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Role of anti-DNA antibody and anti-cardiolipin antibody for the progression of vasculopathy in collagen diseases

Research Project

Project/Area Number 01570348
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionChiba University

Principal Investigator

KOIKE Takao  Chiba University School of Medicine, Assistant, 医学部・第二内科, 助手 (80146795)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordssystemic lupus erythematosus / anti-phospholipid antibody / anti-cardiolipin antibody / anti-DNA antibody / lupus nephritis / thrombosis / fetal loss / V region gene / 血管病変 / 流・早産 / ハイブリド-マ
Research Abstract

To clarify the specificity of anticardiolipin antibody (aCL), we developed 18 monoclonal hybridoma aCL from SLE prone, MRL-1pr/1pr mice and the reactivity of these antibodies to phospholipids, DNA, epithelial cells (HEp-2 cells), platelets, endothelial cells, heparin, protein C and thrombomodulin was examined. All the monoclonal aCL reacted with phosphatidylserine and 11 of 18 also reacted with phosphatidylinositol. Three of 18 hybridoma aCL bound to both ssDNA and dsDNA, and 10 of 18 bound to ssDNA but not to dsDNA. The characteristics of antinuclear antibody were manifest for 10 of 18 monoconal aCL reacted with platelets. Three of 18 monoclonal aCL bound to vascular endothelial cells and these monoclonal aCL also had a strong reactivity to heparin. No monoclonal aCL showed reactivity to protein C or to thrombomodulin.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Takabayashi,K.,Koike,T.Kurasawa,K.,Matsumura,R.,Sato,T.,Tomioka,H.,Ito,I.,Yoshiki,T.,Yoshida,S.: "Effect of FKー506,a novel immunosuppressive drug on muine SLE." Clin.Immunopathol.51. 110-117 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimada,K.,Koike,T.,Ichikawa,K.,Tsutsumi,A.,Takabayashi,K.,Tomioka,H.,Yoshida,S.: "IgG class antiーcardiolipin antibody as a possible marker for evaluating fetal risk in patients with systemic lupis erythematosus" J.Autommunity. 2. 843-849 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shigemoto,K.,Maruyama,N.,Itoh,Y.,Kubo,S.,Koike,T.,: "Murine Ieukemia virus gene product as an acute phase protein;Complete suppression of serum gp70 synthersis in hepatocytes of B10.S mice." Clin.Exp.Immunol.78. 484-487 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kurasawa,K.,Koike,T.,Matsumura,R.,Takabayashi,K.,Tomioka,H.,Ito,I.,Yoshida,S.: "The immunosuppressive FKー506 prevents progression of diabetes in in NOD mice." Clin.Immunol.Immunopathol.57. 274-279 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiruma,K.,Kioke,T.,Nakamura,H.,Sato,T.,Maeda,T.,Tomioka,H.,Yoshida,S.,Fujita,T.: "Somatostatin receptors on human lymphocytes and leukemic cells" Immunology. 71. 480-485 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsuura,E.,Igarashi,Y.,Fujimoto,M.,Ichikawa,K.,Koike,T.: "Anticardiolipin cofactor(s) and differntial diagnosis of autoimmune disease" Lancet. 336. 177-178 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小池 隆夫: "中外医学社" Annual Review 免疫,1989, 295 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 小池 隆夫: "中外医学社" Annual Review 免疫,1990, 382 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsutsumi, A., Koike, T., Nawata, Y., Yoshida, S., Tomioka, H.: "IgG subclass of anti-IgE autoantibody in allergic disorders." Immunol. Allergy Practice. 10. 149-154 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ichikawa, K., Shimada, K., Nawata, Y., Ishii, T., Tomioka, H., Yoshida, S., Koike, T.: "Monoclonal hybridoma anti-cardiolipin antibodies from SLE mice" Clin. Exp. Immunol.74. 110-114 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsutsumi, A., Koike, T., Ichikawa, K., Shimada, K., Takabayashi, K., Tomioka, H., Yoshida, S.,: "IgG subclass distribution of anti-cardiolipin antibody in patients with systemic lupus erythematosus" J. Rheumatol.15. 1764-1767 (198)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsumura, R., Kondo, Y., Sugiyama, T., Sueishi, M., Koike, T., Tomioka, H., Yoshida, S.: "Immunohistochemical identification of infiltrating mononuclear cells in tubulointerstitial nephritis associated with Sjogren's syndrome." Clin. Nephrol.30. 335-340 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takabayashi, K., Koike, T., Kurasawa, K., Matsumura, R., Sato, T., Tomioka, H., Ito, I., Yoshiki, T., Yoshida, S: "Effect of FK-506, a novel immunosuppressive drug on murine SLE." Clin. Immunol. Immunopathol.51. 110-117 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimada, K., Koike, T., Ichikawa, K., Tsutsumi, A., Takabayashi, K., Tomioka, H., Yoshida, S.: "IgG class anti-cardiolipin antibody as a possible marker for evaluating fetal risk in patients with systemic lupus erythematosus." J. Autoimmunity. 2. 843-849 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shigemoto, K., Maruyama, N., Itoh, Y., Kubo, S., Koike, T.: "Murine leukemia virus gene product as an acute phase protein : Complete suppression of serum gp70 synthesis in hepatocytes of B10. S mice." Clin. Exp. Immunol.78. 484-487 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kurasawa, K., Koike, T., Matsumura, R., Takabayashi, K., Tomioka, H., Ito, I., Yoshida, S.: "FK-506 prevents progression of diabetes in NOD mice." Clin. Immunol. Immunopathol.57. 274-279 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiruma, K., Koike, T., Nakamura, H., Sato, T., Maeda, T., Tomioka, H., Yoshida, S., Fujita, T.: "Somatostatin receptors on human lymphocytes and leukemia cells." Immunology. 71. 480-485 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsuura, E., Igarashi, Y., Fujimoto, M., Ichikawa, K., Koike, T.: "Anticardiolipin cofactor(s) and differential diagnosis of autoimmune disease." Lancet. 336. 177-178 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsutsumi, A., Koike, T., Sugiyama, T., Matsumura, R., Sueishi, M., Takabayashi, K., Tomioka, H., Yoshida, S.: "Protein losing entheropathy in systemic lupus erythematosus, mixed connective tissue disease and Sjogren's syndrome." Ann. Rheum. Dis.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sumida, T., Maeda, T., Koike, T., Tomioka, H., Yoshida, S., Takemori, T., Rajewsky, K.: "Expression of IgH promoter/enhancer Ly-1 transgene in hematopoietic chimeric mice generated by embryonic stem (ES) cell line." Int. Arch. Allergy Appl. Immunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takabayashi,K.,Koike,T.Kurasawa,K.,Matsumura,R.,Sato,T.,Tomioka,H.,Ito,I.,Yoshiki,T.,Yoshida,S.: "Effect of FKー506,a novel immunosuppressive drug on murine SLE." Clin.Immunol.Immunopathol.51. 110-117 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shimada,K.,Koike,T.,Ichikawa,K.,Tsutsumi,A.,Takabayashi,K.,Tomioka,H.,Yoshida,S.: "IgG class antiーcardiolipin antibody as a possible marker for evaluating fetal risk in patients with systemic lupis erythematosus" J.Autoimmunity. 2. 843-849 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shigemoto,K.,Maruyama,N.,Itoh,Y.,Kubo,S.,Koike,T.,: "Murine leukemia virus gene product as an acute phase protein:Complete suppression of serum gp70 synthesis in hepatocytes of BlO.S mice." Clin.Exp.Immunol.78. 484-487 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kurasawa,K.,Koike,T.,Matsumura,R.,Takabayashi,K.,Tomioka,H.,Ito,I.,Yoshida,S.: "The immunosuppressive FKー506 prevents progression of diabetes in in NOD mice." Clin.Immunol.Immunopathol.57. 274-279 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiruma,K.,Kioke,T.,Nakamura,H.,Sato,T.,Maeda,T.,Tomioka,H.,Yoshida,S.,Fujita,T.: "Somatostatin receptors on human lymphocytes and leukemic cells" Immunology. 71. 480-485 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsuura,E.,Igarashi,Y.,Fujimoto,M.,Ichikawa,K.,Koike,T.: "Anticardiolipin cofactor(s)and differntial diagnosis of autoimmune disease" Lancet. 336. 177-178 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小池 隆夫: "Annual Review 免疫,1989" 中外医学社, 295 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小池 隆夫: "Annual Review 免疫,1990" 中外医学社, 382 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ichikawa,K: "Monoclonal hybridoma anti-cordiolipin antibodies from SLE mice" Clin.Exp.Immunol.74. 110-114 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tsutsumi,A: "IgG subclass distribution of anti-cardiolipin antibody in patients with systemic lupus enythematosus" J.Rheumatol.15. 1764-1767 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shimada,K.: "IgG class anti-cardiolipin antibody as a possible marher for evaluating fetal risk in patients with systemic lupus enythematosus" J.Autoimmunity. 2. 843-849 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Koike,T.: "Immunological aspects of monoclonal anti-cardiolipin antibodies derived from SLE-prone mice" Proc.2nd International Conference on SLE. 146-154 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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