山田 英俊 徳島文理大学, 薬学部, 助手 (90200732)
西沢 麦夫 徳島文理大学, 薬学部, 教授 (40137188)
松本 武 ダイゼル化学工業株式会社, 総合研究所, 主任研究員
徳山 孝 大阪市立大学, 理学部, 教授 (70046944)
カイルー ソフニ インドネシア国立原子力研究センター, 研究員
カイルー インドネシア科学院, 生物学研究センター, 主任研究員
ソフニ カイルー インドネシア国立原子力研究センター, 研究員
CHAIRUL National Centre for Research in Biology, Indonesian Institute of Science (LIPI)
CHAIRUL Sofni M Centre for the Application of Isotopes and Radiation, Indonesian Atomic Energy A
TOKUYAMA Takashi Faculty of Science, Osaka City University
MATSUMOTO Takeshi Research Institute, DAICEL Chemical Industries, Co.
YAMADA Hidetoshi Faculty of Pharmaceutical Sciences, Tokushima Bunri University
NISHIZAWA Mugio Faculty of Pharmaceutical Sciences, Tokushima Bunri University
|Budget Amount *help
¥12,500,000 (Direct Cost : ¥12,500,000)
Fiscal Year 1991 : ¥4,000,000 (Direct Cost : ¥4,000,000)
Fiscal Year 1990 : ¥4,000,000 (Direct Cost : ¥4,000,000)
Fiscal Year 1989 : ¥4,500,000 (Direct Cost : ¥4,500,000)
This project concerns our cooperative program with the Research Centre for Biology, Indonesian Institute of Science (LIPI), since 1982 titled "Chemical Studies on Biologically Active Principles of Tropical Plants and Animals in Indonesia".
In the first year (1989), we have a research activity in Central Sulawesi, looking for poisonous and medicinal plants and animals in wilder area, according to the informations of Dr. B. Soeroto of Sam Ratulangi Univ., Manado. At Watusongu village of a primitive Tah tribe, about 300 km northeast of Poso, Central Sulawesi, we have collected ca. 50 species of poisonous and medicinal plants, which included three practically used strong poisons, one arrow-poison (local name : "Impoh", latex), two fish-poisons ("Luli", seeds and "Tuwa", fresh roots). Together with these, ca. 20 species of Polyporaceous mushrooms and some quantity of skin of an Indonesian toad, Bufo sulawesiensis. Arrow-poison frogs, so called "green poison frog", were also tried to collect,
but this was not successful in this year. A remarkable anti-tumor promoter activity was found from nine of the ca. 50 plant species collected and the results was preliminarily presented at 50th Annual Meeting of Japan Cancer Association (1991, Tokyo). The most expected three plants described above, Impoh, Luli and Tuwa, were identified at Bogor Herbarium as Antiaris toxocaria (Urticaceae), Croton tiglium (Euphorbiaceae), and Derris elliptica (Leguminosae), respectively, and their active principles have already been investigated, regrettably, by German chemists before 1970s. Mushroom components have been analyzed chemically and biologically, and applanoxidic acids A -D, isolated from Ganoderma applanatum (Polyporaceae), were reported as anti-tumor promoter components on "Phytochemistry (1991)" and at the Symposium on Medicinal Resources of Tropical Rain Forest (1991, Kuala Lumpur, Malaysia).
In the second year activity (1990), we have visited Watusongu village again, and looked for the "green poison frog" for a week by tight cooperation with local people. However, this time was also unsuccessful, unfortunately. Thorough search by longer stay should be essential for such movable animals in the future activity. At the same time, some additional quantity of the promising plants and mushrooms in the last year activity were also collected.
The last year (1991) of this project was mainly alloted for processing the remaining materials collected, presentation of the results, and discussion on the future program with LIPI. Other than these, a field activity was also carried out at Lake Toba, for collection of Bufo toads in North Sumatra for comparison of the skin components with those in other areas. The results on the toad components in Jawa and Sulawesi were presented at 61st Annual Meeting of Japan Chemical Society (1991, Yokohama) and 33rd Symposium on the Chemistry of Natural Products (1991, Osaka). The joint program with LIPI was decided to be renewed for additional two years, from April, 1992, to March, 1994, by the agreement with the Director of the Research Centre for Biology, LIPI, in Bogor. Less