Studies on the improvement of diagnostic method for prostatitis
Grant-in-Aid for Scientific Research (C).
|Research Institution||Gifu University|
KAWADA Yukimichi Gifu University School of Medicine Urology, Professor, 医学部, 教授 (00021428)
林 秀治 岐阜大学, 医学部, 助手 (80208628)
武田 明久 岐阜大学, 医学部附属病院, 助手 (10171638)
藤広 茂 岐阜大学, 医学部・附属病院, 助手 (90144021)
高橋 義人 岐阜大学, 医学部・附属病院, 助手 (20226905)
ITO Yasuhisa Gifu University School of Medicine Urology, Research Associate, 医学部, 助手 (50223194)
SAITO Akihiro Gifu University School of Medicine Urology, Research Associate, 医学部, 助手 (70170488)
DEGUCHI Takashi Gifu University University Hospital Urology, Assistant Professor, 医学部附属病院, 講師 (40163935)
|Project Fiscal Year
1989 – 1991
Completed(Fiscal Year 1991)
|Budget Amount *help
¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1991 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1990 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1989 : ¥1,000,000 (Direct Cost : ¥1,000,000)
|Keywords||Prostatitis / EPS / Prostatic catheter / Meares & Stamey's method / C. trachomatis / U. urealyticum / PCR / DNA probe / 前立腺炎 / 前立腺カテ-テル / Meares&Stamey法 / C.trachomatis / U.urealyticum / PCR法 / DNA診断法 / Meares & Stamey法 / EPS採取用カテ-テル / 診断法|
To improve the diagnostic method for prostatitis, specific catheter for the collection of prostatic fluid(prostatic catheter)by which prostatic fluid(EPS)can be obtained without contamination by urethral bacteria was newly developed. This prostatic catheter is blind-ended and has 3 side-holes in the distal portion of balloon. To collect EPS using prostatic catheter, catheter is inserted into the urethra, and then balloon is expanded in the bladder. The catheter is slightly extracted during prostatic massage, and EPS is aspirated through the internal lumen of the catheter by syringe. Contamination of EPS collected by use of prostatic catheter by urethral bacteria was studied in 23 male patients who x%, ere proven to have no genitourinary tract infections. Consequently, contamination due to faculative bacteria was avoided in 50% and that due to Ureaplasma urealyticum in 40% of the cases.
Clinical value of prostatic catheter method in the diagnosis of chronic prostatitis was studied by com
paring with Meares & Stamey's method. Both prostatic catheter method and Meares & Stamey's method were performed by cross-over method in 11 patients with high suspicion of chronic prostatitis. Three of 4 cases of bacterial prostatitis by Meares & Stamey's method weibe also diagnosed as bacterial prostatitis by prostaltic catheter method, but remaining one was diagnosed as prostatodynia. One of 3 cases of nonbacterial prostatitis by Meares & Stamey's method was diagnosed as nonbacterial prostatitis by prostatic catheter method, but the other 2 cases were diagnosed as urethritis. Four cases of prostatodynia by bleares & Stamey's method received same diagnosis by prostatic catheter method. In conclusion, 8 of 11 cases received the same diagnosis by two different methods. The remaining 3 cases received different diagnosis. In these 3 cases, however, prostatic catheter method gave more accurate diagnosis.
From the results obtained in this study, we concluded that prostatic catheter method was useful in the diagnosis of chronic prostatitis, especially in differentiating bacterial prostatitis from nonbacterial prostatitis, prostatodynia and urethritis.
C.trachomatisとU.urealyticumの病原的意義の検討は、前立腺カテ-テルを用いても困難であり、前立腺穿刺液について検討する必要のあることが判明した。このため、微量の前立腺穿刺液からも検出可能な感度の高い方法としてpolymerase chain reaction(PCR)法を用いたC.trachomatisとU.urealyticumの検出法を開発し、この検出法を用いて前立腺穿刺液からの両菌の検出を現在継続中である。
Research Output (11results)