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Biochemical Studies on Changes in Fish Muscle Proteins Following Temperature Acclimation

Research Project

Project/Area Number02454081
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionThe University of Tokyo

Principal Investigator

WATABE Shugo  Associate Professor, Fac. Agric., Univ. Tokyo, 農学部, 助教授 (40111489)

Project Period (FY) 1990 – 1991
Project Status Completed(Fiscal Year 1991)
Budget Amount *help
¥6,700,000 (Direct Cost : ¥6,700,000)
Fiscal Year 1991 : ¥2,100,000 (Direct Cost : ¥2,100,000)
Fiscal Year 1990 : ¥4,600,000 (Direct Cost : ¥4,600,000)
KeywordsCarp / Goldfish / Temperature acclimation / Myosin / Myofibril / Myosin subfragment-l / ATPase ativity / thermal stability / Lーメロミオシン / 筋肉 / アクチン
Research Abstract

Since fish myofibrillar proteins account for over 50% in total muscle proteins, the feasibility of various kinds of fish meat for food processing and their stability during storage are regarded to be greatly influenced by the properties of myofibrillar proteins. This study was performed to answer the questions on which components and parts are most critical in changes of myofibrillar proteins of carp and goldfish during temperature acclimation, thus providing fisheries industry with valuable and foundamental information on fish muscles as food materials.
1. Several individuals of carp were acclimated to either 10 or 30゚C for a minimum of 1 month and subjected to the preparation of myofibrils. Myofibrillar Mg^<2+>-ATPase activity of 10゚C-acclimated carp was significantly higher than that of 30゚C-acclimated fish when assayed at the same reaction temperatures. In contrast, the thermal stability at 40゚C of myofibrillar Ca^<2+->-ATPase of 10゚C-acclimated carp was several times lower than tha … More t of 30゚C-acclimated one. The similar results were obtained when acclimated goldfish were subjected to the same experiments.
2. Myosin and its subfragment-1 (Sl) from 10゚C-acclimated carp showed a higher maximal initial velocity (Vmax) in actin-activated Mg^<2+>-ATPase activity than the 30゚C-acclimated counterparts, respectively, in a good agreement with the differences observed in myfibrillar Mg^<2+>-ATPase activity between the two acclimated groups. The 30゚C-acclimated myosin and Sl were again more thermostable than the 10゚C-acclimated counterparts, respectively.
3. When a filamentous part of the myosin molecule, rod, was subjected to a limited proteolysis using alpha-chymotrypsin, the 10゚C-acclimated carp exhibited one L-meromyosin band in SDS-PAGE analysis. On the other hand, the 30゚C-acclimated carp gave three L-meromyosin bands. one of which was identical to the 10゚C-acclimated L-meromyosin.
4. Peptide maps of myosin and Sl heavy chains and rod performed in SDS-gels were clearly different between 10゚C-and 30゚C-acclimated carp, suggesting that the primary structure of carp myosin molecule is altered during temperature acclimation. Less

Report

(3results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report

Research Products

(15results)

All Other

All Publications

  • [Publications] Watabe,Shugo: "Changes in rigor-mortis progress of carp induced by temperature acclimation" Agricultural and Biological Chemistry. 54. 219-221 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hwang,Gyu-Chul: "Changes in carp myosin ATPase induced by temperature acclimation" Journal of Comparative Physiology B. 160. 233-239 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hwang,Gyu-Chul: "The effect of thermal acclimation on rigor mortis progress of carp stored at different temperatures" Nippon Suisan Gakkaishi. 57. 541-548 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hwang,Gyu-Chul: "Changes of carp myosin subfragment-1 induced by temperature acclimation" Journal of Comparative Physiology B. 161. 141-146 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Watabe,Shugo: "Fast skeletal myosin isoforms in thermally acclimated carp" The Journal of Biochemistry. 111. 113-122 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shugo, Watabe: "Changes in rigor-mortis progress in carp induced by temperature acclimation." Agric. Biol. Chem.54. 219-221 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Gyu-Chul, Hwang: "Changes in carp myosin ATPase induced by temperature acclimation" J. Comp. Physiol. B. 160. 233-249 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Gyu-Chul, Hwang: "The effect of thermal acclimation on rigor mortis progress of carp stored at different temperatures" Nippon Suisan Gakkaishi. 57. 541-548 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Gyu-Chul, Hwang: "Changes in carp myosin subfragment-1 induced by temperature acclimation." J. Comp. Physiol. B. 161. 141-146 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shugo, Watabe: "Fast skeletal myosin isoforms in thermally acclimated carp." J. Biochem.111. 113-122 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hwang,GyuーChul: "The effect of thermal acclimation on rigor mortis progress of carp stored at different temperatures" Nippon Suisan Gakkaishi. 57. 541-548 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hwang,GyuーChul: "Changes of carp myosin subfragmentー1 induced by temperature acclimation" Journal of Compartive Physiolgy B. 161. 141-146 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Watabe,Shugo: "Fast skeletal myosin isoforms in thermally acclimated carp" The Journal of Biochemistry. 111. 113-122 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 渡部 終五: "Changes in rigorーmortis progress of carp induced by temperature acclimation" Agricultural and Biological Chemistry. 54. 219-221 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 黄 奎哲: "Changes in carp myosin ATPase induced by temperature acclimation" Journal of Comparative Physiology B. 160. 233-239 (1990)

    • Related Report
      1990 Annual Research Report

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Published : 1990-04-01   Modified : 2016-04-21  

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