• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Immuno-Melecular characterization of lymphocyte functioning antigen (LFA)

Research Project

Project/Area Number 01480194
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionJuntendo University School of Medicine

Principal Investigator

OKUMURA Ko  Juntendo University, Professor, 医学部, 教授 (50009700)

Co-Investigator(Kenkyū-buntansha) OBATA Tetsuji  Juntendo University, Associate Professor, 医学部, 講師 (10205445)
YAGITA Hideo  Juntendo University, Associate Professor, 医学部, 助教授 (30182306)
屋部 登志雄  順天堂大学, 医学部, 助手 (50239836)
中村 哲也  順天堂大学, 医学部, 助手 (30189047)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥5,700,000 (Direct Cost: ¥5,700,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1990: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1989: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsLFA / VLA / mAb / lymphocyte / tolerance / lymphocyte / CD2 / TcR / トランスフェクタント / CD4
Research Abstract

Cell interactions in the immune system are mediated by several different families of receptors on cell surface, and these adhesion molecules influence many diverse processes including, differentiation, migration and cellular activation. To understand in vivo significance of these molecules in various and immunological phenomenon, we have been mainly, focussing murine as well as rat experimental systems. We report the experimental results performed to understand the role of VLA-4, LFA-1, and ICAM-1 in cell migration and activation in rat and mouse experimental systems.
(1) Anti-rat VLA-4 mAb, which we established, could inhibit lymphocyte homing to Payeris patch selectively.
(2) Anti-rat VLA-4- mAb inhibited the infiltration of lymphocytes, monocytes and eosinophils but not neutrophils to inflamatory sites.
(3) Fibronectin acted costimulatory in FceRI-mediated activation of rat mast cells. This costimlatory effect was mainly mediated by VLA-4.
(4) Anti-VLA-4 mAb prevented the cardiac allograft rejection more strongly than anti-LFA-1 mAb.
(5) Mixed administration of inti-LFA-1 and anti-ICAM-1 mAbs caused specific cardiac allograft tolerance in both murine and rat systems.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Nakamura,T.,Takahashi,K.,Koyanagi,M.,Y.,et al.: "Acivation of a natural killer clonc upon target cell binding via CD2." Eur.J.Immunol.21. 831-834 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ota,Y.,Kobata,T.,Seki,M.,et al.: "Exterathymic origin of Vγ1/Vδ6 T cells in the skin" Eur.J.Immunol.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iigo,Y.,Takashi,T.,Tamatani,T.,et al.: "ICAM-1-dependent pathway is critically involved in the pathogenesis of adjuvant arthritis in rats." J.Immunol.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Isobe,M.,Ihara,A.,Okumura,et al.: "A short course of anti-ICAM/anti-LFA-1 treatment leads to specific acceptance of cardiac allograft." Science. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takahashi,K.,Nakata,M.,Tanaka,T.,et al.: "CD4 and CD8 regulate IL-2 response of T cells:Involvement of P56^<1ck>." Proc.Natl.Acad.Sci.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura, T., Takahashi, K., Koyanagi, M., Y., et al.: "Activatin of a natural killer clone upon target cell binding via CD2." Eur. J. Immunol.21. 831-834 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ota, Y., Kobata, T., Seki, M., et al: "Exterathymic origin of V_<gamma>1/V_<delta>6 T cells in the skin" Eur. J. Immunol.,. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iigo, Y., Takashi, T., Tamatani, T., et al.: "ICAM-1-dependent pathway is critically involved in the pathogenesis of adjuvant arthritis in rats." J. Immunol.,. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Isobe. M., Ihara, A., Okumura, et al.: "A short course of anti-ICAM/anti-LFA-1 treatment leads to specific acceptance of cardiac allograft." Science. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takahashi, K., Nakata, M., Tanaka, T., et al.: "CD4 an CD8 regulate IL-2 response of T cells : Involvement of p56^<1ck>." Proc. Natl. Acad. Sci.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakamura,T.,Takahashi,K.,Koyanagi,M.,Y.,et al.: "Activation of a natural killer clone upon targer cell binding via CD2." Eur.J.Immunol.21. 831-834 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ota,Y.,Kobata,T.,Seki,M.,et al.: "Exterathymic origin of Vγ1/Vδ6 T cells in the skin" Eur.J.Immunol.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iigo,Y.,Takashi,T.,Tamatani,T.,et al.: "ICAMー1ーdependent pathway is critically involved in the pathogenesis of adjuvant arthritis in rats." J.Immunol.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Isobe,M.,Ihara,A.,Okumura,et al.: "A short course of antiーICAM/antiーLFAー1 treatment leads to specific acceptance of cardiac allograft." Science. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takahashi,K.,Nakata,M.,Tanaka,T.,et al.: "CD4 and CD8 regulate ILー2 response of T cells:Involvement of p56^<1ck>." Proc.Natl.Acad.Sci.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tetsuji Kobata: "Thyー1 positive dendritic epidermal cells contain a killer protein perforin." Int.Immunol.2. 1113-1116 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tetsuya Nakamura: "Relative contribution of CD2 and LFAー1 to murine T and Natural Killer cell functions." J.Immunol.145. 3628-3634 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kazuhisa Takahashi: "A murine very late activation antigenーlike extracellular matrix reseptor involved in CD2^- and lymphocyte functionassociated antigenー1ーindependent killirーtarget cell interaction." J.Immunol.145. 4371-4379 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Motomi Nakata: "Constitutive expression of poreーforming protein in perpheral blood γ/δ Tcells:Implication for their cytotoxic role in vivo." J.Exp.Med.172. 1877-1880 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tetsuya Nakamura: "Activation of a NK clone upon target cell binding via CD2." Eur,J.Immunol.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kazuhisa Takahashi: "Antigenーindependent T cell activation mediated by a VLAーlike extracellular matrix receptor" Eur,J.Immunol.

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideo Yagita: "Monoclonal antibodies specific for murine CD2 reveal its presence on B as well as T cells." Proc.Natl.Acad.Sci.USA. 86. 645-649 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hideo Yagita: "Activation of peripheral blood T cells via the p75 interleukinー2 receptor." J.Exp.Med.170. 1445-1450 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Taizo Nitta: "Bispecific F(ab')_2monomer prepared with antiーCD3 and antiーtumor monoclonal ant ibodies is most potent in induction of cytolysis of human T cells." Eur,J.Immunol.19. 1437-1441 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Taizo Nitta: "Involvement of CD56(NKHー1/Leu19 antigen)as an adhesion molecule in natural Killarーtarget Cell interaction." J.Exp.Med.170. 1757-1761 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hitoshi Ishikawa: "Molecular Cloning of rat cytolysin" J.Immunol.143. 3069-3073 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoichi Shinkai: "Molecular cloning and chromosomal assingment of a human perforin(PFP)gene." Immunogenetics. 30. 452-457 (1989)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi