和田 昭允 東京大学, 理学部, 教授 (10011462)
WATANABE Sadamoto University of Tokyo, Faculty of Agriculture, Professor, 農学部, 教授 (30182918)
MIYANO Kenjiro University of Tokyo, Faculty of Engineering, Associate Professor, 工学部, 助教授 (90167677)
MATSUOKA Hideaki Tokyo University of Agriculture and Technology, Faculty of Technology, Professor, 工学部, 教授 (10143653)
EIHO Shigeru Kyoto University, Faculty of Engineering, Professor, 工学部, 教授 (40026117)
KUBO Izumi Soka University, Institute of Life Science, Associate Professor, 生命科学研究所, 助教授 (40214986)
Chemical communication between plants is an attractive subject from the viewpoint of remote chemical interface. This study describes the possible role of essential oil components extracted from young buds of white birch (Betula platyphylla) in the control of seed germination of the same plant. When the concentration of essential oil components such as geraniol was high in gas phase, the germination was retarded. In contrast, it was promoted by low concentration of those compounds. It was also found that such chemicals were secreted from the seeds themselves, as might control the seed germination. From the water soluble fractions, four components were separated as determined by HPLC. Two of them showed inhibitory effect and retarded the germination 9-12 hours. At the same time the germination rate wa suppressed. The assigment of these components, however, has not yet been performed. On line monitoring of effective components secreted from seeds, especially volatile components, is very difficult by conventional method such as gas chromatography. The monitoring system of these components were established using surface acoustic wave (SAW) device. At first, the surface of SAW device was covered with membrane lipids, which was applied to them. Alcohols and Aldehydes were determined above 10ppm. By covering SAW device with ordered lipids membranes such as Langmuir-Blodgett membrane, the detection limit was improved to ppm. The response of the SAW devices are different each other depending covering lipid. This results gave some good aspects to the assignment of volatile compounds.
特に揮発成分に関しては,従来からのガスクロマトグラフィ-のような方法では,逐次分析が困難であるためsurface acoustic wave(SAW)デバイスによる検出系の確立を試みた。第一に,SAWデバイス上を各種脂質で被覆することにより,低分子アルコ-ルやアルデヒドを数10ppm 以上で検出できるという成果を得た。次に,膜脂質をLB膜化して配向させてSAWデバイスを被覆することにより,数ppm以上で検出が可能となった。また,被覆する膜脂質の種類ごとに個々の化学質への応答が異なることから,複数の膜脂質被覆デバイスの応答パタ-ンで,化学物質の同定も可能となる見通しを得た。