|Budget Amount *help
¥2,000,000 (Direct Cost : ¥2,000,000)
Fiscal Year 1991 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1990 : ¥1,400,000 (Direct Cost : ¥1,400,000)
(1)Three kinds of alpha-glucosidases(HbG-I, HbG-II and HbG-III)were purified from honeybee. In addition, an alpha-glucosidase was also purified from honey.
(2)HbG-I, II and III were immunologically confirmed to be distributed in ventriculus, hemolymph(and also ventriculus)and hypopharyngeal gland, respectively. The alpha-glucosidase from honey was identified to be the same enzyme as HbG-III.
(3)HbG-I and II showed allosteric behaviors. These two enzymes were regarded as so-called hysteretic enzyme which showed a relatively slow conformational change in second or minute order.
(4)The subsites affinities in active site of HbG-I, II and III were evaluated. Subsite 1, 2 and 3 having positive values in three enzymes were considered to be effective for binding of substrate to active site.
(5)Honeybee alpha-glucosidase, which is the same enzyme as HbG-III, was considered to be secreted into honeydew from hypopharyngeal gland.