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Development of prenatal diagnosis of rubella infection with DNA

Research Project

Project/Area Number 01571274
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionNational Institute of Health

Principal Investigator

KATOW Shigetaka  National Institute of Health, Department of Measles Virus, Chief Researcher, 麻紳ウイルス部, 主任研究官 (20211162)

Co-Investigator(Kenkyū-buntansha) TANABAYASHI Kiyoshi  National Institute of Health, Department of Measles Virus, Researcher, 麻疹ウイルス部, 研究員 (50197505)
SUZUMORI Kaoru  Nagoya City University Medical School, Department of Obstetrics & Gynecology, As, 医学部, 助教授 (80117829)
KAWANA Takashi  Tokyo University Branch Hospital, Department of Obstetrics & Gynecology, Profess, 医学部付属病院・分院・産婦人科, 教授 (90010272)
竹内 薫  国立予防衛生研究所, 麻疹ウイルス部, 研究員 (00192162)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥800,000 (Direct Cost: ¥800,000)
KeywordsRubella Virus / Congenital Rubella Syndrome (CRS) / Polymerase Chain Reaction (PCR) / Pregnancy / Prenatal Diagnosis / Virus Genome / Genome Diagnosis / Chorionic Villi Sampling (CVS) / ウイルスゲノム / DNAプロ-ベ / 胎児診断 / ゲノム診断 / 先天性風疹
Research Abstract

Primary infection with rubella virus during early pregnancy often leads to intrauterine malformations. It is very important to establish the evidence of virus infection in the early gestation by confirmatory laboratory diagnosis. As a result of the ambiguity of serological diagnosis, artificial abortion was adopted in many cases for fear of having babies with congenital malformation. Definite diagnosis of the intrauterine infection, therefore, has been required to avoid unnecessary artificial termination.
We developed simple method for detection of rubelia virus genome in a variety of maternal and fetal tissues. Total tissue RNA extracted by an acid guanidium-thiocyanate-phenol-chloroform method was reverse transcribed in the presence of a set of synthetic primers then amplified through PCR using the same primers. The resulted DNA was subjected to the second PCR using a different set of primers. The PCR products were then electrophoresed in an agarose gel followed by staining with ethidium bromide.
DNA fragments of expected size were amplified from tissues of fetal origin, such as, chorionic villi, amniotic fluids, and umbilical cord blood lymphocytes obtained by biopsy from pregnant women infected with rubella. The PCR products were proven to be specific to rubella virus by direct sequencing of the products.
By the development of reliable diagnostic method of virus infection in fetus based on viral genome detection in addition to serological methods, we could expect reduction in numbers of babies with congenital rubella syndrome and of unnecessary artificial abortion of healthy babies.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 加藤 茂孝他: "マイクロプレ-トでのハイブリダイゼィションによる風疹ウイルスゲノムの検出" 臨床とウイルス. 18. 317-321 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加藤 茂孝他: "Prenatal Diagnasis of Rubella by Genome Detection" New England Journal of Medicine.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 千場 勉他: "風疹診断に関する最新情報" 産婦人科の実際. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加藤 茂孝他: "風紳感染の出生前遺伝子診断" 秋本臨床. 増刊号. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加藤 茂孝: "ウイルス感染症の臨床と病理(分担執筆)" 医学書院, 247 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加藤 茂孝: "ワクチンハンドブック(分担執筆)" 丸善, (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. KATOW, A. YAMADA, K. TAKEUCHI, K. TANABAYASHI & A. SUGIURA: "Detection of rubella virus genome by microplate hybridization. (in Japanese)" Clinical Virology. 18(3). 317-321 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. HOSHIBA, S. KATOW & Y. YABUKI: "Recent information on rubella diagnosis. (in Japanese) Practice in Obstetrics & Gynecology" (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. KATOW & M. FUKUSHIMA: "Prenatal genome diagnosis of rubella infection. (in Japanese)" Japanese Journal of Clinical Medicine. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. KATOW, M. FUKUSHIMA, A. YAMADA, K. TANABAYASHI & T. HOSHIBA: "Prenatal diagnosis of rubella by genome detection." New England Journal of Medicine.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. KATOW: Rubella virus. (in Japanese) in "Pathology and Aspects of Viral Diseases" ed. by Y. AOYAMA, M. MINAMITANI & T. KURATA.Ikaku-Shoin (Tokyo), 133-139 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. KATOW: Rubella Vaccine. (in Japanese) in "Vaccine Handbook" ed. by Association of Research Fellows in National Institute of Health.Maruzen (Tokyo), (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 加藤 茂孝他: "マイクロプレ-トでのハイブリダイゼイションによる風疹ウイルスゲノムの検出" 臨床とウイルス. 18. 317-321 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 千場 勉他: "風疹診断に関する最新情報" 産婦人科の実際. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加藤 茂孝他: "風疹感染の出生前遺伝子診断" 日本臨床. 増刊号. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加藤 茂孝: "ウイルス感染症の臨床と病理(分担執筆)" 医学書院, 247 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加藤 茂孝: "ワクチンハンドブック(分担執筆)" 丸善, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 加藤 茂孝: "マイクロプレ-トでのハイブリダイゼイションによる風疹ウイルスゲノムの検出" 臨床とウイルス. 18. 317-321 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 加藤 茂孝(分担執筆): "ウイルス感染症の臨床と病理" 医学書院, (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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