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Investigation of nephritogenicity using murine model of IgA nephrop

Research Project

Project/Area Number 02670279
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MUSO Eri  Kyoto University, Faculty of Medicine, Instructor, 医学部, 助手 (10190852)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Eiji  Kyoto Univ. Medicine, Instruct, 医学部, 助手 (80179605)
YOSHIDA Haruyoshi  Kyoto Univ. Medicine, professor, 医学部, 助教授 (80135574)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1991: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsIgA nephropathy / Monoclonal IgA / gq70 / chronic glomerulonephritis / day mouse / ddYマウス / gP70 / 多量体IgA / DBA_2マウス
Research Abstract

In order to elucidate the pathogenic characteristics of IgA in the development of mesangial proliferative glomerulonephritis (GN) in IgA nephropathy, the establishment and analysis of monoclonal IgA from a murine model of glomerulonephritis with IgA deposition were tried. The results obtained were as follows:
1) We established 16 monoclonal IgA (mIgA) clones by five fusions between spleen cells from 60 week old ddy mice which showed severe mesangial proliferative GN with IgA deposition and nonsecreting Balb/c myeloma cell (P3x63-Ag8653).
2) The investigation of molecular size of these mIgA revealed that there was no monomeric IgA but only dimeric or polymeric IgA were detected. The pIs of these mIgA which were investigated by agarose gel isoelectric focusing analysis ranged from 4.0-6.0. Those results supported our previous data of serum and kidney elluate polyclonal IgA which were dimeric or polymeric size with more acid electric charge.
3) The investigation of specific antibody activity revealed that one of these mIgAs showed anti-DNP activity although the affinity to the antigen was not enoughly high by competitive ELIZA.
4) Two clones showed antibody activity against the retroviral gp70. The trials to provoke GN by intra-peritoneal injection of these mIaAs to DBA2 mice did not succeed because of insufficient quantity of IgA injected. Further injections are tried.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Eri Muso,Haruyoshi Yoshida Eiji Takeuchi ea al: "Pathogenic role of polyclonal and polymeric IgA in a murine model of mesangial proliferative glomerulonephritis with IgA deposition" Clinical and experimental immunology. 84. 459-465 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Eri muso,Haruyoshi Yoshida,Eiji Takeuchi et al.: "Charge and Molecular size of monoclonal IgA derived from ddy mice,a murine model of IgA nephropathy" The XIth International Congress of Nephrology(July 15th-20th,1990 Tokyo).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Eri Muso, Haruyoshi Yoshida, Eiji Takeuchi, Toshihide Shimada, Masatomo Yashiro, Taketoshi Sugiyama: "Pathogenic role of polyclonal and polymeric IgA in a murine model of mesangial proliferative glomerulonephritis with IgA deposition" Clinical and Experimental Immunology. 84. 459-465 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Eri Muso, Haruyoshi Yoshida, Eiji Takeuchi Toshihide Shimada, Masatomo Yashiro, Chuichi Kawai: "Charge and molecular size of monoclonal IgA derived from ddy mice, A murine model of IgA nephropathy." The XIth International Congress of Nephrology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Eri Muso,Haruyoshi Yoshida,Eiji Takeuchi Et al.: "Pathogenic role of polyclonal and polymeirc IgA in a murine model of mesangial proliferative glomerulonephritis with IgA deposition" Clinical and experimental immunology. 84. 459-465 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Eri Muso,Haruyoshi Yoshida,Eiji Takeuchi et al.: "Charge and molecular size of monoclonal IgA(mIgA) derived from ddY mice.A murine model of IgA nephropathy." XIth Internal Congress of Nephrology(一般演題)(平成2年7月15日ー20日).

    • Related Report
      1991 Annual Research Report
  • [Publications] 武曽 恵理: "Pathogenic role of polyclonal and multimeric IgA in a murine model of mesangial proliferative glomerulonephritis with IgA deposition" Clinical and Experimental Immunology.

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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