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Investigation on the relationship between digesta retention time and microbial digestion in the large intestine

Research Project

Project/Area Number 02660278
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionOkayama University

Principal Investigator

SAKAGUCHI Ei  Okayama University, Fac. of Agriculture, Associate professor, 農学部, 助教授 (40170584)

Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1992: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1991: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsLarge intestine / Digesta retention / Fiber digestion / Rodents / Microbes / Short chain fatty acids / Rabbits / 繊維分解 / モルモット / ラット / オオミミマウス / in vitroセルロ-ス分解 / デグ- / ヌ-トリア / 消化管内容物滞留時間 / 繊維消化率
Research Abstract

Digestive efficiencies of fiber components and retention time of digesta in the digestive tract were measured in the mara, nutria, rabbit, guinea pig, rat, degu, hamster and leaf-eared mouse on same diet containing 50 % alfalfa.
Considerable linear relation is observed between body weights and retention times of digesta. However, the relation between the digestibility of ADF and the mean retention time of a particle marker in the eight animals showed no clear relation among these animals. From these results, the retention time and transit time of digesta in the whole tract is inadequate to explain the difference in the ability of fiber digestion in these animals.
The number of caecal cellulolytic bacteria and in vitro cellulolytic activity of caecal microbes in the guinea-pig, rat and leaf-eared mouse were determined by the roll tube method (Hungate 1966) and by inoculating the paper-containing media from dilutions of caecal contents (Mann 1968), respectively.
The number of cellulolytic bacteria was smaller in the guinea-pig than in the rat and leaf-eared mouse. The cellulolytic activity of caecal microbes was detected in the medium containing rumen fluid in the guinea-pig. The activity was detected both in the medium containing rumen fluid and in the medium without rumen fluid only in the rat.
These suggest that the species and activity of caecal microbes are different between each animal species fed on the same diet, and that the digestibility of fiber is not depend on the activity of microbes in the caecum. The digesta retention time and pattern of digesta flow in the large intestine can be considered to be the main factor to affect fiber digestion in the hindgut fermenters

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] SAKAGUCHI E.,NIPPASHI K.and ENDOH G.: "Digesta retention and fibre digestion in maras and guinea pigs." Comparative Biochemistry and Physiology.101A. 867-870 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SAKAGUCHI E.,KAIZU K. and NAKAMICHI M.: "Fibre digestion and digesta retention on different physical forms of the feed in the rabbit." Comparative Biochemistry and Physiology.102A. 559-563 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SAKAGUCHI E. and NABATA A.: "Comparison of fibre digestionj and digesta retention time between nutrias (Myocaster coypus) and guinea-pigs (Cavia porcellus)." Comparative Biochemistry and Physiology.103A. 601-604 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SAKAGUCHI E. and OHMURA S.: "Fibre digestion and digesta retention in guinea pigs (Cavia porcellus), degus (Octodon degus) and leaf-eared mice (Phyllotis darwini)." Comparative Biochemistry and Physiology.103A. 787-791 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] SAKAGUCHI E.: "Comparative aspects of fibre digestion and digesta transit in caecum fermenters.In Hindgut'91(Sakata Ed.)" Senshu Univ.Press., 135 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakaguchi E.: "Comparative aspects of fiber digestion and digesta transit in caecum fermenters." In : Hindgut'91 (Sakata Ed.) Senshu Univ. Press.49-69 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakaguchi E., Nippashi K. and Endoh G.: "Digesta retention and fiber digestion in maras and guinea pigs." Comp. Biochem. Physiol.101A(4). 867-870 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakaguchi E., Kaizu K. and Nakamichi M.: "Fiber digestion and digesta retention on different physical forms of the feed in the rabbit." Comp. Biochem. Physiol.102A(3). 559-563 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakaguchi E. and Nabata A.: "Comparison of fiber digestionj and digesta retention time between nutrias (Myocaster coypus) and guinea-pigs (Cavia porcellus)." Comp. Biochem. Physiol.103A(3). 601-604 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sakaguchi E. and Ohmura S.: "Fiber digestion and digesta retention in guinea pigs (Cavia porcellus), degus (Octodon degus) and leaf-eared mice (Phyllotis darwini)." Comp. Biochem. Physiol.103A(4). 787-791 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 坂口 英 大村 昌治郎: "モルモット,ラット,オオミミマウスの盲腸内セルロース分解菌数およびin vitroセルロース分解活性" 草食家蓄用実験動物. 18. (1993)

    • Related Report
      1992 Annual Research Report
  • [Publications] Sakaguchi E.,Nippashi K.and Endoh G.: "Digesta retention and fibre digestion im maras (Dolichotis patagonum) and guineaーpigs." Comparative Biochemistry and Physiology,A.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakagchi E.,Kaizu K.and Nakamichi M.: "Fibre digestion and digesta retention on diferent physical forms of the feed in the rabbit." Comparative Biochemistry and Physiology,A.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakaguchi E.and Nabata A.: "Comparison of fibre digestion and digesta retention time between nutrias and guinea pigs." Comparative Biochemistry and Physiology,A.

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakaguchi E.and Ohmura S.: "Fibre digestion and digesta retention in leafーeared mice,degus and guineaーpigs." Comparative Biochemistry and Physiology,A.

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakaguchi,E.: "Comparison of fibre digestion and digesta retention time between the leafーeared mouse,degu,guinea pig and nutria." Comparative Biochemistry and Physiology.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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