| Project/Area Number |
01570446
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Shinshu University |
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Principal Investigator |
SHOSEI Koh Shinshu University School of Medicine, Assistant Professor, 医学部, 講師 (80143981)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Gangliosides / Experimental autoimmune encephalomyelitis (EAE) / Multiple sclerosis |
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| Research Abstract |
Gangliosides are known to suppress T-cell responses to mitogens and alloantigens. Gangliosides treatment (50mg/kg/rat subcutaneously every 12 hours) suppressed clinical signs of both actively induced and cell-transferred experimental allergic encephalomyelitis (EAE) in Lewis rats and guinea pig myelen basic protein (GPMBP) sensitized and cultured lymphocytes : mean score for gangliosides-treated rats 17.9, controls 32.4 in actively induced EAE ; gangliosides-treated rats 0, controls 1.4 in cell-transferred EAE. Lymph node cells or spleen cells from GPMBP-sensitized rats were stimulated in vitro with the specific sensitizing antigen, GPMBP, on with Con A. Purified bovine brain gangliosides significantly suppressed GPMBP-induced proliferation as a dose dependent manner. Gangliosides treatment during sensitization phase (from day-5 to day 3) did not suppress actively induced EAE. There were no significant differences in T-cell subsets of peripheral blood or spleen cells between gangliosides treated rats and controls by flow cytometry analysis. Taken together, these findings indicate that gangliosides primarily act on the immune effector phase of EAE.
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