|Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1990: ¥1,700,000 (Direct Cost: ¥1,700,000)
The changing patters for total soluble proteins in the bark and leaf tissues differed among apple, Japanese pear, mume, grape and citrus. In apple and citrus, total soluble proteins were accumulated in the bark and leaf tissues during summer season. However apple leaves lost some proteins during senescence, and the increase of total soluble proteins in the bark continued. The protein contents in the bark and leaf tissues of citrus increased until late January. In next spring, the strage proteins were reutilized for developing flower buds and leaves of apple and citrus. Total protein contends in the bark and leaf tissues of grape and in leaf tissue of Japanese pear increased during shoot growing, but their decline began in early August. In contrast, total protein contends in the bark tissue of Japanese pear and in the bark and leaf tissues of mume scarcely changed throughout the year.
When various plant growth regulators were applied to the cut shoots of apple and Japanese pear, total protein contents in the bark decreased with applications of ABA, GA_<4+7> and NAA in apple and of ABA andKT-30 in Japanese pear.
The gibberellins identified in extracts from Japanese pear shoot by full-scan combined gas chromatography-mass spectrometry(GC-MS) were GA_9. GA_<19>. GA_<23>. GA_<24>. GA_<44>, GA_<53> and 3-epi-GA_4. GA_<19> and GA_<23> were major gibberellins in Japanese pear shoot. The levels of both gibberellins decreased from June to August. However, the levels of two gibberellins in the shoots of cultivar which flower-bud formation was inferior increased at the time of flower-bud differentiation.