| Project/Area Number |
02557097
|
|---|
| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
医学一般
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
TANIGUCHI Naoyuki Osaka Univ. Med. Sch. Professor, 医学部, 教授 (90002188)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UDA Taizou Ube Res. Lab. Ube. Ind. Ltd. Researcher, 主任研究員
TADA Michihiko Osaka Univ. Med. Sch. Professor, 医学部, 教授 (90093434)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥10,400,000 (Direct Cost: ¥10,400,000)
Fiscal Year 1991: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1990: ¥8,100,000 (Direct Cost: ¥8,100,000)
|
|---|
| Keywords | Superoxide Dismutase / Enzyme Immumoassay / Myocardial Injury / Acute Myocardial Infarction / Cardiac Function / Tumor Necrosis Factor / ス-パ-オキサイドディマムタ-ゼ / MnーSuperoxide Dismutase / Myocardial infaraction / Tumor Necrosis Factor / Serum / Myocytes |
|---|
| Research Abstract |
An enzyme-linked immunosorbent assay (ELISA) has been developed for human manganese-superoxide dismutase (Mn-SOD), using a specific monoclonal antibody raised against the purified enzyme. The MN-SOD molecule comprises four identical sub-units this permitted the development of a symmetrical assay, using the same monoclonal antibody as both capture and detector. The assay offers a specific, sensitive and convenient means of measuring immunoreactive MN-SOD in human sera. Under optimum conditions, the sensitivily of the assay permits the detection of 2-200 ng of purified MN-SOD from human liver. The mean serum Mn-SOD levels of normal healthy males and females were 99.8 <plus-minus> 24.8. (mean <plus-minus> SD) and 88.8 <plus-minus> 20.8 (mean <plus-minus> SD), respectively. Serum MN-SOD levels were determined in patients with acute myocardial infarction by ELISA. The serial determinations performed on 29 patients with acute myocardial infarction indicated that MN-SOD appears in serum in a biphasic manner ; an early small elevation was observed at approximately 16.2 (<plus-minus> 7.3) h and a high elevation at apporoximately 108 (<plus-minus> 20.6) h after the onset of symptoms. A negative correlation was found between the maximum value of the late elevation of MN-SOD and left ventricul
|
