|Budget Amount *help
¥1,700,000 (Direct Cost : ¥1,700,000)
Fiscal Year 1990 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1989 : ¥1,100,000 (Direct Cost : ¥1,100,000)
In sensitivity tests of chemotherapy alone, hyperthermia alone, or a combination of the two against human urinary tract malignancies making use of the chick embryo method using chorioallantoic membrane, measurement of the weight of the tumor mass was considered to be an effective method of evaluating the antitumor effect. However, in cases in whicha a large part of the tumor is necrotic, this evaluation method did not provide reliable results. At present, using the the MTT method, more accurate quantitative results have been obtained. As a sensitivity test for metastatic tumors using continuously cultured cell lines, DNA is extracted from the metastatic organs of a fertilized chick embryo, specific DNA amplification and immuno-electro-phoresis are performed, and the DNA content is measured. By this method, it was shown that is possible to quantitatively evaluate the anti-tumor effect of various treatment methods.