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Induction of Heat Shock Proteins (HSPs) in the Process of Heat Tolerance

Research Project

Project/Area Number 01480134
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 環境生理学(含体力医学・栄養生理学)
Research InstitutionInstitute of Tropical Medicine, Nagasaki University

Principal Investigator

KOSAKA Mitsuo  Institute of Tropical Medicine Environmental Physiology, 熱帯医学研究所, 教授 (30079983)

Co-Investigator(Kenkyū-buntansha) OHWATARI Nobu  Institute of Tropical Medicine Environmental Physiology, 熱帯医学研究所, 助手 (80128165)
MATSUMOTO Takaaki.  Institute of Tropical Medicine Environmental Physiology, 熱帯医学研究所, 助手 (60199875)
山下 俊一  長崎大学, 医学部, 助手 (30200679)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1990: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1989: ¥4,300,000 (Direct Cost: ¥4,300,000)
Keywordsheat acclimatization / heat shock proteins (HSPs) / SDS-PAGE / cytosol fraction / western blotting method / HSP 70 / cold shock proteins (CSPs) / Hyperthermic Oncology / HSP70 / Hyperthermic Oncology / 暑熱・寒冷順化機序 / 温熱感受性と耐熱性 / 熱ショック蛋白質(HSPs) / 体温調節反応 / 急性温熱ショック / 慢性温熱ショック / ウサギ・ラット・ナキウサギ / 電気泳動法(10%SDS-PAGE)
Research Abstract

The aim of this study is to identify the inductions of HSPs (heat shock proteins) and CSPs (cold shock proteins) in weak heat-and strong cold-tolerant pika in order to clarify the mechanism of its character of weak heat-tolerance and to examine the physiological correlation between thermotolerance and HSPs, or CSPs (1) HSP 70 in rats and HSP 68 in rabbits were induced by the heat-shock. In pika rabbits, the induction of HSP by the heat-shock was not detected by 10% SDS-PAGE not only in liver tissue but also in those tissues such as kidney, adrenal gland, spleen, brain and skeletal muscle. According to previous research, tissues of liver, kidney, adrenal gland and spleen have relatively large ability of inducing HSP 70 than other tissues in hyperthermia of rat. In further study, Western blotting method with anti-HSP 70 antibody was performed. HSP 70 was detected in liver tissue of all rats under heat shock, but only one showed positive in all pikas. These results suggest that the diffic … More ulty of induction of HSPs somewhat relate no the characteristic of weak heat-tolerance in pika rabbits. (2) Induction of HSP 68 by chronic heat load instead of heat-shock was observed on 10% SDS-PAGE. In the experiment of Western blotting method with anti-HSP 70 antibody, the 68 kD protein responded to anti-HSP 70 antibody but the spot of staining showed smaller M. W. size. This discrepancy may be explained as a gradation of proteins including 68 kD protein. (3) Contrarily in cold-shock experiments, induction of CSP was not identified in rat, rabbit or pika. But one result was found that 32 kD protein in cytosol fraction vanished in one rat after cold-shock. Four kinds of plasma proteins (55, 27, 25, 20 kD) vanished in chipmunk, a hybernator, during and after the period of hybernation has been reported in previous paper. Therefore, the present finding showed the similarity to the previous report of chipmunk and suggests that not only hybernation but also short term of cold-shock may lead to the similar result. Furthermore, physiological significance of HSP and CSP was discussed from the viewpoint of thermal acclimation. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] Kosaka,M.et al.: "Physiological analysis of weak thermoーtolerance in pika rabbits." XXth International Congress of Neurovegetative Research. Abstracts. 59- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kosaka,M.et al.: "Physiological analysis of weak thermoーtolerance in pika rabbits." Jan.J.Physiol.40. 280- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kosaka,M.et al.: "Analysis of thermoregulatory responses due thermal stimulation in the pika rabbit." Int.J.Biometeorol.34(2). 133- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsumoto,T.et al.: "Seasonal variation of thermal sweating." Trop.Med.32(2). 78-80 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 大渡 伸 他: "アルゴンレ-ザによるウサギの脊髄局所加温と熱放散反応" 日本生理学雑誌. 53(3). 112- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 李 嘉明 他: "ナキウサギ(PiKa)による温熱耐性の温熱生理学的解析" 日本ハイパ-サ-ミア学会誌. 6(3). 343- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohwatari,N. et al.: "Influence of local spinal heating on body temperature in the rabbit.In.Hyperthermic Onocology in Japan '89" Ed.by Masao Saito,Tokyo Univ.press, 320-321 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsumoto,T.et al.: "Changes in circulating hormone levels induced by whole body hyperthermia in man.In.Hyperthermic Oncology in Japan '89" Ed.by Masao Saito, okyo Univ.press, 312-313 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kosaka, M. et al.: "Physiological analysis of weak thermotolerance in pika rabbits." XXth International Congress of Neurovegetative Research, Abstracts. 59. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kosaka, M. et al.: "Physiological analysis of weak thermotolerance in pika rabbits." Jpn. J. Physiol.40. 280 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kosaka, M. et al.: "Analysis of thermoregulatory responses due thermal stimulation in the pika rabbit." Int. J. Biometeorol.34(2). 133 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] MATSUMOTO, T. et al.: "Seasonal variation of thermal sweating." Trop. Med.32(2). 73-80 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohwatari, N. et al.: "Influence of local spinal heating on body temperature in the rabbit. Ed. by Masao Saito, Tokyo Univ. press." Hyperthermic Oncology in Japan '89. 320-321 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsumoto, T. et al.: "Changes in circulating hormone levels induced by whole body hyperthermia in man. Ed. by Masao Saito, Tokyo Univ. press" Hyperthermic Oncology in Japan '89. 312-313 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kosaka,M.et al.: "Physiological analysis of weak thermoーtolerance in pika rabbits." XXth International Congress of Neurovegetative Research. Abstracts. 59 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kosaka,M.et al.: "Physiological analysis of weak thermoーtolerance in pika rabbits." Jpn.J.Physiol.40. 280 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kosaka,M.et al.: "Analysis of thermoregulatory responses due thermal stimulation in the pika rabbit." Int.J.Biometeorol.34(2). 133 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsumoto,T.et al.: "Seasonal variation of thermal sweating." Trop.Med.32(2). 73-80 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 大渡 伸他: "アルゴンレ-ザによるウサギの脊髓局所加温と熱放散反応" 日本生理学雑誌. 53(3). 112 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 李 嘉明他: "ナキウサギ(Pika)による温熱耐性の温熱耐性の温熱生理学的解析" 日本ハイパ-サ-ミア学会誌. 6(3). 343 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ohwatari,N.et al.: "Influence of local spinal heating on body temperature in the rabbit.In.Hyperthermic Oncology in Japan '89" Ed.by Masao Saito,Tokyo Univ.Press, 320-321 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsumoto,T.et al.: "Changes in circulating hormone levels induced by whole body hyperthermia in man.In.Hyperthermic Oncology in Japan '89" Ed.by Masao Saito,Tokyo Univ.press, 312-313 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsumoto,M;et al.: "Effect of fine ambient temperature on thermal sweating at rest and during exercise under thermo-neutral conditions" Trop.Med.31(3). 131-139 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohwatari,N;et al.: "Heat loss response induced by local heating of spinal cord with argon laser" Trop.Med.31(1). 147-150 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohwatari,N;et al.: "Anallysis of heat loss responses induced by local heating of thermally sensitive tissues in the CNS fo rabbits with an argon laser" Thermal Physiology. 129-134 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Matsumoto,T;et al.: "Influence of thermal stimuli on serum prolactin level in man" Thermal Physiology. 369-374 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kosaka,M;et al.: "Cerebral blood flow change due to thermal acclimation" Thermal Physiology. 463-468 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tsuchiya,K;et al.: "Effect of loose restraint on body temperature in spontaneously hypertensive rats(SHR)and stroke-prone SHR(SHRSP)" Thermal Physiology. 563-568 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohwatari,N;et al.: "Hyperthermic Oncology(321ー323)" Taylor & Francis London.New York.Philadelphia Ed.by Tsutomu Sugawara & Masao Saito, 1-920 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kosaka,M;et al.: "Hyperthermic Oncology(330ー333)" Taylor & Francis London.New York.Philadelphia Ed.by Tsutomu Sugawara & Masao Saito, 1-812 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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