• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Application of Anti CEA-Immunoliposome to boron neutron capture therapy

Research Project

Project/Area Number 01570741
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionThe Institute of medical Science, University of Tokyo

Principal Investigator

FUJII Yuzo  Institute of Medical Science University of Tokyo Assistant Professor, 医科学研究所, 助手 (40143515)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Hisao  Institute of Atomic Energy, Rikkyo University Associate Professor, 原子力研究所, 助教授 (10062605)
TOMITA Toshio  Institute of Medical Science University of Tokyo Assistant Professor, 医科学研究所, 助手 (00126129)
NARIUCHI Hideo  Institute of Medical Science University of Tokyo Professor, 医科学研究所, 教授 (10012741)
SEKIGUCHI Morimasa  Institute of Medical Science University of Tokyo Professor, 医科学研究所, 教授 (60012712)
高橋 司  東京大学, 医科学研究所, 助手 (90143510)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsBoron neutron capture therapy / ^<10>B compound / Liposome / Anti CEA monoclonal antibody / Thermal neutron / 中性子捕捉療法 / 抗CEAモノクロナ-ル抗体
Research Abstract

We tried to broaden the area of boron neutron capture therapy (BNCT) to the treatment of abdominal cancers.
An immunoliposome containing a ^<10>B-compound has been examined as a selective drug delivery system in boron neutron capture therapy. Liposomes conjugated with monoclonal antibodies specific for carcinoembryonic antigen (CEA) were shown to bind selectively to cells bearing CEA on their surface. The immunoliposomes attached to tumor cells suppressed growth in vitro upon thermal neutron irradiation and suppression was dependent upon the concentration of the ^<10>B-compound in the liposomes and on the density of antibody conjugated to the liposomes. The results suggest that immunoliposomes containing the ^<10>B-compound could act as a selective and efficient carrier of ^<10>B atoms to target tumor cells in boron neutron capture therapy.
The ^<10>B compound solution was injected into a heterograft of human poncreatic carcinoma cells, As PC-1, in a nude mouse. Growth of the heterograft was suppressed after thermal neutron irradiation. Pathologically, necrosis and hyalin degeneration of tumor tissue were recognized only in the irradiated group. Hence, the ^<10>B atoms exerted cytotoxic effect in vivo on As PC-1 tumor cells in dose-dependent manner after thermal neutron irradiation.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 柳衛 宏宣 他: "抗CEAモノクロ-ナル抗体(2Cー8)の作製および抗CEAーimmunoliposomeを用いた中性子捕促療法への応用" Human Cell. 2. 290-296 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 柳衛 宏宣 他: "抗CEAーimmunoliposomeの中性子捕促療法への応用" Biotherapy. 3. 1286-1290 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yanagie H.et al: "Application of boronated anti-CEA immunoliposome to boron neutron captare therapy" J.Cancer Res.Clin.Oncol.116. 477 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yanagie H.et al: "Application of boronated anti-CEA immunoliposome to tumor cell growth inhibition in in vitro boron neutron capture therapy." British J of Cancer. 63(4). 522-526 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 小崎 浩一 他: "ヌ-ドマウス可移植ヒト膵癌への中性子捕促療法の実験的研究" 日本外科学会誌雑. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 藤井 祐三 他: "中性子捕促療法によるヌ-ドマウス移植ヒト膵癌の治療" Oncologia. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yanagie H.et al: "4th international Symposium of Neutron Capture Therapy for Cancer" (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hironobu Yanagie, et al.: "Boron neutron capture therapy using ^<10>B entrapped anti-CEA immunoliposome" Human Cell. 12. 290-296 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hironobu Yanagie, et al.: "Allpication of anti CEA-Immunoliposome to boron neutron capture therapy." Biotherapy. 3. 1286-1290 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hironobu Yanagie, et al.: "Application of anti CEA-Immunoliposome to boron neutron capture therapy." J. Cancer Res. Clin. Oncol.116. 477 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hironobu Yanagie, et al.: "Application of boronated anti CEA immunoliposome to tumor cell growth inhibition in in vitro boron neutron capture therapy." British J of Cancer. 63(4). 522-526 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Koichi Kosaki, et al: "Experimental boron neutron capture therapy with transplantable human pancreatic carcinoma in nude mice." Japanese J of Surgery. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yuzo Fujii, et al.: "Boron neutron capture therapy for nude mice" Oncologia. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hironobu Yanagie^^¨ et al: "Application of bcronated antiーCEA immunoliposome to boron neutron capture therapy model." British Journal of Cancer. 63(4). 522-526 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 柳衛 宏宣他: "抗CEAーImmunoliposomeの中性子捕捉療法への応用" 日本外科学会雑誌. 臨時増刊. 311 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hironobu Yanagie^^¨ et al: "Application of boronated anti CEA immunoliposome to boron neutron capture therapy." Proceeding of 4th International Symposium of Neution Capture Therapy for Cancer.

    • Related Report
      1991 Annual Research Report
  • [Publications] 小崎 浩一,他: "ヌ-ドマウス可移植ヒト膵癌への中性子捕捉療法の実験的研究" 日本外科学会雑誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] 柳衛 宏宣 他: "抗CEAーImmunoliposomeの中性子捕捉療法への応用" 日本外科学会雑誌. 311. 311 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ynagie,H.,et al.: "Application of boronated antiーCEA immunoliposome to boron neutron capture therapy." J.Cancer Res.Clin.Oncol.116. 477 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ynagie,H.,et al.: "Application of boronated antiーCEA immunoliposome to tumour cell growth inhibition in in vitro boron neutron capture therapy model." Brit.J.Cancer. 63. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 柳衛宏宣、他: "抗CEA単クロ-ン抗体(2C-8)の作製および抗CEA-Immunoliposomeを用いた中性子捕捉療法への応用" Human Cell. 2. 290-296 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 柳衛宏宣、他: "抗フェトプロテイン・モノクロ-ナル抗体の中性子捕捉療法への応用" 第9回腫瘍マ-カ-研究会論文集. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 柳衛宏宣、他: "抗CEA-Immunoliposomeの中性子捕捉療法への応用" Biotherapy. 3. 1286-1290 (1989)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi