Isolation and Comparative Physiological Studies of Aerobic PhotoーSynthetic Bacteria from Tropical Sea Area of Australia
Grant-in-Aid for international Scientific Research
|Allocation Type||Single-year Grants |
|Research Institution||Kyushu University |
TAKAMIYA Ken-ichiro Dept. Biol. , Facl. Sci. , Kyushu Univ. , Associate Professor, 理学部, 助教授 (80037259)
SUTTON David C. James Cook Univ. , Sir. George Fisher Center Trop. Marine Inst. Research Associa, 熱帯海洋研究所, 助手
WILKINSON Cl オーストラリア海洋研究所, 主席研究員
KOHATA Kunio National Inst. Environ. Studies, Senior Researcher, 主任研究員 (30132848)
SHIBA Tsuneo Otsuchi Marine Res. Center, Tokyo Univ. , Research Associate, 大槌臨海研究センター, 助手 (20126080)
SHIOI Yuzo Div. Biol. , Facl. Med. , Miyazaki Med. Col. , Associate Professor, 医学部, 助教授 (70094092)
WILKINSON Clive R. Australian Inst. Marine Sci. , Principal Investigator
|Project Period (FY)
1989 – 1990
Completed (Fiscal Year 1990)
|Budget Amount *help
¥15,000,000 (Direct Cost: ¥15,000,000)
Fiscal Year 1990: ¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1989: ¥7,500,000 (Direct Cost: ¥7,500,000)
|Keywords||Aerobic photosynthetic bacteria / Australia / Bacteriochlorophyll / hetero-trophic bacteria / Cytochromes / Reaction center / Denitrification / Stromatolite|
Aerobic heterotrophic bacteria containing bacteriochlorophyll a were isolated from specimens from a wide variety of marine environments on the coast of Australia (on the west cost, Shark Bay, Lake Clifton, Lake Heyward and Perth ; on the east coast, near Townsville and Brisbane ; on the north coast, near Darwin ; on the south coast, near Adelaide and Tasmania Island).
The bacteria were found in a high proportion (10 to 30%) of the total hetero-trophic bacterial strains isolated from marine algae, seagrasses, stromatolites, the epiphites on stromatolites, and from seawater and sands ; in some cases they constituted up to 49% of the total. This is much higher than the previous report of 6% from Japan. A high percentage, 13%, was also found in the seawater of Hamelin Pool, at Shark Bay, where the salinity was 66%. The proportion of these bacteria was generally low in seawater and sands, with a few exceptions. There were no bacteriochlorophyll-containing bacteria on sponges or corals. Finally about 300 strains were found to contain bacteriochlorophyll a.
The isolated strains were orange or pink, and most had absorption maxima around 800 and 850 to 870 nm, the absorption of bacteriochlorophyll in vivo.
The maximum bacteriochlorophyll content was 1 nmol/mg dry wt. of bacterial cells. Most of the bacteria did not grow photoheterotrophically under anaeroーbic conditions in a broth medium containing succinate.
Cells and cell extracts grown under aerobic conditions had photochemical activities such as reversible photooxidations of the reaction center and cytochrome(s). Some strains showed denitrifying activity. The optimal salinity for bacterial growth varied between strains.
Report (1 results)
Research Products (22 results)