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Physiological roles of tachykinin peptides and their receptor subtypes

Research Project

Project/Area Number 02454140
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionFukuoka University

Principal Investigator

KAMIYA Hiro-o  Fukuoka Univ., Dept. of Pharmacol., Prof., 薬学部, 教授 (90028366)

Co-Investigator(Kenkyū-buntansha) HONDA Kenji  Fukuoka Univ., Dept. of Pharmacol., Assistant Prof., 薬学部, 助手 (60140761)
SAITO Ryo  Fukuoka Univ., Dept. of Pharmacol., Assistant Prof., 薬学部, 助手 (80122696)
TAKANO Yukio  Fukuoka Univ., Dept. of Pharmacol., Assistant Prof., 薬学部, 助手 (50113246)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1992: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1991: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1990: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordssubstance P / tachykinins / cardiovascular regulation / vasopressin / NK-1 receptor / blood pressure / hypothalamus / タキキニン受容体 / NKー1受容体 / NKー3受容体 / NKー1受容体刺激薬,CPー96,345 / 細胞内情報伝達系 / 血管弛緩 / 血管内皮 / カルシウム / 情報伝達
Research Abstract

1. Central cardiovascular regulation of tachykinin peptides.
The central pressor responses due to substance P (SP), neurokinin A (NKA) and neuropeptidegamma (NPgamma) were blocked by sympathetic blocking agents. These results suggest that central SP, NKA and NPgamma, derived from the preprotachykinin A gene, increase the blood pressure (BP) and heart rate via sympathetic nerve activity. In contrast, the pressor response to an neurokinin B (NKB) analogue senktide was inhibited by a vasopressin antagonist, and senktide caused an increase in plasma vasopressin level. In addition, injection of senktide into the paraventricularnucleus (PVN) of the hypothalamus also increased the BP. These results suggest that central NKB, derived from the preprotachykinin B gene, stimulated the NK-3 receptor in the PVN, and increase the BP via release of vasopressin from the pituitary gland.
2. Tachykinin receptor subtype in the endothelial cell and vasodilation.
Agonists for the NK-1 tachykinin receptor elicited the potent, transient and endothelium-dependent relaxation. SP-induced relaxation and increase of cGMP content were inhibited by hemoglobin, methylene blue,L-NG-monomethyl-D-arginine. These results suggest that the relaxation induced by SP is mediated by endothelium-derived relaxingfactor (EDRF). In addition, we examined ^<125>I-SP binding to the endothelial cell membranes of porcine aorta.The cells had a single high affinity binding site with Kd=0.10 nM and Bmax=52.2 fmol/mg protein.

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (74 results)

All Other

All Publications (74 results)

  • [Publications] Takano,Y.: "Role of central tachykinin peptides in cardiovascular regulation in rats." Brain Res.528. 231-237 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saito,R.: "Characterization of tachykinin receptors in endothelial cells of porcine atrery." Neurosci.Lett.110. 337-342 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsumoto,H.: "Structure-activity relationships of dimeric analogs of tachykinin peptides:Correlation between spacer length and biological activities." Peptide Chemistry. 1989. 185-188 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 神谷 大雄: "タキキニン受容体の細分化" 医学のあゆみ. 152. 91- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hagio,T.: "The central pressor actions of a novel tachykinin peptide,γ-preprotachykinin-(72-92)-peptide amide(NPg):Involvement of the sympathetic nervous system." Eur.J.Pharmacol.192. 173-176 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Murozono,T.: "Synthesis and biological activity of neurokinin A analogs containing S-activated cysteine residue at N-terminal portion." Peptide Chemistry. 1990. 265-268 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano,Y.: "Role of NK-1 receptor in central cardiovascular regulation in rats:Studies on a novel non-peptide antagonist,CP-96,345 of substance P NK-1 receptor." Neurosci.Lett.139. 122-125 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakayama,Y.: "Central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the rat hypothalamus." Brain Res.595. 339-342 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Murozono,T.: "Neurokinin A analogs containing S-activated cysteine for affinity labelling of neurokinin-2 receptor." Peptide Chemistry. 1991. 275-278 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsumoto,H.: "Optimum cross-linking spacer length of dimeric neurokinin B analogs for interaction with NK-1 tachykinin receptors." Bull.Chem.Soc.Jpn.66. 1-9 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano,Y.: "The mechnism of central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the hypothalamus in rats." Reg.Peptide.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saigo,H.: "Role of NK-1 receptor in central cardiovascular regulation in rast:studies on a novel non-peptide antagonist,CP-96,345,of substance P NK-1 receptor." Reg.Peptide.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matumoto,H.: "Contractile activity of Trimeresurus flavoviridis phospholipase A_2 on guinea pig ileum and artery." Biochemistry Intermational. 24. 181-186 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Honda,K.: "Relationship between substance P-induced initial transient contractions and inositol phospholipid hydrolysis in guinea pig ileum." J.Pharmacobio-Dyn.14. 393-397 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano,Y.: "Tachykinin receptor subtypes:Cardiovasculas roles of tachykinin peptides." Asia Pacific Journal Pharmacol.6. 341-348 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 高野 行夫: "脳タキキニン・ペプチド受容体サブタイプと循環調節:新しいタキキニン・ペプチドneuropeptide γについて" 神経化学. 29. 400-401 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 高野 行夫: "タキキニン・ペプチド受容体と心血管作用機序" 最新医学. 46. 142-149 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 斎藤 亮: "タキキニン・ペプチドによる内皮細胞依存性血管弛緩作用" 福岡大学薬学紀要. 16. 1-12 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kamiya,H.: "Neuroreceptor Mechanisms in Brain(Eds.Kito,S.et al.)Plenim Press,New York" Evidence for cardiovascular roles of tachykinin peptides in the brain of the rat., 27-37 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano,Y.: "Ann.N.Y.Acad.Sci.632" Tachykinin receptor sibtype:Central cardiovascular regulation of tachykinin peptides., 476-478 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saito,R.: "Ann.N.Y.Acad.Sci.632" Pharmacological properties of the tachykinin receptor sibtype in the endotherial cell and vasodilation., 457-459 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano, Y.: "Role of central tachykinin peptides in cardiovascular regulation in rats." Brain Res.528. 231-237 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saito, R.: "Characterization of tachykinin receptors in endothelial cells of porcine artery." Neurosci. Lett.110. 337-342 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsumoto, H.: "Structure-activity relationships of dimeric analogs of tachykinin peptides: Correlation between spacer length and biological activities." Peptide Chemistry. 1989. 185-188 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kamiya, H. and Takano, Y.: "Tachykinin receptor subtypes (in Japanese)" Igakuno-Ayumi. 152. 91 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Hagio, T.: "The central pressor actions of a novel tachykinin peptide, gamma-preprotachykinin-(72-92)-peptide amide (NPgamma): Involvement of the sympathetic nervous system." European J. Pharmacol.192. 173-176 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Murozono, T.: "Synthesis and biological activity of neurokinin A analogs containing S-activated cysteine residue at N-terminal portion." Peptide Chemistry. 1990. 265-268 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsumoto, H.: "Contractile activity of Trimeresurus flavoviridis phospholipase A2 on guinea pig ileum and artery." Biochemistry International. 24. 181-186 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Honda, K.: "Relationship between substance P-induced initial transient contractions and inositol phospholipid hydrolysis in guinea pig ileum." J. Pharmacobio-Dyn.14. 393-397 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano, Y. and Kamiya, H.: "Tachykinin receptor subtypes: Cardiovascular roles of tachykinin peptides." Asia Pacific J. Pharmacol.6. 341-348 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano, Y. and Kamiya, H.: "Tachykinin Receptor Subtypes; Cardiovascular roles of tachykinin peptides. (in Japanese)" The Saishin-Igaku. 46. 142-149 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saito, R.: "Endothelium-dependent vasodilation induced by tachykinin peptide. (in Japanese)" Pharmaceutical Bulletin of Fukuoka Univ. 16. 1-12 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano, Y.: "Role of NK-1 receptor in central cardiovascular regulation in rats: Studies on a novel non-peptide antagonist, CP-96,345 of substance P NK-1 receptor." Neurosci. Lett.139. 122-125 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nakayama, Y.: "Central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the rathypothalamus." Brain Res.595. 339-342 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Murozono, T.: "Neurokinin A analogs containing S-activated cysteine for affinity labelling of neurokinin-2 receptor." Peptide Chemistry. 1991. 275-278 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Matsumoto, H.: "Optimum cross-linking spacer length of dimeric neurokininB analogs for interaction with NK-1 tachykinin receptors." Bull. Chem. Soc. Jpn.66. 1-9 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Kamiya, H.: "Evidence for cardiovascular roles of tachykinin peptides in the brain of the rat." Neuroreceptor Mechanisms in Brain (Eds. Kito, S. et al.)Plenum Press, New York. 27-37 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano, Y.: "Tachykinin receptor subtype: Central cardiovascular regulation of tachykinin peptides." Ann. N.Y. Acad. Sci.632. 476-478 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saito, R.: "Pharmacological properties of the tachykinin receptor subtype in the endotherial cell and vasodilation." Ann. N.Y. Acad. Sci.632. 457-459 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano, Y.: "The mechanism of central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the hypothalamus in rats." Reg. Peptide. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Saigo, A.: "Role of NK-1 receptor in central cardiovascular regulation in rats : studies on a novel non-peptide antagonist, CP-96,345, of substance P NK-1 receptor." Reg. Peptide. (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Takano,Y.: "Role of NK-1 receptor in central cardiovascular regulation in rats:Studies on a novel non-peptide antagonist,CP-96,345 of substance P NK-1 receptor." Neurosci.Letts.139. 122-125 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Nakayama,Y.: "Central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the rat hypothalamus." Brain Res.595. 339-342 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Murozono,T.: "Neurokinin A analogs containing S-activated cysteine for affinity labelling of neurokinin-2 receptor." Peptide Chemistry. 1991. 275-278 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] Matsumoto,H.: "Optimum cross-linking spacer length of dimeric neurokinin B analogs for interaction with NK-1 tachykinin receptors." Bull.Chem.Soc.Jpn.66. 1-9 (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takano,Y.: "The mechanism of central pressor actions of tachykinin NK-3 receptor in the paraventricular nucleus of the hypothalamus in rats." Reg.Peptide.

    • Related Report
      1992 Annual Research Report
  • [Publications] Saigo,H.: "Role of NK-1 receptor in central cardiovascular regulation in rats:studies on a novel non-peptide antagonist,CP-96,345,of substance P NK-1 receptor." Reg.Peptide.

    • Related Report
      1992 Annual Research Report
  • [Publications] Matumoto,H.: "Contractile activity of Trimeresurus flavoviridis phospholipase A_2 on guinea pig ileum and artery." Biochemistry International. 24. 181 186 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Honda,K.: "Relationship between substance P-induced initial transient contractions and inositol Phosphlipid hydrolysis in guinea pig ileum." J.Pharmacobio-Dyn.14. 393 397 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Takano,Y.: "Tachykinin receptor subtypes:Cardiovasculas roles of tachykinin peptides." Asia Pacific Journal Pharmacol.6. 341 348 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 高野 行夫: "脳タキキニン・ペプチド受容体サブタイプと循環調節:新しいタキキニン・ペプチドneuropeptide γ について" 神経化学. 29. 400 401 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 高野 行夫: "タキキニン・ペプチド受容体と心血管作用機序" 最新医学. 46. 142 149 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] 斎藤 亮: "タキキニン・ペプチドによる内皮細胞依存性血管弛緩作用" 福岡大学薬学紀要. 16. 1 12 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Saito,R.: "Ann.N.Y.Acad.Sci.632" Pharmacological properties of the tachykinin receptor subtype in the endotherial cell and vasodilation, 457 459 (1991)

    • Related Report
      1992 Annual Research Report
  • [Publications] Murozono,T.: "Synthesis and biological activity of neurokinin A analogs containing S-activated cysteine residne at N-terminal portion." Peptide Chemistry. 1990. 265-268 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Matsumoto,H.: "Contractile activity of Trimeresurus flavoviridis phospholipase A2 on guinea pig ileum and artery." Biochemistry International. 24. 181-186 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Honda,K.: "Relationship between substance P-induced initial transient contractions and inositol phospholipid hydrolysis in guinea pig ileum." J.Pharmacobio-Dyn.14. 393-397 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takano,Y.: "Tachykinin receptor subtypes:Cardiovascular roles of tachykinin peptides." Asia Pacific J.Pharmacol.6. 341-348 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高野 行夫: "脳タキキニン・ペプチド受容体サブタイプと循環調節:新しいタキキニン・ペプチドneuropeptideγについて" 神経化学. 29. 400-401 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 高野 行夫: "タキキニン・ペプチド受容体と心血管作用機序" 最新医学. 46. 142-149 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takano,Y.: "Role of NK-1 receptor in central cardiovascular regulation in rats:Studies on a novel non-peptide antagonist,CP-96,345 of substance PNK-1 receptor." Nearosci.Lett.1992

    • Related Report
      1991 Annual Research Report
  • [Publications] Takano,Y.: "Enhancement of striatal dopamine by nicotinic drags “Presynaptic Regulation of Neuro transmitter Release":A handbook." Freund Publishing House (Israel), 593-612 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kamiya,H.: "Evidence for cardiovascular roles of tachykinin peptides inthe brain of the rat,“Neuroreceptor Mechanisms in Brain"" Plenum Press (New York), 27-37 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takano,Y.(ed.by S.E.Leeman et al.): "Tachykinin receptor subtype:Central cardiovascular regulation of tachykinin peptides."substance P and related peptides"" Annals.New Acad.Sci.,New York, 476-P478 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Saito,R.(ed,by,S,E,Leeman et al): "Pharmacological properties of the tachykinin receptor subtype in the endotherial cell and vasodilation.“Substance Pand related peptides"" Annals.New Acad.Sci.,New York, 457-P459 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takano,Y.: "Role of central tachykinin peptides in cardiovascular regulation in rats." Brain Research,. 528. 231-237 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Saito,R.: "Characterization of tachykinin receptors in endothelial cells of porcine artery." Neuroscience Lett.,. 110. 337-342 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsumoto,H.: "Structureーactivity relationships of dimeric analogs of tachykinin peptides:Correlation between spacer length and biological activities." Peptide Chemistry,. 1989. 185-188 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 神谷 大雄: "タキキニン受容体の細分化" 医学のあゆみ. 152. 152-152 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高野 行夫: "タキキニン・ペプチド受容体と心血管作用機序" 最新医学,. 46. 142-149 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hagio,T.: "The central pressor actions of a novel tachykinin peptide,γーpreprotachykininー(72ー92)ーpeptide amide(NPγ):Involvement of the sympathetic nervous system." European J.Pharmacol.,. 192. 173-176 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takano,Y.: "Tachykinin receptor subtype:Central cardiovascular regulation of tachykinin peptides." Annals.New Acad.Sci.,.

    • Related Report
      1990 Annual Research Report
  • [Publications] Saito,R.: "Pharmacological properties of the tachykinin receptor subtype in the endotherial cell and vasodilation." Annals.New Acad.Sci.,.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kamiya,H.: "Neurotransmitter Receptors(ed.by S.Kito et al.)" Plenum Publishing,

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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