Grant-in-Aid International Scientific Research
|Allocation Type||Single-year Grants|
|Research Institution||The Kagawa Medical School|
HATASE Osamu The Kagawa Medical School・Professor, 医学部, 教授 (50033220)
SHARMA Rajen アルバータ州立カルガリ大学, 医学部, 助教授
WAISMAN David M. The University of Calgary・Associate Professor, 医学部, 助教授
WANG Jerry H. The University of Calgary・Professor, 医学部, 教授
TOKUDA Masaaki The Kagawa Medical School・Research Associate, 医学部, 助手 (10163974)
ITANO Toshifumi The Kagawa Medical School・Research Associate, 医学部, 助手 (60145042)
MATSUI Hideki The Kagawa Medical School・Associate Professor, 医学部, 助教授 (30157234)
SHARAM Rajendra K. The University of Calgary・Associate Professor
|Project Fiscal Year
1989 – 1991
Completed(Fiscal Year 1991)
|Budget Amount *help
¥7,400,000 (Direct Cost : ¥7,400,000)
Fiscal Year 1991 : ¥1,700,000 (Direct Cost : ¥1,700,000)
Fiscal Year 1990 : ¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 1989 : ¥2,200,000 (Direct Cost : ¥2,200,000)
|Keywords||Calcium Binding Protein / Calcineurin / Calmodulin / Lipocortin / カルシニュウリン|
Three Ca^<2+>-binding proteins in brain, calcineurin, phosphodiesterase and lipocortins, were studied and following results were obtained.
l. calcineurin (CaN)
・Monoclonal antibodies against alpha and beta were produced.
・Immunohistochemical study elucidated the distinct distribution of both subunits in brain.
・A novel beta subunit (beta_1) from rat testis was cloned and amino acid sequence was determined. Six additive hydrophilic amino acids were located in the carboxyl terminal of the molecule as compared to the bovine type beta subunit.
・Two isoforms of alpha subunit of bovine brain CaN were demonstrated by Western blot analysis using the monoclonal antibody.
・A part of CaN could translocate from cytosoi to membrane in a Ca^<2+> dependent manner.
2. phosphodiesterase (PDE)
・A monclonal antibody was raised.
・Two isozymes, PDE_1, (Mr=63, 000) and PDE_2 (Mr=60, 000), were identified.
・PDE_1 and PDE_2 were phosphorylated by protein kinase C and cyclic-AMP dependent protein kinase, respectively.
・PDE_1 and PDE_2 showed a distinct distribution in rat brain.
3. lipocortins (LP)
・Monoclonal antibodies against LP-I (Mr=35, 000) and LP-II (Mr=36, 000) were produced.
・Both LP-I and LP-II inhibited phospholipase A_2 activity, and phosphorylated forms of both proteins by protein kinase C showed a reduced inhibitory activity on phospholipase A_2.
・LP-I and LP-11 showed a developmental change in amount ; higher quantity in newborn rat brain and lower in adult brain.
・Specific distribution in astrocytes (not in neurons) was confirmed.