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Analysis of Schistosoma Japonicum Egg Antigen Epitopes Inducing T Lymphocyte Response in Humans.

Research Project

Project/Area Number 01570214
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionOkayama University

Principal Investigator

OHTA Nobuo  Okayama Univ Med School, Dept of Parasitol, Assoc Prof., 医学部, 助教授 (10143611)

Co-Investigator(Kenkyū-buntansha) AJI Toshiki  Okayama Univ Med School, Dept of Parasitol, Lecturer., 医学部, 講師 (10032905)
ISHI Akira  Okayama Univ Med School, Dept of Parasitol, Prof., 医学部, 教授 (40012752)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsSchistosoma japonicum / Delayed hypersensitivity / Cytokine / T cell clones / T-western method / Suppressor T cells / Idiotype / Tウェスタン / クロ-ン化T細胞 / T細胞ウエスタン法
Research Abstract

We analyzed Schistosoma japonicum egg antigen molecules and epitopes which induced pathologically important T cell responses by the method of T-western. We isolated human T cell lines and clones (CD3+CD4+CD8-) from Japanese patients with chronic schistosomiasis japonica. Those T cells showed significant proliferation as well as cytokine production such as interleukin-2 and gamma-interferon under the stimulation of S. japonicum egg antigen in vitro. It was, thus, suggested that those T cells were involved in circum-oval granuloma formation in vivo. We tested those T cells in T-western experiments to identify the egg antigen molecules which induced and modulated T cell response in humans. Since the original T-western method of Young & Lamb had some defects, we applied an improved T-western method reported by Abou-Zeid et al. This gave improved sensitivity and reproducibility in evaluating T cell response. We observed that at least four egg antigen molecules, of which molecular weight were 16kD, 33kD, 48kD and 113kD, were likely to be main targets for pathological T cell responses in man. Of four egg antigen molecules, 33kD and 48kD molecules seemed to be important in immunomodulation because suppressor T cells recognized those two egg antigen molecules. Detailed epitope analysis is still underway ; however, we obtained data suggesting that epitopes on the 16kD molecule (s) were somehow related to IgG idiotypes expressed in human infected sera.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Ohta N.: "Disappearance of specific antibodies in patients with chronic schistosomiasis japonica by treatment with praziquantel." Japanese Journal of Medical Science and Biology. 42. 31-38 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki T.: "HLAーlinked immune suppression in humans." Immunology. supple 2. 21-25 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 太田 伸生: "日本住血吸虫症におけるT細胞応答の制御機構" 医学のあゆみ. 151. 207 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "HLAーDQーcontrolled T cell respones to soluble egg antigen of Schistosoma japonicum in humans." Clinical and Experimental Immunology. 79. 403-408 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 太田 伸生: "ヒト住血吸虫症と免疫応答" 感染・炎症・免疫. 20. 25-38 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "Regulation of the human T cell response to Schistosoma japonicum egg antigen by concomitant cellular and humoral mechanisms in vitro." Parasitology Research. 77. 54-58 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 太田 伸生: "HLAと感染" Medical Immunology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 太田 伸生: "日本住血吸虫症の免疫病理学的研究:宿主T細胞の応答制御を通じた病因解析および寄生虫による宿主の発がん機構について" 岡山医学会雑誌. 103. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "Comparison of T and B lymphocyteーrecognition molecules of Schistosoma japonicum egg antigen in humans."

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "Disappearance of specific antibodies in patients chronic schistosomiasis japonica by treatment with praziquantel." Japanese Journal of Medical Science and Biology. 42. 31-38 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sasazuki T.: "HLA-linked immune suppression in humans." Immunology. Supple 2. 21-25 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "Regulatory mechanisms of T cell response in schistosomiasis japonica. (Text in Japanese)" Igakunoayumi. 151. 207 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "HLA-DQ-controlled T cell response to soluble egg antigen of Schistosoma japonicum in humans." Clinical and Experimental Immunology. 79. 403-408 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "Immunoregulation during human schistosomiasis. (Text in Japanese)" Infection, Inflammation and Immunity. 20. 25-38 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "Regulation of the human T cell response to Schistosoma japonicum egg antigen by concomitant cellular and humoral mechanisms in vitro." Parasitology Research. 77. 54-58 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "HLA and infectious diseases. (Text in Japanese)" Medical Immunology. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "Immunopathology of schistosomiasis japonica : Analysis of the immunopathogenesis through regulatory mechanisms of host T cell response and carcinogenesis induced by the parasites." Okayama Igakkai Zasshi,. 103. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.: "Comparison of T and B lymphocyte-recognition molecules of Schistosoma japonicum egg antigen in humans."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohta N.,Edahiro T.,Ishii A.,Yasukawa M & Hosaka Y.: "HLAーDQーcontrolled T cell response to soluble egg antigen of Schistosoma japonicum in humans." Clinical and Experimental Immunology. 79. 403-408 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 太田 伸生: "ヒト住血吸虫症と免疫応答" 感染・炎症・免疫. 20. 25-38 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ota N.,Asahi H.,Hosaka Y.,Minai M & Ishii A.: "Regulation of human T cell response to Schistosoma japonicum egg antigen by concomitant cellular and humoral mechanisms in vitro." Parasitology Research. 77. 54-58 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 太田 伸生: "HLAと感染" Medical Immunology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 太田 伸生,安治 敏樹,松岡 裕之,東儀 宣哲,小野 俊朗,新井 明治,枝廣 徹,石井 明: "日本住血吸虫症の免疫病理学的究:宿主T細胞の応答制御を通じた病因解析および寄生虫による宿主の発がん機構について" 岡山医学会雑誌. 103. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ohta N.,Song J & Ishii A.: "Comparison of T and B lymphocyteーrecognition molecules of Schistosoma japonicum egg antigen in humans."

    • Related Report
      1990 Annual Research Report
  • [Publications] Ohta、N.,Hosaka,Y.,Minai,M.,Hayashi,M.& Miki,Y.: "Disappearance of specific antibodies in patients with chronic schistosomiasis japonica by treatment with rpaziquantel" Japanese Journal of Medical Science and Biology. 42. 31-38 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sasazuki,T.,Kikuchi,I.,Hirayama,K.,Matsushita,S.,Ohta,N.& Nishimura,Y.: "HLAーlinked immune suppression in humans." Immunology. Supple 2. 21-25 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 太田伸生: "日本住血吸虫症におけるT細胞応答の制御機構" 医学のあゆみ. 151. 207 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohta,N.,Edahiro,T.,Ishii,A.,Yasukawa,M.,Hosaka,Y.: "HLAーDQーcontrolled T cell response to soluble egg antigen of Schistosoma japonicum in humans." Clinical and Experimental Immunology. 79. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 太田伸生: "ヒト住血吸虫症と免疫応答" 感染・炎症・免疫. 20. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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