• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mode of action of sperm-activating peptide

Research Project

Project/Area Number 02044059
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKanazawa University

Principal Investigator

SUZUKI Norio  Kanazawa University, Faculty of Science, 理学部, 教授 (20082133)

Co-Investigator(Kenkyū-buntansha) DAVID L. Gar  テキサス大学, 医学部, 教授
GARBERS david L.  University of Texas Southwestern Medical Center, Department of Pharmacology
GARBERS Davi  テキサス大学, 医学部, 教授
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1992: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsSperm-activating peptide / Guanylate cyclase / Protein phosphatase / Sperm / Sea urchin / Dephosphorylation / Calyculin A / グアニレ-トシクラ-ゼ / プロティンホスファタ-ゼ / リン酸化 / 先体反応
Research Abstract

In the past decade, we purified 74 sperm-activating peptides from the egg jelly of 17 species of sea urchins distributed over five taxonomic orders. These peptides show essentially the same biological effects on sea urchin spermatozoa although the biological effects and structures of the peptides are specific at the ordinal level. With regard to that specificity, these peptides can be classified into five groups. Sperm-activating peptide I (SAP-I : GFDLNGGGVG) crosslinked specifically to a 71 kDa protein and induced an electrophoretic mobility change in the guanylate cyclase of Hemicentrotus pulcherrimus sperm plasma membrane with concomitant dephosphorylation and inactivation of the enzyme. The dephosphorylation and subsequent inactivation of the enzyme were inhibited in the presence of calyculin A. The phosphorylated and dephosphorylated forms of the guanylate cyclase contained about 24 and 4 moles of phosphate per mol protein, respectively. A cDNA clone for the gudnylate cyclase has been isolated from a cDNA library for H. pulcherrimus testes. An open reading frame predicted a protein of 1125 amino acids including an apparent signal peptide of 21 residues ; a single transmembrane domain of 25 amino acids divided the mature protein into an amino-terminal, extracellular domain of 485 amino acids and carboxyl domain of 594 intracellular amino acids. The intracellular domain possessed a kinase-like region and a catalytic region. Nine and fifteen serine residues existed in the kinase-like and the catalytic regions, respectively. We purified three different protein phosphatases from the sperm tails. The activity of these enzyme was inhibited by calyculin A.

Report

(3 results)
  • 1992 Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] H.ABE,H.KINOH,T.OIKAWA & N.SUZUKI: "The egg jelly macromolecule, a fucose sulfate glycoconjugate, originates from the accessory cells of the ovary in the sea urchin Hemicentrotus pulcherrimus" Roux′s Arch Dev.Biol.201. 179-189 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.HARUMi,M.KURITA & N.SUZUKI: "Purification and characterization of sperm creatine kinase and guanylate cyclase of the sea urchin Hemicentrotus pulcherrimus" Develop. Growth and Differ.34. 151-162 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.HARUMI,K.HOSHINO & N.SUZUKI: "Effects of sperm-activating peptide I on Hemicentrotus pulcherrimus spermatozoa in high potassium concentration sea water" Develop.Growth and Differ.34. 163-172 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.YOSHINO & N.SUZUKI: "Two classes of receptors specific for sperm-activating peptide type-III in sand dollar spermatozoa" Eur.J.Biochem.206. 887-893 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.YOSHINO,T.TAKAO,Y.SHIMONISHI & N.SUZUKI: "Sperm-actevating peptide V(SAP-V),a fifth member of sperm-activating peptide family,purified from the egg conditioned media of the heart urchin Brissus agassizii" Comp.Biochem.Physiol. 102B. 691-700 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] N.SUZUKI & K.YOSHINO: "The relationship between amino acid sequences of sperm-activating peptides and the taxonomy of echinoids" Comp.Biochem.Physiol.102B. 679-690 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] H.Abe, H.Kinoh, T.Oikawa & N.Suzuki: "The egg jelly macromolecule, a fucose sulfate glycoconjugate, originates from the accessory cells of the obary in the sea urchin Hemicentrotus pulcherrimus" Roux's Arch. Dev. Biol.201. 179-189 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Harumi, M.Kurita & N.Suzuki: "Purification and characterization of sperm creatine kinase and guanylate cyclase of the sea urchin Hemicentrotus pulcherrimus" Develop. Growth and Differ.34. 151-162 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] T.Harumi, K.Hoshino & N.Suzuki: "Effects of sperm-activating peptide I on Hemicentrotus pulcherrimus spermatozoa in high potassium concentration sea water" Develop. Growth and Differ.34. 163-172 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Yoshino & N.Suzuki: "Two classes of receptors specific for sperm-activating peptide type-III in and dollar spermatozoa" Eur. j. Bochem.206. 887-893 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] K.Yoshino, T.Takao. Y.Shimonishi & N.Suzuki: "Sperm-activating peptide V (SAP-V), a fifth member of sperm-activating peptide family, purified from the egg-conditioned media of the heart urchin Brissus agassizii" Comp. Biochem. Physiol.102B. 691-700 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Yoshino,K.,Takao,T.,Suhara M.,Kitai,T.,Hori,H.,et al.: "Identification of a novel amino acid,o-bromo-L-phenylalanine,in egg-associated peptide which activates spermatozoa." Biochemistry. 30. 6203-6209 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Abe,H.,Kinoh,H.,Oikawa,T.Suzuki,N.: "The egg-jelly macromolecule,a fucose sulfate glycoconjugate,originates from the accessory cells of the ovary in the sea urchin Hemicentrotus pulcherrimus." Rouxs Arch.Dev.Biol.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshino,K.,Takao,T.,Shimonishi,Y.& Suzuki,N.: "Determination of the amino acid sequence of an intramolecular disulfide likage-containing sperm-activating peptide by tandem mass spectrometry." FEBS Lett.294. 179-182 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Harumi,T.,Kurita,M.& Suzuki,N.: "Purification and characterization of sperm creatine kinase and guanylate cyclase of the sea urchin Hemicentrotus pulcherrimus." Develop.Growth & Differ.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Harumi,T.,Hoshino,K.& Suzuki,N.: "Effects of sperm-activating peptide I on Hemicentrotus pulcherrimus spermatozoa in high potassium concentration sea water." Develop.Growth & Differ.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshino,K.& Suzuki,N.: "Two classes of receptors specific for sperm-activating peptide type III in sand dollar spermatozoa." Eur.J.Biochem.

    • Related Report
      1991 Annual Research Report
  • [Publications] Harumi,T.,Yamaguchi,M.& Suzuki,N.: "Biology of Echinodermata" A.A.Balkema,Rotterdam, 590 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] N.Suzuki: "Structure and function of sea urchin eqq jelly molecules" Zool.Sci.7. 355-370 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Mita,N.Ueta,T.Harumi and N.Suzuki: "The influence of an eggーassociated peptide on eneray metabolism in sea urchin spermatozoa: The peptide stimulates preferential hydrolysis of phosphatidylcholine and oxidation of fatty acid" Biochim.Biophys.Acta. 1035. 175-181 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Takao,K.Yoshino,N.Suzuki and Y.Shimonishi: "Analysis of postーtranslational modification of proteins by accurate mass measurement in fast atom bomberdment mass spectrometry" Biomed.Environ.Mass Spectrom.19. 705-712 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Yoshino,T.Takao,M.Yamaguchi,Y.Shimonishi and N.Suzuki: "Derivatives of spermーactivating peptide III (SAPーIII) associated with eggs of sand dollars, Clypeaster japonicus and Astriclypeus manni" Comp.Biochem.Physiol.97B. 679-685 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] R.Harumi,M.Yamaguchi,and N.Suzuki: "Peceptors for spermーactivating peptides,SAPーI and SAPーIIB,on spermatozoa of sea urchins,Hemicentrotus pulcherrimus and Glyptocidaris crenularis" Develop.Growth and Differ.33. 171-176 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Yoshino,T.Takano,H.Hori,M.Suhara,T.Kitai,K.Nomura et al: "Primary structures of bromophenylalanineーcontaining spermーactivating peptides." Peptide Chemistry 1990. 171-176 (1991)

    • Related Report
      1990 Annual Research Report

URL: 

Published: 1990-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi