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Plastid Biogenesis and Its Molecular Biology

Research Project

Project/Area Number 02304005
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionKanazawa University

Principal Investigator

WADA Keishiro  Kanazawa Univ., Fac. Sci., Prof., 理学部, 教授 (70028174)

Co-Investigator(Kenkyū-buntansha) HASE Toshiharu  Nagoya Univ., Fac. Agric., Assoc. Prof., 農学部, 助教授 (00127276)
WATANABE Akira  Nagoya Univ., Fac. Agric., Prof., 農学部, 教授 (70023471)
MURATA Norio  Natl. Instit. Basic Biol., Prof., 教授 (90011569)
TOHYAMA Susumu  Ochanomizu Univ., Fac. Sci., Prof., 理学部, 教授 (20017213)
AKAZAWA Takashi  Nagoya Univ., Fac. Agric., Prof., 農学部, 教授 (20023400)
大山 莞爾  京都大学, 農学部, 教授 (40135546)
佐々木 幸子  京都大学, 農学部, 助手 (00026519)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥10,000,000 (Direct Cost: ¥10,000,000)
Fiscal Year 1991: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1990: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordsplastids / plastid interconversion / chloroplasts / amyloplasts / multigene family / adenylate translocator / DNA methylation / ferredoxins / グリセロ-ル‐3‐リン酸アシルトランスフェラ-ゼ
Research Abstract

A little is known about plastids, such as chromoplasts, amyloplasts, etioplasts and proplastids in the different organs or tissues of plant, with the same DNA molecules as in chloroplasts. The cooperative research project "Plastid Biogenesis and Its Molecular Biology" was organized by eleven excellent researchers in order to-investigate the problems as follows : 1) Expression and regulation of plastid genes ; 2) Plastid biogenesis and interconversion ; 3) Formation and function of enzyme systems in plastids ; 4) Interaction between plastid genes and nuclear genes encoding plastidic proteins ; 5) Information exchanges between the investigators.
Several plastid protein genes on neclear genome are present as the multigene family. It was confirmed with both levels of protein and gene that their expressions are controlled differentially during the plastid formations in the different plant organs. The plastid interconversions regulated by light and plant hormones were intensively observed by electron microscopy, using a new model system of the carrot root cullus. A new double silicone oil centrifugation method was developed and used for evidence that an adenylate translocator on the inner membrane of the isolated amyloplasts specifically incorporates ADPG for starch synthesis and that sucrose synthase is the key enzyme for biosynthesis of ADPG. Three genes of plastid enzymes related to the fatty acid biosynthesis were cloned and one of them was introduced into the tobacco plant. The transgenic tobacco plant showed the differences in unsaturated fatty acid content of chloroplast and chilling tolerancy.
Some other interesting results on DNA methylation in the control regions of gene, the identification of unknown ORFs and the presence of enzyme system for nitrogen metabolism in root plastids were obtained.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] S.Morigasaki,et al.: "Purification and Characterization of a Ferredoxin-NADP^+ Oxidoreductase-like Enzyme from Radish Root Tissues." Plant Physiology. 93. 896-901 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Frehner et al.: "Enzyme Sets of Glycolysis,Gluconeogenesis.and Oxidative Pentose Phosphate Pathway are not Complete in Nongreen Highly Purified Amyloplasts of Sycamore Cell..." Plant Physiology. 94. 538-544 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Ogura et al;: "Gene Encoding a Putative Zinc Finger Protein in Synechocystis PCC 6803." Agric.Biol.Chem.55. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Hase et al.: "Molecular Cloning and Differential Expression of the Maize Ferredoxin Gene Family." Plant Physiology. 96. 77-83 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Sakakibara et al.: "Molecular Cloning and Characterization of Complementary DNA Encoding for Ferredoxin-Dependent Glutamate Synthase in Maize Leaf." J.Biol.Chem.266. 2028-2035 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Kobayashi: "Differentiation of Amyloplasts and Chromoplasts." In Cell Culture and Somatic Cell Genetics of Plants. Vol.7B. 395-415 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Morigasaki, K. Takata, T. Suzuki, K. Wada: "Purification and characterization of a ferredoxin-NADP^+ reductase-like enzyme from radish root tissues" Plant Physiol. 93. 896-901 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Morigasaki, K. Takata, Y. Sanada, K. Wada, B. C. Yee S. Shin, B. B. Buchanan: "Novel forms of ferredoxin and ferredoxin-NADP reductase form photosynthetic and non photosynthetic tissues of tomato." Arch. Biochem. Biophys.283. 75-80 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Frehner, J. Pozueta-Romera, T. Akazawa: "Enzyme sets of glycolysis, gluconeogenesis, and oxidative pentose phosphate pathway are not complete in nongreen highly purified amyloplasts of sycamore cell suspension cultures." Plant Physiol.94. 538-544 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] A. M. Viale, J. Ngernprasirtsiri, T. Akazawa: "Characterization and intraorganellar distribution of protein kinases in amyloplasts isolated from cultured cells of sycamore." Plant Physiol.96. 1142-1149 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Hase, Y. Kimata, K. Yonekura, H. Sakakibara: "Molecular cloning and differential expression of the maize ferredoxin gene family." Plant Physiol.96. 77-83 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Sakakibara, M. Watanabe, T. Hase, T. Sugiyama: "Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthase in maize leaf." Biol. Chem.266. 2028-2035 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Obokata, K. Mikami, N. Hayashida, M. Sugiura: "Polymorphism of a photosystem I subunit caused by alloploidy in Nicotiana." Plant Physiol.92. 273-275 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Yamamoto, H. Tsuji, N. Hayashida, K. Inoue, J. Obokata: "Nucleotide sequence of cDNA clones encoding PSI-D2 protein of photosystem I in Nicotiana sylvestris." Plant Mol. Biol.17. 1251-1254 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Sasaki, K. Sekiguchi, Y. Nagano, R. Matsuno: "Detection of small GTP-binding proteins in the outer envelope membrane of pea chloroplasts." FEBS Lett.293. 124-126 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Nagano, R. Matsuno, Y. Sasaki: "An essential gene of Escherichia coli that has sequence similarity of a chloroplast gene of unknown function." Mol. Gen. Genet.228. 62-64 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Imai, I. Nishida, N. Murata: "Acyl-(acyl-carrier-protein) hydrolase from squash cotyledons specific to long-chain fatty acids : Purification and characterization." Plant Mol. Biol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Kamide, H. Sakai, S. Morigasaki, Y. Sanada, K. Wada: "Specific distribution of ferredoxin isoproteins in photosynthetic and nonphotosynthetic tissues of tomato." Plant Physiol.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Ogura et al.: "Gene Encoding a Putative Zinc Finger Protein in Synechocystis PCC 6803" Agric.Biol.Chem.55. 2259-2264 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] L.S.Green et al.: "Ferredoxin and Ferredoxin‐NADP^+reductase from Photo‐synthetic and NonPhotosynthetic Tissues of Tomato." Plant Physiology. 96. 1207-1213 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Hase et al.: "Molecular Cloning and Differential Expression of the Maize Ferredoxin Gene Family." Plant Physiology. 96. 77-83 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Sakakibara et al.: "Molecular Cloning and Characterization of Complementary DNA Encoding for Ferredoxin‐Dependent Glutamate Synthase in Maize Leaf." J.Biol.Chem.266. 2028-2035 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Morigasaki et al.: "Comparative Studies on Ferredoxin‐NADP^+Oxidoreductase Isoenzymes Derived from Different Organs by Antibodies Specific for Radish Root‐and Leaf‐Enzymes." Plant Physiology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Imai et al.: "Acy1‐(Acy1‐Carrier‐Protein)Hydrolase from Squash Cotyledons Specific to Long‐Chain Fatty Acids:Purification and Characterization." Plant Mol.Biol.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] S.Morigasaki et al.: "Novel forms of ferredoxin and ferredoxinーNADP reductase from spinach roots." Arch.Biochem.Biophys.283. 75-80 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Sasaki et al.: "Correlation of plastid DNA copy number with plastid gene expression in various organs in mature pea plants (<Pisum sativum>___ー L)" Plant Cell Physiol.31. 925-931 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Ngernprasirtsiri and H.Kobayashi: "Application of an efficient strategy with a phage vector for constructing a physical map of the amyloplast geneme of sycamore (<Acer>___ー <pseudoplatanus>___ー)" Arch.Biochem.Biophys.276. 172-179 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Kobayashi et al.: "Transcriptional requlation and DNA methylation in plastids during transitional conversion of chloroplasts to chromoplasts." EMBO J.,. 9. 307-313 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Hase et al.: "Molecular cloning and differential expression of the maize ferredoxin gene family." Plant Physiol.

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Sakakibara et al.: "Molecular cloning and characterization of complementary DNA encoding for ferredoxinーdependent glutamate synthase.in maize leaf." J.Biol.Chem.266. 2028-2035 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] L.S.Green et al.: "Ferredoxin and ferredoxinーNADP reductase from photosynthetic and nonーphotosynthetic tissues of Tomato" Plant Physiol.(1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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