Effect of Environmental Factors on the Mutagenicity of Human Urine

Research Project

Project/Area Number	01570289
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	Hygiene
Research Institution	Kyushu University
Principal Investigator	INAMASU Takeo Kyushu University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (70127976)
Co-Investigator(Kenkyū-buntansha)	ISHINISHI Noburu Kyushu University, Faculty, of Medicine, Professor, 医学部, 教授 (80037340)
Project Period (FY)	1989 – 1990
Project Status	Completed (Fiscal Year 1990)
Budget Amount *help	¥2,100,000 (Direct Cost: ¥2,100,000) Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000) Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywords	Ames test / mutagenicity / human urine / the aged person / pregnant woman / 尿 / Ames test
Research Abstract	Mutagenicity of spot urine from male aged persons (60 years old<) and pregnant women (20 years old< >40 years old) were examined by Ames test using Salmonella typhimurium TA98 in the presence of S-9 mix. As control groups, male adolescents (20 years old< >35 years old) and non-pregnant females (20 years old<>40years old) were chosen respectively. Mutagens in the urine samples were adsorbed on the XAD-2 resin column and eluted by dichloromethane (DCM) and acetone consecutively. Each fractions were tested separately for the mutagenicity. The sum of mutagenicities obtained from each fractions was expressed as total mutagenity. The mean <plus-minus> S. D. of total mutagenicity of urine were 124 <plus-minus> 73 and 119 <plus-minus> 53 revertants per 100 ml urine for 22 aged nonsmokers and 53 male adolescent nonsmokers, respectively. The difference between the two groups were not significant. The ratio of mutagenicity from DCM to the total mutagenicity for the aged nonsmokers tended to be low … More