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Preclinical Study of Radioimmunoguided Surgery Using Metastases Model of Human Colon Cancer in Congenitally Athymic mice.

Research Project

Project/Area Number 01480334
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionThe Jikei University School of Medicine

Principal Investigator

SAKURAI Kenji  The Jikei Univ. School of Med. 1st Dept. of Surgery, Professor, 医学部・第一外科, 教授 (00147315)

Co-Investigator(Kenkyū-buntansha) UNEMURA Yasuki  The Jikei Univ. School of Med. 1st Dept. of Surgery, Assistant, 第一外科, 助手 (00193920)
YOSHIDA Kazuhiko  The Jikei Univ. School of Med. 1st Dept. of Surgery, Assistant, 第一外科, 助手 (90191577)
ISHIDA Shusei  The Jikei Univ. School of Med. 1st Dept. of Surgery, Assistant, 第一外科, 助手 (60168226)
蜂谷 公敏  東京慈恵会医科大学, 第一外科, 助手 (80180892)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsMonoclonal antibody / Radioimmunoguided surgery / Colon cancer / Hepatic metastases / Tumor specific antigen / radioimmunoguided surgery
Research Abstract

Reproducible murine metastases models in the liver, lung, and peritoneum from human colon cancer (HCC) were developed in congenitally athymic mice, and the utility of a gamma detecting probe (GDP) in the detection of hepatic metastases using radiolabeled monoclonal antibody (mAb) was assessed.
One hundred percent of mice developed hepatic metastases after intrasplenic injection of HT29LMM HCC cells, and 100% also had peritoneal metastases after intraperitoneal injection. Additionally, 60% of mice developed lung metastases after tail vein injection.
Mice with hepatic metastases were injected intravenously with 40 mu Ci/4mugof I-125 labeled mAb HT 29-15 and an isotype-matched control mAb (BL-3). And non-tumor-bearing control mice were injected with the same dose of I-125 labeled mAb HT29-15. Using GDP, measurements were obtained daily over the region of the heart, the region of the liver (the right flank). External right flank/heart ratios were significantly higher in the tumor-bearing group with mAb HT-29-15 than in controls. External measurements allowed detection of small tumors weighing only 161mg. Intraoperative measurements of small isolated metastasis were obtained at laparotomy on days 5 and 7. The mean metastasis/heart GDP ratio count was 1.7, and tumors as small as 51mg in weight could be identified.
These experimental results confirm the usefulness of the GDP for the detection of small hepatic metastases from colon cancer, and illustrate important features of probe measurement of radiolabeled mAb uptake.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 吉田 和彦: "大腸癌転移に関する最近の話題" 外科. 51. 914-919 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuhiko Yoshida: "A hepatic metastasis model of human colon cancer in congenitally athymic mice." Jikeikai Medical Journal. 36. 209-217 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuhiko Yoshida: "Current topics of hepatic metastases from colon cancer." Geka. 51 (9). 914-919 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kazuhiko Yoshida: "A hepatic metastasis model of human colon cancer in congenitally athymic mice." Jikeikai Medical Journal. 36. 209-217 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 吉田 和彦: "大腸癌転移に関する最近の話題" 外科. 51. 914-919 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kazuhiko Yoshida: "A hepatic metastasis model of human colon cancer in congenitally athymic mice." Jikeikai Medical Journal. 36. 209-217 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kazuhiko Yoshida: "A hepatic metastasis model of human colon cancer in congenitally athymic mice." Jikeikai Medical Journal. 36. 209-217 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 吉田和彦: "大腸癌転移に関する最近の話題" 外科. 51. 914-919 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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