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In Vitro and In Vivo Function of Platelet-Derived Endothelial Cell Growth Factor

Research Project

Project/Area Number 01870033
Research Category

Grant-in-Aid for Developmental Scientific Research (B).

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionUniversity of Tokyo

Principal Investigator

MIYAGAWA Kiyoshi (1990)  Third Dept. of Int. Med. Medicine Instructor Univ. of Tokyo, 医学部(病)・第三内科, 助手 (40200133)

高久 史麿 (1989)  東京大学, 医学部(病)・第三内科, 教授 (40048955)

Co-Investigator(Kenkyū-buntansha) ISHIKAWA Fuyuki  Third Dept. of Int. Med. Medicine Instructor Univ. of Tokyo, 医学部(病)第三内科, 助手 (30184493)
MIYAZONO Kohei  Third Dept. of Int. Med. Medicine Instructor Univ. of Tokyo, 医学部(病)第三内科, 助手 (90209908)
KITAMURA Kiyoshi  Univ. of Tokyo Lecturer, 医学部(病)中央検査部, 講師 (10186265)
HIRAI Hisamaru  Third Dept. of Int. Med. Lecturer Univ. of Tokyo, 医学部(病)第三内科, 講師 (90181130)
TAKAKU Fumimaro  National Medical Center Director, 病院長 (40048955)
本田 浩章  東京大学, 医学部(病)・第三内科, 医員
萩原 弘一  東京大学, 医学部(病)・第三内科, 医員
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥33,100,000 (Direct Cost: ¥33,100,000)
Fiscal Year 1990: ¥13,500,000 (Direct Cost: ¥13,500,000)
Fiscal Year 1989: ¥19,600,000 (Direct Cost: ¥19,600,000)
KeywordsPlatelet / Endothelial cell / Growth factor / Angiogenesis / Epithelial cell / Placenta / Transcriptional regulation / 血管内皮細胞 / 増殖因子
Research Abstract

Platelet-derived endothelial cell growth factor (PD-ECGF) is a novel type of angiogenic factor which we have succeeded in the cDNA cloning in 1988. In order to elucidate the in vivo roles of this factor, we have investigated the cells producing PD-ECGF, and studied the functions of this factor in vitro and in vivo.
By screening 40 cultured cells, we found that PD-ECGF was produced in some anaplastic thyroid carcinoma cells and a breast cancer cell in large amounts. PD-ECGF lacks a hydrophobic signal sequence, and PD-ECGF was not secreted efficiently from these cells.
PD-ECGF is produced in human placenta as well as in platelets. Immunohistochemical analysis revealed that PD-ECGF is produced by fibroblasts in stromal tissue. Since PD-ECGF acts on both endothelial cells and choriocarcinoma cells, it may play an important role in the development of placenta.
Human PD-ECGF gene was cloned from human placental genomic library, and its promotor region was characterized. It was also shown that PD-ECGF gene is localized in human chromosome 22.
PD-ECGF acts not only on vascular endothelial cells, but on some other cultured cells, such as epithelial cell lines. These results, thus, suggest that PD-ECGF will be clinically important as a wound healing hormone.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] F.Ishikawa, F.Takaku et al.: "Identification of angiogenic activity and the cloning and expression of plateletーderived endothelial cell growth factor." Nature. 338. 557-562 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Usuki, F.Takaku et al,: "Production of plateletーderived endothelial cell growth factor by normal and transformed cells in culture." Proc.Natl.Acad.Sci.USA. 86. 7427-7431 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Miyazono,and F.Takaku: "Plateletーderived growth factors." Blood Reviews. 3. 269-276 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Usuki, K.Miyazono ef al.: "Localization of plateletーderived endothelial cell growth factor in human placenta and purification of an alternatively processed form." Cell Regulation. 1. 577-584 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Miyazono,and F.Takaku: "Structure and function of plateletーderived endothelial cell growth factor." Acta.Haematol.Jpn.(1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Miyazono,and F.Takaku: "Plateletーderived endothelial cell growth factor:Structure and function." Jpn Circul.J.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ishikawa, F., Takaku, F., et al.: "Identification of angiogenic activity and the cloning and expression of platelet-derived endothelial cell growth factor." Nature. 338. 557-562 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Usuki, K., Takaku, F., et al.: "Production of platelet-derived endothelial cell growth factor by normal and transformed cells in culture." Proc. Natl. Acad. Sci. USA. 86. 7427-7431 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyazono, K. and Takaku, F.: "Platelet-derived growth factors, a review." Blood reviews. 3. 269-276 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Usuki, K., Miyazono, K., et al.: "Localization of platelet-derived endothelial cell growth factor in human placenta and purification of an alternatively processed form." Cell Regulation. 1. 577-584 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyazono, K. and Takaku, F.: "Structure and function of platelet-derived endothelial cell growth factor" Acta Haematol. Jpn.(1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyazono, K. and Takaku, F.: "Platelet-derived endothelial cell growth factor ; Structure and function." Jpn. Circul. J.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Usuki,K.Miyazono ef al.: "Localization of plateletーderived endothelial cell growth factor in human placenta and purification of an alternatively processed form." Cell Regulation. 1. 577-584 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Miyazono,and F.Takaku: "Acta.Haematol.Jpn." Structure and function of plateletーderived endothelial cell growth factor. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Miyazono,and F.Takaku: "Plateletーderived endothelial cell growth factor:Structure and function." Jpn.Circul.J.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] F.Ishikawa,K.Miyazono,F.Takaku,et al.: "Identification of angiogenic activity and the cloning and expression of platelet-derived endothelial cell growth factor" Nature. 338. 557-562 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Usuki,F.Ishikawa,K.Miyazono,F.Takaku,et al.: "Production of platelet-derived endothelial cell growth factor by normal and transformed human cells in culture" Proceedings of National Academy of Sciences in USA. 86. 7427-7431 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Miyazono & F.Takaku: "Platelet-deived growth factors(a review)" Blood Reviews. 3. 269-276 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Hagiwara,K.Miyazono,F.Ishikawa,F.Takaku,et al.: "Organization of the human platelet-derived endothelial cell growth factor gene" EMBO Journal. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 宮園浩平,石川冬木,高久史麿: "血管内皮細胞増殖因子" 代謝(臨時増刊号,癌'90). (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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