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Immunological study on the mechanisms of local eosinophil infiltrations in helminthic infection.

Research Project

Project/Area Number 01480169
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionAkita University

Principal Investigator

YOSHIMURA Kentaro  Akita University, Professor, 医学部, 教授 (90053058)

Co-Investigator(Kenkyū-buntansha) SHIMADA Hiroko  Akita University, Research Associate, 医学部, 助手 (30235626)
ISHIDA Kazuto  Akita University, Research Associate, 医学部, 助手 (60006731)
TANI Shigekazu  Akita University, Research Associate, 医学部, 助手 (10006728)
ABE Tatsuya  Akita University, Associate professor, 医学部, 助教授 (80128363)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1991: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1989: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsAngiostrongylus cantonensis / eosinophil / eosinophil chemotactic factor / cerebrospinal fluid / monoclonal antibody / hypodense eosinophil / affinity chromatography / chemotactic assay / 間接蛍光抗体法 / Mesocestoides corti / ELISA / Western blotting / レセプタ- / モルモット / ラット
Research Abstract

This study deals with the possible mechanisms of local eosinophil infiltrations in Angiostrongylus cantonensis infections with special emphasis on parasite-derived eosinophil chemotactic factors and their receptors. The results obtained are sunearized as follows : Guinea-pig-eosinophils possess receptor (s) against eosinophil chemotactic factor (s) (ECF) derived from the young adult wores of A. cantonensis (YA), whereas rateosinophils are devoid of the receptor or, if any, the numbers of the receptor are few. The receptor is proteinaceous and reproducible. Hybridomas prepared from spleen cells of mice igmunized with partially Purified ECF-YA produced 2 kinds of sonoclonal antibodies (mabs) recognizing either 16.1 kD or 85 kD component in YA-whole wore extract. These mAbs were IgGl isotype. Both mAbs werespecifically reactive to ECF-YA, but failed to react to the respective whole worm extracts of A. cantonensis first stage larvae, Metastrangylus apri, Fasciola sp. and Spirometra erinace … More i plerocercoids. Several mAbs against 16.1 kD and 85 kD components all recognized the same corresponding epitopes on 16.1 kD and 85 kD antigens. Both anti-16.1 kD and anti-85 kD mAbs were capable of inhibiting eosinophil chemotactic activity of YA-whole worm extract but the former mAb gave a stronger inhibitory effect. The combination of both mAbs showed a stronger inhibitory effect than either mAb alone. Neither mAbs inhibited the chemotactic activity of ECFs derived from other helminth parasites. An affinity chromatography with anti-16.1 kD mAb succeeded in isolating a 16.1 kD antigen from YA-whole worm extracts. The ECF-YA was localized in the intestinal cytoplasm of the young adult worms. The ECF-YA can be discharged from the worms as an excretory and secretory product. Eosinophils in the cerebrospinal fluid (CSF) of mice infected with A. cantonensis were hypodense, while blood eosinophils from the same animals were normodense. Blood eosinophilia induced by the previous infection with other helminth parasite (Mesocestoides corti) failed to lead to CSF eosinophilia in subsequent A. cantonensis infection. CSF eosinophilia in A. cantonensis infection is probably provoked by ECF-YA and/or cytokines produced by lymphocytes sensitized with A. cantonensis antigens. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Yoshimura,Kentaro: "Protective immunity Against angiostrongylus cantonensis in rats following sensitizing infections" Parasitol.Res.76. 26-31 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishida,Kazuto: "Differences in responses of rat-and guinea-pig-eosinophils to eosinophil chemotactic factors derived from Angiostrongylus cantonensis" Parasite Immunol.12. 269-283 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hua,Xianxin: "Alteration in density of eosinophils in the cerebrospinal fluid of mice infected with Angiostrongylus cantonensis" Int.J.Parasitol.20. 681-683 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishida,Kazuto: "Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis" Parasite Immunol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshimura,Kentaro: "The influence of Mesocestoides corti on subsequent Angiostrongylus cantonensis infections in mice" Int.J.Parasitol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshimura,Kentaro: "The eosinophil in parasitic infections.In "Current Concepts in Parasitology"edited by R.C.Ko.,pp.7-46" Hong Kong University Press, 267 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 吉村 堅太郎: "寄生虫の宿主特異性と好酸球ー広東住血線虫をモデルとしてー(寄生虫症モデル).牧野荘平・石川哮編“好酸球",pp.136ー146" 国際医学出版,東京, 282 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshimura, K., Ishida, K., Ishigooka, S., Sugaya, H. & Kumagai, M.: "Protective immunity against Angiostrongylus cantonensis in rats following sensitizing infections" Parasitol. Res.76. 26-31 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishida, K. & Yoshimura, K.: "Differences in responses of rat and guinea-pig-eosinophils to eosinophil chemotactic factors derived from Angiostrongylus cantonensis" Parasite Immunol.12. 269-283 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hua, X., Sugaya, H. & Yoshimura, K.: "Alteration in density of eosinophils in the cerebrospinal fluid of mice infected with Angiostrongylus cantonensis" Int. J. Parasitol.20. 681-683 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ishida, K. & Yoshimura, K.: "Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis" Parasite Immunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshimura, K., Sugaya, H. & Ishigooka, S.: "The influence of Mesocestoides corti on subsequent Angiostrongylus cantonensis infections in mice" Int. J. Parasitol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshimura, K.: "The eosinophil in parasitic infections." "Current Concepts in Parasitology". Hong Kong University Press. 7-46 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshimura, K.: "Host-specificity of parasites and eosinophils, with special reference to Angiostrongylus cantonensis. A parasitic disease model." "Eosinophils". Int. Med. Publishers. 136-146 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshimura,Kentaro: "Role of eosinophils in Angiostrongylus cantonensis infection" Ann.Parasitol.Hum.Comp.66(Suppl.I). 42-43 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ishida,Kazuto: "Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis" Parasite Immunol.

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshimura,Kentaro: "The influence of Mesocestoides corti on subsequent Angiostrongylus cantonensis infections in mice" Int.J.Parasitol.

    • Related Report
      1991 Annual Research Report
  • [Publications] 吉村 堅太郎: "寄生虫の宿主特異性と好酸球ー広東住血線虫をモデルとしてー〈寄生虫症モデル〉.牧野荘平・石川哮編“好酸球",pp.136ー146" 国際医学出版,東京, 282 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ishida,Kazuto: "Differences in responses of ratー and guineaーpig eosinophils to eosinophil chemotactic factors derived from <Angiostrongylus>___ー <cantonensis>___ー." Parasite Immunology. 12. 269-283 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hua,Xianxin: "Alteration in density of eosinophils in the cerebrospinal fluid of mice infected with <Angiostrongylus>___ー <cantonensis>___ー." International Journal for Parasitology. 20. 681-683 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 吉村 堅太郎: "寄生虫の感染防御における好酸球の役割" 臨床免疫. 22. 772-782 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 吉村 堅太郎: "なぜ寄生蠕虫感染症では好酸球が増えるか、また好酸球の役割は何か" Medical Technology. 18. 188-189 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshimura,Kentaro: "Protective immunity against Angiostrongylus cantonensis in rats following sensitizing infections" Parasitology Research. 76. 26-31 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ishida,Kazuto: "Differences in responses of rat-and guinea-pig-eosinophils to eosinophil chemotactic factors derived from Angiostronglus cantonensis" Parasite Immunology. 12. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hua,Xianxin: "Density alteration of eosinophils in the cerebrospinal fluid of mice infected with Angiostrongylus cantonensis" International Journal for Parasitology. 20. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshimura,Kentaro: "The eosinophil in parasitic infections.In“Current Concepts in Parasitology"edited by R.C.Ko.,pp.7-46." Hong Kong University Press, 267 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2021-09-24  

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