Mutation and molecular evolution of squash family proteinase inhibitors
Grant-in-Aid for General Scientific Research (C)
|Allocation Type||Single-year Grants|
|Research Institution||Kyoto Institute of Technology|
HARA Saburo Kyoto Institute of Technology Faculty of Engineering and Design Chemistry and Materials Technology Professor, 工芸学部, 教授 (00028193)
|Project Period (FY)
1989 – 1991
Completed(Fiscal Year 1991)
|Budget Amount *help
¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1991 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1990 : ¥700,000 (Direct Cost : ¥700,000)
Fiscal Year 1989 : ¥900,000 (Direct Cost : ¥900,000)
|Keywords||Serine proteinase inhibitor / Squash family inhibitor / Molecular evolution / 分子進化 / 一次構造 / カンピョウ / ニガウリ|
Squash family inhibitors which are included in cucurbitaceae seeds are composed of only 28 amino acid residues. Therefore, we could draw a phylogenetic tree simply by tracing substitutions of amino acids among amino acid sequences of inhibitors.
For this purpose, many kinds of cucurbitaceous seeds were investigated for their proteinase inhibitory activities. From the results, bitter gourd, bottle gourd, gourd and oriental pickling melon were choosed for the purification of inhibitors. By the investigation of the purification method, it was clarified that acetone precipitation and chromatographies on ion-exchangers and reversed phase matrices were the simple and convenient method for the purification of squash family inhibitors.
Total 12 inhibitors were isolated from 4 kinds of cucurbitaceous seeds (4 inhibitors from bitter gourd, 2 from bottle gourd, 3 froin gourd and 3 from oriental pickling inelon) and their amino acid sequences were determined as follows.
Oriental pickling melon
Research Products (13results)