AMEMIYA Toshifumi Chiba Institute of Technology, Prof., 工学部, 教授 (50023221)
MATSUI Masaaki Nagoya University School of Technology, Prof., 工学部, 教授 (90013531)
KUTIWAKI Hirozi Nagoya University School of Medicine. Assist. Prof., 医学部, 講師 (60135340)
|Budget Amount *help
¥6,600,000 (Direct Cost : ¥6,600,000)
Fiscal Year 1991 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1990 : ¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1989 : ¥3,800,000 (Direct Cost : ¥3,800,000)
l. Development of Implant Heating System (IHS)
We observed that the Fe-Pt afloy (Fe : 73%i Pt : 27%, -Curie point : 68) is the best implant needle of the Implant Heating System (IHS). And we developed IHS for clinical study. The IHS consists of three parts : implant needle, induction coil and generator. The implant needle is 1.8mm in diameter and 15, 20, 25 mm in length, of which Curie pointa is set at 68. The induction coil is 30 cm in diameter, maximum magnetic field strength at the center of the coil is 16.7 Oe. And the generator. has 2.5 kill of maximum power, yielding the high frequency of 240 kHz.
2. Experimental studies
We have investigated intracellular translocation of hsp-70 during at moderate temperatures in HeLa cells. When cells were heated at temperatures above 41, hsp-70 translocated from the cytoplasm into the nuclei and apparently accumulated in nucleoli within, 10 min. At temperatures above 42, hsp-70 remained in the nuclei during, heating.
Then we studied the histopathol
ogical changes in rabbit tongue tumor model (VX-7 carcinoma) after single treatment of hyperthermia using IHS. The tumor tissue at 5mm apart from implant needle was showed coagulation necrosis. The necrotic lesion -was irregular shaped and tended to extend gradually toward the peripheral side of the tongue for 3 days.
3. Clinical studies
Safe, repeated and longterm treatment of 25 cases of inoperable, recurrent brain tumors (Glioma : 13cases, Meta. : 7cases, Others : 5cases) have been made by the IHS. Before inducing hyperthermia, one or more implants were placed within the tumor by CT guided stereotaxis or inserted manually at craniotomy depending on the size of tumors. All the cases received repeated hyperthermia, combined with either radiotherapy or chemotherapy. The clinical response were 3 CR, 5 PR, 4 MR, 3 ST and 8 PD.
Thirteen cases (tonque : 7cases, mouth floor : 2cases, gingiva : 2cases, palate : lcase lip : lcase) of oral cancer has been treated by IHS. Three cases were treated by IHS, irradiation and chemotherapy, and three cases were treated by IHS and chemotherapy (CDDP : 200mg, PEP : 70-80mg). The other 7 cases were treated by IHS, chemotherapy and operation. The clinical response were 9 CR and 4 PR. Less