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Structure and Function of Amyloplasts

Research Project

Project/Area Number 02453124
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionNagoya University

Principal Investigator

AKAZAWA Takashi  Nagoya University, School of Agricultural Sciences Professor, 豊学部, 教授 (20023400)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Junji  Nagoya University School of Agricultural Sciences Associate Professor, 農学部, 助手 (10183120)
TAKABE Tetsuko  Nagoya University School of Agricultural Sciences Associate Professor, 農学部, 助教授 (60089852)
林 誠  名古屋大学, 農学部, 日本学術振興会特別研 (50212155)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥4,800,000 (Direct Cost: ¥4,800,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsAmyloplast / Organelle / Starch / Sucrose / Adenylate translocatior / ADPG / Sucrose synthase / Chloroplast / AOPG / 生合成 / トランスロケ-タ- / 遺伝子発現
Research Abstract

1. Establishment of "double silicone-layer centrifuge" method
2. identification of adenylate translocator in plastid membrane
3. Discovery of novel ADP-specific sucrose synthase
4. Proposal of new mechanisms concerning sucrose-starch transion in higher plants

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] J.Ngernprasirtsiri 1989: "DNA methylation and the differential expression of C_4 photosynthesis genes in mesophyll and bundle sheath cells of greening maize leaves" J.Biol.Chem.264. 1841-1848 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Ngernprasirtsiri: "Transcriptional regulation and DNA methylation of nuclear genes for photosynthesis in nongreen plant cells" Proc.Natl.Acad.Sci.USA. 86. 7919-7923 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M.Frehner: "Enzyme sets of glycolysis,gluconeogenesis,and oxidative pentose phosphate pathway are not complete in nongreen highly purified amyloplasts of sycamore(Acer pseudoplatanus L.)cell suspension cultures" Plant Physiol.94. 538-544 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T.Akazawa: "ADPG revisited-nearly 30 years from Buenos Aires and Los Banos to Nagoya" Plant Mol.Biol.Reporter. 9. 145-155 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Pozueta-Romero: "Direct transport of ADP glucose by an adenylate translocator is linked to starch biosynthesis in amyloplasts" Proc.Natl.Acad.Sci.USA. 88. 5769-5773 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Pozueta-Romero: "ADPG formation by the ADP-specific cleavage of sucrose-reassessment sucrose synthase" FEBS Lett.291. 233-237 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Hayashi: "Regulatory role of CA^<2+> on the level of alpha-amylase mRNA in rice seed" Plant Biochem. Physiol.27. 349-354 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri: "DNA methylation and the differential expression of C_4 photosynthesis genes in mesophyll and bundle sheath cells of greening maize leaves"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri: "Transcriptional regulation and DNA methylation of nuclear genes for photosynthesis in nongreen plant cells" Proc. Natl. Acad. Sci. USA. 86. 7919-7923 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri: "Immunological analysis shows that an ATP/ADP-translocator is associated with the inner-envelope membranes of amyloplasts from Acer pseudoplatanus L" Plant Physiol.89. 1024-1027 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Frehner: "Enzyme sets of glycolysis, Gluconeogenesis, and oxidative pentose phosphate pathway are not complete in nongreen highly purified amyloplasts of sycamore (Acer pseudoplatanus L.) cell suspension cultures" Plant Physiol.94. 538-544 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Hayashi: "Structure and biosynthesis of the xylose-containing carbohydrate moiety of rice alpha-amylase" Eur. J. Biochem.191. 287-295 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Kobyayashi: "Transcriptional regulation and DNA methylation in plastids during transitional conversion of chloroplasts to chromoplasts" ENBOJ.9. 307-313 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri: "Modulation of DNA methylation and gene expression in cultured sycamore cells treated by hypomethylating base analog" Eur. J. Biochem.194. 513-520 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri: "DNA methylation is a determinative element of photosynthesis gene expression in amylopasts from liquid-cultured cells of Sycamore (Acer pseudoplatanus L.)" Cell Sturc., Func.15. 285-293 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Ngernprasirtsiri: "Expression of photosynthetic genes is distinctly different between chloroplasts and amyloplasts in the liquid-cultured cells of Sycamore (Acer pseudoplatanus L.)" Cell Struc. Func.15. 273-283 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Akazawa: "ADPG revisited - nearly 30 years from Buenos Aires and Los Banos to Nagoya" Plant Mol. Biol. Reporter. 9. 145-155 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Pozueta-Romero: "Filtering centrifugation through two layers of silicone oil : a method ofr the kinetic analysis of rapid metabolite transport in ogranelles" Cell Struc. Func.16. 357-363 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Pozueta-Romero: "Direct transport of ADP glucose by an adenylate translocatior is linked to starch biosynthesis in amyloplasts" Proc. Natl. Acad. Sci. USA. 88. 5769-5773 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Pozueta-Romero: "Comparative analysis of mitochondrial and amyloplast adenylate translocators" FEBS Lett.287. 62-66 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Pozueta-Romero: "ADPG formation by the ADP-specific cleavage of sucrose-reassessment of sucrose synthase" FEBS Lett.291. 233-237 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J. Yamaguchi: "Isolation and characterization of nuclei form rice embryos" Cell Struc. Func.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] M. Monma: "Ubiquitous distribution of ADP-specific sucrose synthase in plants." Biosci. Biotech. Biochem.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] J.Ngernprasirtsiri 1989: "DNA methylation and the differential expression of C_4 photosynthesis genes in mesophyll and bundle sheath cells of greening maize leaves" J.Biol.Chem.264. 1841-1848 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Ngernprasirtsiri: "Transcriptional regulation and DNA methylation of nuclear genes for photosynthesis in nongreen plant cells" Proc.Natl.Acad.Sci.USA. 86. 7919-7923 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] M.Frehner: "Enzyme sets of glycolysis,gluconeogenesis,and oxidative pentose phosphate pathway are not complete in nongreen highly purified amyloplasts of sycamore(Acer pseudoplatanus L.)cell suspension cultures" Plant Physiol.94. 538-544 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] T.Akazawa: "ADPG revisitedーnearly 30 years from Buenos Aires and Los Banos to Nagoya" Plant Mol.Biol.Reporter. 9. 145-155 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.PozuetaーRomero: "Direct transport of ADPglucose by an adenylate translocator is linked to starch biosynthesis in amyloplasts" Proc.Natl.Acad.Sci.USA. 88. 5769-5773 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.PozuetaーRomero: "ADPG formation by the ADPーspecific cleavage of sucroseーreassessment of sucrose synthase" FEBS Lett.291. 233-237 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] J.Ngernprasirtsiri,H.Kobayashi and T.Akazawa: "DNA methylation is a determinative element of photosynthesis gene expression in amyloplasts from liquidーcultured cells of Sycamore (Acer pseudoplatanus L.)" Cell Structure and Function. 15. 285-293 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] A.M.Viale,H.Kobayashi and T.Akazawa: "Distinct properties of Escherichia coli products of plantーtype ribuloseー1,5ーbisphosphate carboxylase/oxygenase directed by two sets of genes from the photosynthetic bacterium Chromatium vinosum" The Journal of Biological Chemistry. 265. 18386-18392 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Frehner,J.PozuetaーRomero and T.Akazawa: "Enzyme sets of glycolysis,gluconeogenesis,and oxidative pentose phosphate pathway are not complete in nongreen highly purified amyloplasts of Sycamore (Acer psudoplatanus L.) cell suspension cultures" Plant Physiology. 94. 538-544 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.PozuetaーRomero,M.Frehne,A.M.Viale and T.Akazawa: "Direct transport of ADPglucose by adenylate translocator is linked to starch biosynthesis in amyloplasts" PNAS. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Akazawa,J.PozuetaーRomero and F.Ardila: "New aspects of starch biosynthesis in amyloplasts." “Molecular Approaches to Compartmentation and Metabolic Regulation". (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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