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バイオエナジェティックスの総括的研究

Research Project

Project/Area Number 01617004
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionNagoya University

Principal Investigator

向畑 恭男  名古屋大学, 理学部, 教授 (10028110)

Co-Investigator(Kenkyū-buntansha) 福井 俊郎  大阪大学, 産業科学研究所, 教授 (90029843)
香川 靖雄  自治医科大学, 医学部, 教授 (30048962)
小澤 高将  名古屋大学, 医学部, 教授 (80022771)
安楽 泰宏  東京大学, 理学部, 教授 (20012643)
二井 將光  大阪大学, 産業科学研究所, 教授 (50012646)
Project Period (FY) 1989
Project Status Completed (Fiscal Year 1989)
Budget Amount *help
¥20,100,000 (Direct Cost: ¥20,100,000)
Fiscal Year 1989: ¥20,100,000 (Direct Cost: ¥20,100,000)
Keywordsエネルギー / バイオエナジェティックス / 呼吸酵素系 / 疾病 / エネルギー産生系 / ATPアーゼ / 進化
Research Abstract

生体の行なっているエネルギーの授受と転換は、生命活動の根底をなすものである。バイオエナジェティックスはこの基礎生体反応の過程に関与する多くの事象を研究の対象としている。本研究では、「重点領域研究・バイオエナジェティックス」の全体を総括し、個別および班研究の推進、研究者間の有機的連携、周辺分野との交流、新分野の開拓等を通じて重点領域研究の効果的進展を計ってきた。
本年度に得られた実績は、以下のように要約される。…………1.学会報告、原著論文等を通じ、本重点領域研究に参加している各研究者の研究進捗状況を把握し、各研究のとりまとめ、完成に向けて適切な助言を行なった。2.異なる経験、研究対象、装置等を持つ研究者の間に共同研究を推進した。3.今年度までに得られた特に著しい成果は、論文別刷を配布して各研究者に周知させた。4.総括班に所属する研究者は、新しい親和試薬の開発、エネルギー転換酵素のcDNAの調製、モノクローン抗体の作製、エネルギー転換酵素阻害剤の開発等を積極的に行ない、各研究者の利用に供した。また各研究者の希望によって、クローン化に必要とするオリゴヌクレオチドの作製、蛋白の部分アミノ酸配列の決定等を総括班により行なった。5.総括班は発足時から、エネルギー転換系の疾病の調査研究を行なってきた。本年度は、小沢教授が米・豪の研究者とともにミトコンドリアの遺伝病の研究会を主催し、本研究の運営委員を中心に参加し、最新の成果を発表した。6.平成元年末に、公募研究者を含む全班員の参加による合同班会議を行なった。各研究の進捗状況と成果を評価委員(総括班員より選出した)が厳密な評価と批判を行ない、年度末までの成果を取りまとめるベく指導を行なった。7.来年度に本研究の成果を、英文の成書と和文の報告書により世に問うべく編集方針を検討した。

Report

(1 results)
  • 1989 Annual Research Report
  • Research Products

    (64 results)

All Other

All Publications (64 results)

  • [Publications] Y.Sugiyama: "Isolation of a gene that encodes a new retinal protein,Archaerhodopsin,from Halobacterium,sp.aus-1." J.Biol.Chem.264. 20859-20862 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Maeda: "Structural analysis of gastric(H^++K^+)-ATPase." An.N.Y.Acad.Sci.402. 204-207 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Inatomi: "Amino acid sequence of the α and β subunits of Methanosarcina barkeri ATPase deduced from cloned genes:Similarity to subunits of eukaryotic vacuolar and F_oF_1ーATPases." J.Biol.Chem.264. 10954-10959 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Inatomi: "Dicyclohexylcarbodiimide-binding protein is a subunit of the Methanosarcina barkeri ATPase complex." Biochem.Biophys.Res.Commun.162. 1585-1590 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Eya: "Overproduction of turncated subunit a__ー of H^+-ATPase causes growth inhibition of Escherichia coli." J.Bacteriol.171. 6853-6858 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Hanada: "Uni-site catalysis by Escherichia coli F_1ーATPase with different numbers of bound nucleotides." FEBS Lett.257. 465-467 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Anraku: "Structure and function of the yeast vacuolar membrane proton ATPase" J.Bioenerg.Biomembr.21. 589-603 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] R.Hirata: "Functional molecular masses of vacuolar membrane H^+-ATPase from S__ー.cerevisiae as studied by radiation inactivation analysis." FEBS Lett.244. 397-401 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] R.Hirata: "Molecular structure of a gene,VMA1,encodig the catalytic subunit of H^+-ATPase from vacuolar membranes of S__ー.cerevisiae." J.Biol.Chem.265. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Suzuki: "Structural organization of the human mitochondrial cytochrome c__ー1 gene." J.Biol.Chem.264. 1368-1374 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Ichiki: "Disproportionate dificiency of iron-sulfur clusters and subunits of complex I in mitochondrial encephalomyopathy(MELAS)." Pediatr.Res.25. 194-201 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Nishikimi: "Cloning and sequence analysis of a cDNA encoding the Rieske iron-sulfur protein of rat mitochondrial cytochrome <bc>___ー1 complex." Biochem.Biophys.Res.Commun.159. 19-25 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.W.Linnane: "Mitochondrial DNA mutations as an important contributor to aging and degenerative diseases." Lancet. i. 642-645 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Yoneda: "A maternally inherited mitochondrial DNA mutation in a Japanese family with Leber′s hereditary optic neuropathy." Lancet. i. 1076-1077 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Seki: "Decreased activity of cytochrome c__ー oxidase in the macular mottled mouse:an immuno-electron microscopic study." Acta Neuropathol.17. 465-471 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Suzuki: "Isolation of a single nuclear gene encoding human ubiquinone-binding protein in complex III of mitochondrial respiratory chain." Biochem.Biophys.Res.Commun.161. 371-378 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] W.Sato: "Multiple populations of deleted mitochondrial DNA by a novel gene amplification method." Biochem.Biophys.Res.Commun.162. 664-672 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Hosokowa: "Complementary DNA encoding core protein II of human mitochondrial cytochrome <bc>___ー1 complex:Substantial diversity in deduced primary structure from its yeast counterpart." J.Biol.Chem.264. 13483-13488 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Yoneda: "Pleiotropic molecular defects in energy-transducing complexes in mitochondrial encephalomyopathy(MELAS)." J.Neurol.Sci.92. 143-158 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Tanaka: "Differently deleted mitochondrial genomes in maternally inherited chronic progressive eternal ophthalmoplegia." J.Inher.Metab.Dis.12. 359-362 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Mizuno: "Deficiencies in complex I subunits of the respiratory chain in Parkinson's disease." Biochem.Biophys.Res.Commun.163. 1450-1455 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Tanaka: "Direct sequencing of delected mitochondrial DNA in myopathic patients." Biochem.Biophys.Res.Commun.164. 156-163 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Tanaka-Yamamoto: "Specific amplification of deleted mitochondrial DNA from a myopathic patient and analysis of deleted region with S1 nuclease." Biochim.Biophys.Acta. 1009. 151-155 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Toda: "Cloning and sequencing of a cDNA encoding the precursor to the 24-kDa iron-sulfur protein of human mitochondrial NADH dehydrogenase." Int.J.Biochem.21. 1161-1168 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Nishikimi: "The primary structure of human Rieske iron-sulfur protein of mitochondrial cytochrome<bc>___ー1 complex dedued from cDNA analysis." Biochem.Int.20. 155-160 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Ito: "Mitochondrial cytopathy" Jpn.Circ.J.

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Jinnai: "A case of mitochondrial myopathy,encephalopathy,and lactic acidosis(MELA)due to cytochrome c__ー oxdase deficiency with neurogenic muscular changes." Eur.J.Neurol.

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Seyama: "Mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes with special reference to the mechanism of cerebral manifestations." Acta Neurol.Scan.

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Koga: "Multisystemic cytochrome c__ー oxidase deficiency with clinical characteristics of Leigh′s disease." J.Neurol.Sci.

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Hosokawa: "The primary structure of the precursor to core protein II,a putative member of mitochondrial processing protease family, of rat mitochondrial cytochrome bc1 complex deduced from cDNA sequence analysis." Biochem.Int.

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Tamura: "Novel regulatory enhancer in the nuclear gene of the human mitochondrial ATP synthase β-subunit." J.Biol.Chem.265. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Hara: "Expression of active α-subunit of rat Na^+,K^+-ATPase from the messenger RNA injected into xenopus oocytes." Biochem.Biophys.Res.Commun.163. 102-105 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Satoh: "A monoclonal antibody against horse kidney(Na^+ +K^+)-ATPase inhibits sodium pump and E_2K to E_1 conversion of(Na^+ +K^+)-ATPase from outside of the cell membrane." Biochim.Biophys.Acta. 994. 104-113 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Fujita: "Regulation of the size of light-harvesting antennae in response to light intensity in the green alga Chlorella pyrenoidosa." Plant Cell Physiology. 30. 1029-1037 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Fujita: "Regulation of the stoichiometry of thylakoid components in the model photosynthetic system of cyanophytes:model experiments showing that control of synthesis or supply of Ch1 __ーa can change the stoichiometric relationship between the two photosystems." Plant Cell Physiology. 31. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Uetsuki: "Isolation and characterization of the human chromosomal gene for polypeptide chain elongation factor-1α." J.Biol.Chem.264. 5791-5798 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] E.Muneyuki: "Steady state kinetics of proton translocation catalyzed by thermophilic F_oF_1 ATPase reconstituted in planar bilayer membranes." J.Biol.Chem.264. 6092-6096 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Kagawa: "α_3β_3 complex of thermophilic ATP synthase:Catalysis without the γ-subunit." FEBS Lett.249. 67-69 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Yoshimura: "Hexagonal structure of two-dimensional crystals of the α_3β_3 complex of thermophilic ATP synthase." J.Biochem.106. 958-960 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Endo: "Defective gene in lactic acidosis:Abnormal pyruvate dehydrogenase E1 α-subunit caused by a frame shift." Am.J.Human Genetics. 44. 358-364 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Yuzaki: "Multiple deletions in mitochondrial DNA at direct repeats of nonーD-loop regions in cases of familial mitochondrial myopathy." Biochemical Biophys.Res.Commun.164. 1352-1357 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Ito: "Small-angle neutron scattering studies of the reconstituted TF_1 from thermophilic bacterium with deuterated subunits." J.Molecular Biol.

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Nagata: "Purification,identification,and characterization of two GTP-binding proteins with molecular weights of 25,000 and 21,000 in human platelet cytosol." J.Biol.Chem.264. 17000-17005 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Takagi: "Amino acid sequence studies on cyanogen bromide peptides of chicken caldesmon which bind to calmodulin." J.Biochem.106. 778-783 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Iwabuchi: "Development of extracellular electric pattern around Lapidium roots:Its possible role in root growth and gravitropism." Plotoplasma. 148. 94-100 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Murata: "A model for bacterial flagellar motor:Free energy transduction and self-organization of rotational motion." J.theor Biol.139. 531-559 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] D.H.Hu: "Myosin oligomers as the molecular mass standard in the estimation of molecular mass of nebulin(〜800 kDa)by sodium dodecyl sulfate-polyacrylamide gel electrophoresis." Biomed.Res.10. 165-168 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Matsuno: "Anti-connectin monoclonal antibodies that react with unc-22 gene product bind dense bodies of Caenorhabditis(nematode)bodywall muscle cells." Tissue & Cell. 21. 495-505 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Ogawa: "Regulation in repressor inactivation by recA protein." Advances in Biophysics. 26. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] A.Higashitani: "ATP-independent strand transfer protein from murine spermatocytes,spermatids,and spermatozoa." Exptl.Cell Res.187. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Yamaguchi: "Simulated weightlessness and bone metabolism decrsase of alkaline phpsphatase activity in the femoral diaphysis of rats." Res.Exp.Med.189. 9-14 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Yamaguchi: "Simulated weightlessness and bone metabolism:Decrease of protein and DNA syntheses in the femoral diaphysis of tats." Res.Exp.Med.189. 331-337 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Yamada: "Proton motive force-dependent and -independent protein translocation revealed by an efficient <in>___ー vitro assay system of Escherichia coli." J.Biol.Chem.264. 1723-1728 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Matsuyama: "Energy-dependent <in>___ー vitro translocation of a model protein into Escherichia coli inverted membrane vesicles can take place efficiently in the complete absence of the cytosol fraction." J.Biol.Chem.264. 3583-3587 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Tagaya: "Site-directed mutagenesis of Pro-17 located in the glycine-rich region in adenylate kinase." J.Biol.Chem.264. 990-994 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Tagaya: "Kinetic studies of pyridoxal kinase from pig liver:Slow-binding inhibition by adenosine tetraphosphopyridoxal." Biochemistry. 28. 4670-4675 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Mukohata: "Bioenergetics;molecular biology,biochemistry and pathology" Plenum Press,

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Mukohata: "Molecular Structure,Function and Assembly of the ATP Synthase" Plenum Press,

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Mukohata: "Light in Biology and Midium vol.2" Plenum Press,

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Anraku: "Plant Water Relations and Growth under Stress" Myu K.K.Press, 65-72 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Wada: "Plant Water Relations and Growth under Stress" Myu K.K.Press, 482-484 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Ebashi: "Muscle Energetics" Alan R.Liss,Inc., 239-251 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Ebashi: "Frontiers in Smooth Muscle Research" Alan R.Liss,Inc., 159-165 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Yagi: "Calcium Protein Signaling" Plenum Publishing Corp., 147-154 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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