| Project/Area Number |
02660088
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Mie University |
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Principal Investigator |
TAKAHASHI Takao Mie University,Faculty of Bioresources, Professor, 生物資源学部, 教授 (10024556)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1992: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Lectins / Lymphocyte transformation / Blast cells / DNA polymerase / Chondroitin sulfate / DNAポリメラ-ゼ / リンパ球幼若化 / リンパ球の生存率 / コンドロイチン硫酸 / リンパ球の表面荷電 |
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| Research Abstract |
It is known that the surface charge of lymphocytes is of importance for their mitogen-induced transformation. This was suggested from the fact that the treatment of rat lymph-node lymphocytes with neuraminidase or the addition of polycations, poly -L-ornithine or poly-D-lysine, enhanced their response to mitogens. Other experiments by previous workers showed that some glycoproteins found in normal serum interfered with the in vitro response of lymphocytes to mitogens. One characteristic of these glycoproteins such as alpha-glycoproteins is a relatively high sialic acid content. Larsen and Heron reported that the mitogen-induced response of human lymphocytes was enhanced by such polycationic compounds as poly-D-lysine, DEAE-dextran, protamin sulfate and methylated serum albumin, whereas it was inhibited by the polyanionic compounds, ceruloplasmin and chondroitin sulfate. I report the effects of chondroitin sulfate A on the lymphocyte mitogenesis induced by concanavalin A. A low concentration of chondroitin sulfate enhanced a suboptimal dose of concanavalin A -induced response of lymphocytes. Chondroitin sulfate increased the incorporation of ^3H-thymidine and the number of blast cells in lymphocyte mitogenesis induced by a suboptimal dose of concanavalin A. Chondroitin sulfate increased the viability and the cell surface charge of lymphocytes in the absence of concanavalin A, but it did not affect the concanavalin A -induced Ca^<2+> influx into lymphocytes.
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