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Effect of Environmental Factors on the Mutagenicity of Human Urine

Research Project

Project/Area Number 01570289
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Hygiene
Research InstitutionKyushu University

Principal Investigator

INAMASU Takeo  Kyushu University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (70127976)

Co-Investigator(Kenkyū-buntansha) ISHINISHI Noburu  Kyushu University, Faculty, of Medicine, Professor, 医学部, 教授 (80037340)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsAmes test / mutagenicity / human urine / the aged person / pregnant woman / 尿 / Ames test
Research Abstract

Mutagenicity of spot urine from male aged persons (60 years old<) and pregnant women (20 years old< >40 years old) were examined by Ames test using Salmonella typhimurium TA98 in the presence of S-9 mix. As control groups, male adolescents (20 years old< >35 years old) and non-pregnant females (20 years old<>40years old) were chosen respectively. Mutagens in the urine samples were adsorbed on the XAD-2 resin column and eluted by dichloromethane (DCM) and acetone consecutively. Each fractions were tested separately for the mutagenicity. The sum of mutagenicities obtained from each fractions was expressed as total mutagenity.
The mean <plus-minus> S. D. of total mutagenicity of urine were 124 <plus-minus> 73 and 119 <plus-minus> 53 revertants per 100 ml urine for 22 aged nonsmokers and 53 male adolescent nonsmokers, respectively. The difference between the two groups were not significant. The ratio of mutagenicity from DCM to the total mutagenicity for the aged nonsmokers tended to be low … More er than those of adolescent nonsmokers, smokers and aged smokers. Creatinine contents in the aged persons' urine were significantly lower than those in the adolescent urine. This suggests that the kidney function of the aged persons was somewhat da
The mean <plus-minus> S. D. of total mutagenicity of nonsmokers urine were 98 <plus-minus> 55 and 68 <plus-minus> 61 revertants per 100 ml urine for 28 pregnant nonsmokers and 12 non・pregnant women, respectively. The difference between the two groups were not significant. Combined effects of DCM and acetone fractions from pregnant and non・pregnant females was also examined individually and mostly showed inhibitory effect, compared with the total mutagenicity of each individuals. The addition of acetone fraction from pregnant and non-pregnant women to benzo (a) pyrene (B (a) P) suppressed the mutagenicity of the B (a) P to the degree of 58-59% of the B (a) P mutagenicity. These results suggests that antimutagenic substances may be contained in the Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 稲益 建夫: "高齢者及び妊婦の尿の変異原性について" 日本衛生学会.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takeo Inamasu: "Mutagenicity of urine from aged persons and pregnant women" Mutation Research.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 稲益 建夫: "高齢者及び妊婦の尿の変異原性について" 日本衛生学会. (1992)

    • Related Report
      1990 Annual Research Report
  • [Publications] 稲益建夫: "人の尿の変異原性と環境要因" 病態生理. 9. 71-73 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takeo Inamasu: "Comparison of urinary mutagenicity in young and elderly Japanese" Mutation Research.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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