|Budget Amount *help
¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1990 : ¥800,000 (Direct Cost : ¥800,000)
Fiscal Year 1989 : ¥1,300,000 (Direct Cost : ¥1,300,000)
In a preceding study, a method of in situ hybridization using biotinlabelled DNA probe was successfully applied to investigate localization of measles virus genome in SSPE brain tissue. However, to facilitate further a spread of this technique, more direct and objective evidence of availability must be shown. For this purpose, polymerase chain reaction-non-radioisotopic detection system was constructed.
Human T-cell leukemia virus type I (HTLV- I) was used as a target of this study, because, it was integrated in nuclei as proviral genome DNA, pathognomonic when detected, and shed on light in relation to AIDS or autoimmune diseases.
First, in mother to child transmission, transplacental transmission of this virus was suggested by a fact that there existed 2 out of 5 cases in which HTLV-I specific bands were amplified from cord blood mononuclear cells.
Second, in central nervous system infection of HTLV-I, that is, HTLV-I associated myelopathy, an active state of infection of this virus was suggested by a fact that remarkably strong signals were amplified from minute amount CSF samples of these patients.
As mentiond above, this system was efficiently applied to clinical research. With more modifications, non-radioisotopic in situ hybridization will share a more important part of clinical investigations.