|Budget Amount *help
¥2,200,000 (Direct Cost : ¥2,200,000)
Fiscal Year 1991 : ¥600,000 (Direct Cost : ¥600,000)
Fiscal Year 1990 : ¥1,600,000 (Direct Cost : ¥1,600,000)
1)Acremoauxin A(AA-A), a new potent auxin derivative produced by Acremonium roseum I4267, was synthesized from 2, 3 : 4, 5-di-0-isopropylidene-D-arabitol prepared from Dmannitol and(+)-2-(3-indolyl)propionic acid[(+)-IPA, obtained from(<plus-minus>)-IPA by biological resolution using A. roseum I42671. The synthetically confirmed structure of AA-A was 1-0-[(2S)-2-(3-indolyl)propionyll-D-arabitol. Its related compound, AA-B chromatographically detected from this fungus, was similarly synthesized from the diisopropylidene-D-arabitol and(3-indolyl)acetic acid(IAA), and synthetic AA-B was in fair agreement with natural one in TLC, HPLC and ^1H-NMR.
2)Effects of the var4-ous auxins including AA-A with or without BA on the growth of the calli from Sugi and Akamatsu were investigated on the Murasige & Skoog's media. Results indicated the following orders in activities of callus initiation and propagation. Explant : Sugi triploid>its diploid>Akamatsu, auxins : NAA, 2, 4-D>IBA, 5, 6-DIAA>IPA, AA-A, IAA.
3)Effects of AA-A and the enantiomers of IPA on the growth of plant seedlings were examined. AA-A markedly inhibited the growth of the seedlings of Chinese cabbage, lettuce and oat, but its inhibitory activity against these seedlings was weaker than that of IPA. In tests with 5 species of plant seedlings, (+)-IPA showed the growth inhibition higher than that of its enantiomer. Two seedlings of rice and radish, however, were highly inhibited in the growth by(-)-IPA rather than(+)-IPA.