Title

      Science Advances

      Volume: 3 Pages: 4119-4119

    • DOI

      10.1126/sciadv.aao4119

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] High-speed XYZ nanopositioner for scanning ion conductance microscopy2017

    • Author(s)
      S. Watanabe and T. Ando
    • Journal Title

      Appl. Phys. Lett.

      Volume: 111 Pages: 113106-113106

    • DOI

      10.1063/1.4993296

    • NAID

      120006370925

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Directly watching biomolecules in action by high-speed atomic force microscopy2017

    • Author(s)
      T. Ando
    • Journal Title

      Biophys. Rev.

      Volume: 9 Pages: 421-429

    • DOI

      10.1007/s12551-017-0281-7

    • NAID

      120006374161

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-Speed Atomic Force Microscopy Reveals Loss of Nuclear Pore Resilience as a Dying Code in Colorectal Cancer Cells.2017

    • Author(s)
      Mohamed MS, Kobayashi A, Taoka A, Watanabe-Nakayama T, Kikuchi Y, Hazawa M, Minamoto T, Fukumori Y, Kodera N, Uchihashi T, Ando T, Wong RW
    • Journal Title

      ACS Nano

      Volume: 11(6) Pages: 5567-5578

    • DOI

      10.1021/acsnano.7b00906

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Imaging modes of atomic force microscopy for application in molecular and cell biology2017

    • Author(s)
      Yves F. Dufrene, Toshio Ando, Ricardo Garcia, David Alsteens, David Martinez-Martin, Andreas Engel, Christoph Gerber and Daniel J. Muller
    • Journal Title

      Nat. Nanotechnol.

      Volume: 12 Pages: 295-307

    • DOI

      10.1038/nnano.2017.45

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] High-speed atomic force microscopy for observing protein molecules in dynamic action2017

    • Author(s)
      T. Ando
    • Journal Title

      Proceedings of SPIE

      Volume: 10328

    • DOI

      10.1117/12.2268795

    • NAID

      120006306248

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chaperonin GroEL-GroES functions as both alternating and non-alternating engines2016

    • Author(s)
      D. Yamamoto and T. Ando
    • Journal Title

      J. Mol. Biol.

      Volume: 428 Pages: 3090-3101

    • DOI

      10.1016/j.jmb.2016.06.017

    • NAID

      120005851370

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] The Intrinsically Disordered Protein Atg13 Mediates Supramolecular Assembly of Autophagy Initiation Complexes.2016

    • Author(s)
      Yamamoto H, Fujioka Y, Suzuki SW, Noshiro D, Suzuki H, Kondo-Kakuta C, Kimura Y, Hirano H, Ando T, Noda NN, Ohsumi Y
    • Journal Title

      Dev. Cell

      Volume: 38 Pages: 86-89

    • DOI

      10.1016/j.devcel.2016.06.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] High-speed atomic force microscopy reveals strongly polarized movement of clostridial collagenase along collagen fibrils2016

    • Author(s)
      T. Watanabe-Nakayama, M. Itami, N. Kodera, T. Ando, H. Konno,
    • Journal Title

      Scientific Reports

      Volume: 4 Pages: 1-11

    • DOI

      10.1038/srep28975

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Functional extension of high-speed AFM for wider biological applications.2016

    • Author(s)
      Uchihashi T, Watanabe H, Fukuda S, Shibata M, Ando T.
    • Journal Title

      Ultramicroscopy

      Volume: 160 Pages: 182-196

    • DOI

      10.1016/j.ultramic.2015.10.017

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Method of mechanical holding of cantilever chip for tip-scan high-speed atomic force microscope.2015

    • Author(s)
      Fukuda S, Uchihashi T, Ando T.
    • Journal Title

      Rev. Sci. Instrum.

      Volume: 86 Pages: 063703-063703

    • DOI

      10.1063/1.4922381

    • NAID

      120005647653

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] High-speed AFM: directly visualizing protein molecules during their functional activity2019

    • Author(s)
      T. Ando
    • Organizer
      15th JSPS German and Japanese Colloquium: Nano-LifeScience ((Harnack Hous of the Max Planck Institute, Berlin)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Protein machinery enabling life2019

    • Author(s)
      T. Ando
    • Organizer
      Max von Laue Colloquium of the Physical Society of Berlin (Physikalisch-Technische Bundesanstalt Hörsaal im Hermann-von-Helmholtz-Bau, Berlin)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed atomic force microscopy for observing biological molecules in dynamic action2019

    • Author(s)
      T. Ando
    • Organizer
      Seminar at the Fritz Harber Institute of the Max Planck Society (Berlin)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamic processes in pore-forming Streptolysin O on lipid membrane2019

    • Author(s)
      T. Ando
    • Organizer
      XXI Linz Winter Workshop (Bio-AFM Workshop (Johaness Kepler University Linz, Linz Austria)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed AFM and SICM: Watching biological samples in dynamic action2018

    • Author(s)
      T. Ando
    • Organizer
      Bio-AFM Workshop on "AFM and Related Techniques for Biological Research" (Empa, St. Gallen, Switzerland)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Keynote speech "High-speed AFM: Visializing protein molecules during their functional activity"2018

    • Author(s)
      T. Ando
    • Organizer
      19th International Microscopy Congress (Sydney, Australia)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 高速AFMによる分子・細胞の高解像ダイナミクスイメージングとその展望2018

    • Author(s)
      安藤敏夫
    • Organizer
      第124回日本解剖学会総会・全国学術集会(新潟朱鷺メッセ)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 特別講演「動的な分子レベルの生命現象を直視する高速AFM」2018

    • Author(s)
      安藤敏夫
    • Organizer
      平成30年度金沢大学がん進展制御研究所共同利用・共同研究拠点共同研究成果報告会(金沢大学)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 基調講演「生体分子機械の機能動作を活写する高速AFM」2018

    • Author(s)
      安藤敏夫
    • Organizer
      日本電子顕微鏡学会第61回シンポジウム(富山国際会議場)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 高速AFMの機能拡張に向けた技術開発2018

    • Author(s)
      安藤敏夫
    • Organizer
      JASRIワークショップ「高分解能イメージング技術」(Spring-8)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 特別講演「タンパク質・細胞の動態を活写する高速AFM」2018

    • Author(s)
      安藤敏夫
    • Organizer
      JST/さきがけ「微粒子」第2回領域会議(TKP金沢カンファレンスセンター)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 特別講演「動作中の蛋白質分子の高速AFMによる直接観察」2018

    • Author(s)
      安藤敏夫
    • Organizer
      平成30年度関東研究医養成コンソーシアム夏のリトリート(金沢商工会議所)
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 蛋白質分子の機能動態を直接撮影する高速AFM2018

    • Author(s)
      安藤敏夫
    • Organizer
      第18回蛋白質科学会年会 シンポジウム「蛋白質立体構造解析のフロンティア」(新潟朱鷺メッセ)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamic GroEL-GroES Interaction and Its Dependence on Substrate Protein2018

    • Author(s)
      T. Ando
    • Organizer
      XX Linz Winter Workshop
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Keynote speech: High-speed AFM for filming protein molecules in action2017

    • Author(s)
      T. Ando
    • Organizer
      8th INternational Conference on Advanced Materials and Nanotechnology
    • Place of Presentation
      Queenstown, New Zealand
    • Year and Date
      2017-02-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] GroEL-GroES interaction cycle revealed by high-speed AFM2017

    • Author(s)
      T. Ando and D. Yamamoto
    • Organizer
      XVIII Linz Winter Workshop
    • Place of Presentation
      Johannes Kepler University Linz, Linz Austria
    • Year and Date
      2017-02-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed atomic force microscopy: Visualizing protein molecules in action2017

    • Author(s)
      T. Ando
    • Organizer
      Physics & Life Science Colloquium (Lawrence Livermore National Laboratory)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Structural analysis of IDPs by their visualization with high-speed AFM2017

    • Author(s)
      T. Ando
    • Organizer
      CECAM Workshop on "Disordered protein segments: revisiting the structure-function paradigm"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Special Lecture "Nano-visualization of protein molecules in action by high-speed AFM2017

    • Author(s)
      T. Ando
    • Organizer
      15th International Conference on Na, K-ATPase and Related Transport ATPases
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Directly watching biomolecules in action by high-speed atomic force microscopy2017

    • Author(s)
      T. Ando
    • Organizer
      19th IUPAB Congress & 11th EBSA Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Direct visualization of biological nanomachines in action by high-speed AFM2017

    • Author(s)
      T. Ando
    • Organizer
      9th International Conference on Engineering of Chemical Complexity
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Dynamic GroEL-GroES interaction revealed by high-speed atomic force microscopy2017

    • Author(s)
      T. Ando
    • Organizer
      Royal Society Discussion Meeting on Allostery and Molecular Machines
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed atomic force microscopy and its future prospects2017

    • Author(s)
      T. Ando
    • Organizer
      19th International Scanning Probe Microscopy Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 特別講演 タンパク質分子の機能動態を直接撮影する高速AFM2017

    • Author(s)
      安藤敏夫
    • Organizer
      日本薬学会「生体膜と薬物の相互作用」シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Dynamic actin-myosin interaction visualized by high-speed AFM2017

    • Author(s)
      安藤敏夫
    • Organizer
      第81回日本循環器学会学術集会シンポジウム「心血管分子イメージングの未来:基礎ー臨床の連携」
    • Place of Presentation
      ホテル日航金沢
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Observation of S. solfataricus archaeal minichromosome maintenance (ssoMCM) protein complex by high-speed AFM2016

    • Author(s)
      D. Noshiro, N. Kodera, T. Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] High-speed AFM Observation of Membrane Pore Formation by Streptolysin2016

    • Author(s)
      H. Ariyama, N. Kodera, T. Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Dynamics observation of the 20S proteasome-related proteins using High-Speed AFM2016

    • Author(s)
      T. Kozai, T. Satoh, A. Sikdar, H. Yagi, M. Yagi-Utsumi, T. Uchihashi, T. Ando, K. Kato
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] High-speed atomic force microscopy shows conformational changes of nucleic binding domains of ABC protein2016

    • Author(s)
      S. Takigaura, M. Shibata, K. Yamahara, Y. Kimura, K. Ueda, T. Uchihashi1, T. Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] The extent of unidirectional cooperativity in rotorless enterococcus hirae V1-ATPase revealed by high-speed AFM2016

    • Author(s)
      M. Imamura, K. Nakamoto, S. Maruyama, F. Kawai, R. Iino, T. Uchihashi, T. Murata, T. Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Combined system of high speed atomic force microscopy(HS-AFM) and optical tweezers2016

    • Author(s)
      S. Yamanaka1, A. Goto, H. Watanabe, T. Uchihashi, T. Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Development of high-speed near-field optical microscopy2016

    • Author(s)
      T. Umakoshi, S. Fukuda, T. Uchihashi, T. Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Observation of conformational dynamics of FliI by HS-AFM2016

    • Author(s)
      K. Adachi, J. Kishikawa, H. Terashima, T. Uchihashi, K. Imada, K. Yokoyama, T. Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Visualization of protein molecules in action by high-speed atomic force microscopy2016

    • Author(s)
      M. Shibata, N. Kodera, T. Uchihashi, T. Ando
    • Organizer
      日本生物物理学会第54回年会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] High-speed atomic force microscopy and its prospects for biological research2016

    • Author(s)
      T. Ando
    • Organizer
      Symposium for exploring prospective research -Pioneering New Fields: Forefront of RIKEN's Science and Beyond-
    • Place of Presentation
      RIKEN Wako campus
    • Year and Date
      2016-11-21
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] High-speed atomic force microscopy for observing protein molecules in dynamic action2016

    • Author(s)
      T. Ando
    • Organizer
      International Congress on High-speed Imaging and Photonics
    • Place of Presentation
      Hankyu Hotel Expo Park, Osaka
    • Year and Date
      2016-11-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed AFM visualization of structure, function and dysfunction of intrinsically disordered proteins2016

    • Author(s)
      T. Ando
    • Organizer
      4th Kanazawa Bio-AFM Workshop
    • Place of Presentation
      KKR Hotel Kanazawa
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Plenary talk: High-speed atomic force microscopy for biomolecular and cellular studies2016

    • Author(s)
      T. Ando
    • Organizer
      SPM on SPM 2016
    • Place of Presentation
      Jilin University, Chungchun, China
    • Year and Date
      2016-08-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Watching the structure, function and dysfunction of IDPs by high-speed atomic force microscopy2016

    • Author(s)
      T. Ando
    • Organizer
      Intrinsically Disordered Proteins Gordon Research Conference
    • Place of Presentation
      Les Diablerets, Switzerland
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Functional extension of high-speed atomic force microscopy2016

    • Author(s)
      T. Ando, N. Kodera, T. Uchihashi, S. Watanabe, Takamitsu Haruyama and Hiroki Konno
    • Organizer
      ISPM 2016
    • Place of Presentation
      Grindelwald, Switzerland
    • Year and Date
      2016-06-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 天然変性蛋白質の構造・機能の高速AFM解析2016

    • Author(s)
      安藤敏夫
    • Organizer
      日本蛋白質科学会 第16回年会 ワークショップ「天然変性タンパク質研究の深まりと広がり」
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2016-06-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] High-speed force microscopy to watch nanoscale dynamics2016

    • Author(s)
      T. Ando, N. Kodera & T. Uchihashi
    • Organizer
      6th Multifrequency AFM Conference
    • Place of Presentation
      Madrid, Spain
    • Year and Date
      2016-03-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質の動態を可視化する高速AFM2016

    • Author(s)
      安藤敏夫
    • Organizer
      第2回生物構造学研究会
    • Place of Presentation
      飯田橋、東京
    • Year and Date
      2016-03-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Structural and functional analyses of IDPs by high-speed AFM imaging2016

    • Author(s)
      T. Ando & N. Kodera
    • Organizer
      Sub-group meeting of intrinsically disordered proteins at the Biophysical Society 60th annual meeting
    • Place of Presentation
      Los Angeles, USA
    • Year and Date
      2016-02-27
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Watching dynamic phenomena occurring in liquids at the nanometer scale by using high-speed atomic force microscopy2016

    • Author(s)
      T. Ando
    • Organizer
      Seminar at Technion
    • Place of Presentation
      Technion, Haifa, Israel
    • Year and Date
      2016-02-24
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Keynote speech "Nano-visualization of biomolecules in dynamic action by high-speed atomic force microscopy"2016

    • Author(s)
      T. Ando
    • Organizer
      Nano Israel 2016
    • Place of Presentation
      Tel Aviv University, Tel Aviv Israel
    • Year and Date
      2016-02-22
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed AFM imaging of protein molecules in dynamic action2016

    • Author(s)
      T. Ando, T. Uchihashi, N. Kodera & D. Yamamoto
    • Organizer
      XVII Linz Winter Workshop
    • Place of Presentation
      Johannes Kepler University Linz, Linz Austria
    • Year and Date
      2016-01-29
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Direct visualization of protein molecules in dynamic action by high-speed atomic force microscopy2015

    • Author(s)
      T. Ando
    • Organizer
      The 25th Hot Spring Harbor Internationl Symposium
    • Place of Presentation
      Kyushu University, Fukuoka Japan
    • Year and Date
      2015-11-13
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Direct visualization of protein molecules in dynamic action by high-speed atomic force microscopy2015

    • Author(s)
      T. Ando
    • Organizer
      JST CREST-PREST Jouint International Symposium
    • Place of Presentation
      Univ. of Tokyo, Tokyo, Japan
    • Year and Date
      2015-11-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speedd AFM observation of membrane pore formation by streptolysin O2015

    • Author(s)
      H. Ariyama, N. Kodera, T. Ando
    • Organizer
      日本生物物理学会第53回年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] Improvement of tip-scan HS-AFM for live-cell imaging2015

    • Author(s)
      S. Yamanaka, H. Watanabe, T. Uchihashi, T. Ando
    • Organizer
      日本生物物理学会第53回年会
    • Place of Presentation
      金沢大学
    • Year and Date
      2015-09-13
    • Related Report
      2015 Annual Research Report
  • [Presentation] High-speed atomic force microscopy for observing biological molecules in dynamic action2015

    • Author(s)
      T. Ando
    • Organizer
      2015 NIMS Conference
    • Place of Presentation
      Tsukuba, Japan
    • Year and Date
      2015-07-14
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] ダイナミックに動作中のタンパク質分子を直接可視化する高速AFM2015

    • Author(s)
      安藤敏夫
    • Organizer
      第15回日本蛋白質科学会年会 ワークショップ
    • Place of Presentation
      あわぎんホール、徳島
    • Year and Date
      2015-06-24
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] High-speed AFM for nano-visualization of dynamic processes2015

    • Author(s)
      T. Ando
    • Organizer
      The Nano TR11
    • Place of Presentation
      Middle East Technical University, Ankara, Turkey
    • Year and Date
      2015-06-22
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] タンパク質分子の構造・機能動態を活写する高速AFM2015

    • Author(s)
      安藤敏夫
    • Organizer
      大阪大学蛋白質研究所セミナー
    • Place of Presentation
      蛋白研、大阪大学
    • Year and Date
      2015-06-18
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 特別講演「生体分子・細胞を活写する高速AFM」2015

    • Author(s)
      安藤敏夫
    • Organizer
      新学術領域研究「運動超分子が織りなす調和と多様性」第3回領域全体会議プログラム
    • Place of Presentation
      金沢商工会議所会館ホール
    • Year and Date
      2015-06-10
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] 生体分子の構造・動作を活写する高速AFM2015

    • Author(s)
      安藤敏夫
    • Organizer
      名古屋大学大学院マイクロ・ナノ工学研究科特別講義
    • Place of Presentation
      名古屋大学
    • Year and Date
      2015-05-14
    • Related Report
      2015 Annual Research Report
    • Invited
  • [Presentation] Direct visualization of dynamic biomolecular and cellular processes by high-speed atomic force microscopy2014

    • Author(s)
      T. Ando
    • Organizer
      Distinguished Lecture in Biological Engineering at the EPFL-SV-IBI
    • Place of Presentation
      EPFL, Lausanne, Switzerland
    • Year and Date
      2014-10-20
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] 高速AFM/1分子蛍光顕微鏡複合機によるタンパク質の構造動態と化学反応の同時イメージング2014

    • Author(s)
      福田真悟、内橋貴之、飯野亮太、安藤敏夫
    • Organizer
      日本生物物理学会第52回年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Book] "High-speed Atomic Force Microscopy", Chapter 22, pp.481-518 in Noncontact Atomic Force Microscopy Vol.32015

    • Author(s)
      T. Uchihashi, N. Kodera, T. Ando
    • Total Pages
      527
    • Publisher
      Springer
    • Related Report
      2015 Annual Research Report
  • [Remarks] 金沢大学ナノ生命科学研究所 研究成果の紹介

    • URL

      https://nanolsi.kanazawa-u.ac.jp/achievements/

    • Related Report
      2018 Annual Research Report
  • [Remarks] Key Finding, Nano Life Science Institute

    • URL

      https://nanolsi.kanazawa-u.ac.jp/en/achievements/

    • Related Report
      2018 Annual Research Report
  • [Remarks] プレスリリース 金沢大学ナノ生命科学研究所

    • URL

      https://nanolsi.kanazawa-u.ac.jp/category/announcements/press/

    • Related Report
      2018 Annual Research Report
  • [Remarks] Press Release Nano Life Science Institute

    • URL

      https://nanolsi.kanazawa-u.ac.jp/en/category/announcements/press/

    • Related Report
      2018 Annual Research Report
  • [Remarks] Welcome to Kanazawa Biophysics Lab

    • URL

      http://biophys.w3.kanazawa-u.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 金沢大学 生物物理学研究室へようこそ

    • URL

      http://biophys.w3.kanazawa-u.ac.jp/index_J.htm

    • Related Report
      2017 Annual Research Report 2016 Annual Research Report
  • [Remarks] NanoLSI WPI Kanazawa University

    • URL

      https://nanolsi.kanazawa-u.ac.jp/en/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 金沢大学ナノ生命科学研究所

    • URL

      https://nanolsi.kanazawa-u.ac.jp/

    • Related Report
      2017 Annual Research Report
  • [Remarks] Welcome to Kanazawa Biophysics Lab

    • URL

      http://biophys.w3.kanazawa-u.ac.jp/index.htm

    • Related Report
      2016 Annual Research Report
  • [Remarks] Bio-AFM Frontier Research Center

    • URL

      http://www.se.kanazawa-u.ac.jp/bioafm_center/index.htm

    • Related Report
      2016 Annual Research Report 2014 Annual Research Report
  • [Remarks] バイオAFM先端研究センター

    • URL

      http://www.se.kanazawa-u.ac.jp/bioafm_center/j/index.htm

    • Related Report
      2016 Annual Research Report 2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] Bio-AFM Frontier Research center

    • URL

      http://www.se.kanazawa-u.ac.jp/bioafm_center/index.htm

    • Related Report
      2015 Annual Research Report
  • [Remarks] Welcome to Kanazawa Biophysics Lab

    • URL

      http://www.s.kanazawa-u.ac.jp/phys/biophys/

    • Related Report
      2015 Annual Research Report
  • [Remarks] 金沢大学 生物物理学研究室へようこそ

    • URL

      http://www.s.kanazawa-u.ac.jp/phys/biophys/index_J.htm

    • Related Report
      2015 Annual Research Report 2014 Annual Research Report
  • [Remarks] Welcome to Kanazawa Biophysics Lab

    • URL

      http://www.s.kanazawa-u.ac.jp/phys/biophys/index.htm

    • Related Report
      2014 Annual Research Report
  • [Funded Workshop] Bio-AFM Summer School (Kanazawa Univ.)2018

    • Related Report
      2018 Annual Research Report
  • [Funded Workshop] 4th Kanazawa Bio-AFM Workshop2016

    • Place of Presentation
      KKR Hotel Kanazawa
    • Year and Date
      2016-10-03
    • Related Report
      2016 Annual Research Report

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Published: 2014-11-20   Modified: 2022-01-28  

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