HPLC Analysis of Stereoisomers of Phenethylamine and its Application to Forensic Toxicology.
Project/Area Number |
01570342
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Research Category |
Grant-in-Aid for General Scientific Research (C)
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Allocation Type | Single-year Grants |
Research Field |
Legal medicine
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Research Institution | Dokkyo University School of Medicine. |
Principal Investigator |
NAGAI Toshiaki Dokkyo Univ. Sch. of Med, Med, Assistant., 医学部, 助手 (50124996)
|
Project Period (FY) |
1989 – 1990
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Project Status |
Completed (Fiscal Year 1990)
|
Budget Amount *help |
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
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Keywords | Forensic Toxicology / Methamphetamines / Optical isomer / HPLC analysis / Rat and human / Stereoselective metabolism / Urine / 法医学的応用 / ラット / ヒト / 立体選択性代謝 / Amphetamine / 尿 / 高速液体クロマトグラフィ- |
Research Abstract |
Simultaneous identification of optical isomers (d and 1) of methamphetamine (MAMP), amphetamine (AMP), para (p) -hydroxy (OH) -MAMP and para (p) -hydroxy (OH) -AMP in rat urine was attempted by high-performance liquid chromatography (HPLC). They were determined as Benzoyl derivatives, After administration of d- or 1-isomer (15mg/kg), only d- or 1-isomers of the above mentioned metabolites were found in rat urine. In rats administered racemic MAMP (15mg/kg), each percent dose of 1-isomers of 1-isomers of MAMP or AMP excreted at four collection time up to 24 hours was less than that of the d-isomer (1/d 1.00), but the doses of 1-isomers of p-OH-MAMP and p-OH-AMP excreted were higher than those of d-isomers (1/d 1.00). Simultaneous analysis within 36 minutes showed good peak resolution. The 1/d ratio for each metabolite decreased with time. The total percent doses of d- or 1-isomer excreted by 24 hours were about 50% of the administered dose, 23.70<plus-minus>1.45% for the d-isomer, 25.70<plus-minus>1.54% for the 1-isomers. The total 1/d ratio was 1.08<plus-minus>0.03. These results indicated that Wistar rats have no chiral isomerization enzyme of MAMP, and show stereoselective metabolism of dl In the optical isomer analysis of ll MAMP powder samples and 28 human urine specimens obtained from japanese abusers, only d-MAMP was detected from the powder, and d-MAMP and d-AMP were done from urine, respectively. This suggested that humans have no chiral isomerization enzyme of d-MAMP.
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Report
(3 results)
Research Products
(6 results)