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Studies on glutathione S-transferase-pi

Research Project

Project/Area Number 02670327
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionToho University

Principal Investigator

SUGIMOTO Motonobu  Toho Uuiv.Sch.of Med., Ass.Prof., 医学部, 助教授 (30120281)

Co-Investigator(Kenkyū-buntansha) SAIKI Hideki  Toho Uuiv.Sch.of Med., Assistant, 医学部, 助手
SATO Takashi  Toho Uuiv.Sch.of Med., Assistant, 医学部, 助手
YAMAMURO Wataru  Toho Uuiv.Sch.of Med., Assistant, 医学部, 助手 (80166777)
ITO Kinji  Toho Uuiv.Sch.of Med., Lecturer, 医学部, 講師 (40057758)
Project Period (FY) 1990 – 1992
Project Status Completed (Fiscal Year 1992)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
KeywordsGlutathione S-transferase / Isoenzyme / Precancerous lesion / Primary cultured hepatocytes / Hepatocarcinogenesis / Immunohistochemistry / グルタチオン S‐トランスフェラーゼ / 肝前癌病変 / グルタチオン Sートランスフェラ-ゼ / 実験肝癌 / グルタチオンSートランスフェラ-ゼ / 肝悪性腫瘍 / 培養肝細胞
Research Abstract

1)The immunohistochemical study was carried out to elucidate the localization of glutathione S-transferase (GST) isozymes in primary liver tumors : 28 lesions of hepatocellular carcinoma (HCC) and 10 cases of cholangiocellular carcinoma (CCC) ; and tumor-like lesions : 2 cases of focal nodular hyperplasia, 5 cases of adenomatous hyperplasia (AH) and 4 cases of licer cell dysplasia. In HCC, GST-alpha positivity was 60%, but GST-pi was negative in all. In CCC. alpha was negative in all, but pi positivity was 80%. In tumor-like lesions, alpha was positive in all, but pi was positive in only 2 cases of AH. pi was suggested to be significant as a marker of CCC. Moreover, since pi was present in some AH, it was suggested that AH might be a precancerous lesion.
2)The changes in GST activity and GST subunit components in primary cultured rat hepatocytes were studied. Enzyme activity decreased at 48hr and subsequently increased and returned by 120hr to levels initially observed at 12hr. lmmunocy … More tochemical study was performed with 3 polyclonal antibodies : anti-Ya, Yb_1 and Yp. With anti-Ya, hepatocytes were persistently positive up to 144hr. With anti-Yb_1, hepatocytes were positive at 24hr, though positivity then gradually decreased. On the other hand, with anti-Yp, cells were almost negative at 48hr and became obviously positive at 96hr. Overall time-dependent increase in the GST activity can be accounted for by increase of Yp subunit.
3) In order to clarify an implication of expression of GST-P (pi-class, Yp-sudunit) in the hepatocarcinogenesis, immunohistochemical study was performed on two rat groups : A-group with continual administration of diethylnitrosamine (DEN) ; B-group with 4 weeks administration of DEN and 8 weeks withdrawal. In A-group, GST-P positive cells were scattered on the 4th day and then minifoci and large foci appeared. From the 6th week hyperplastic nodules with positive GST-P were completed and from the 10th weeks cancer lesions with faint GST-P were recognized. In B-group, GST-P positive large foci changed to minifoci and single cell, and hyperplastic nodules decreased in number disappearing on the 8th week. Precancerous GST-P positive cell was suggested to be reversible. Less

Report

(4 results)
  • 1992 Annual Research Report   Final Research Report Summary
  • 1991 Annual Research Report
  • 1990 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 佐藤 隆: "肝悪性腫瘍および腫瘍類似病変におけるglutathione S-transferaseアイソザイムの免疫組織化学的検討" 肝臓. 33. 390-398 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Asakura I.,et al: "Glutathione S-transferases in primary cultured rat hepatocytes" Gastroenterologia Japonica. 28. 34-45 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] 永井 一毅: "ラット肝化学発癌過程におけるglutathione S-transferase-Pの発現" 肝臓.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Sato T: "Immunohistochemical localization of human glutathione S-transferase isozymes in primary liver tumors and intrahepatic tumor-like lesions" Acta Hepatol Jpn. 33. 30-38 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Asakura I,et al: "Glutathione S-transferases in primary cultured rat hepatocytes" Gastroenterol Jpn. 28. 34-45 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary
  • [Publications] Nagai K: "The expression of glutathione S-transferase-P in the rat chemical hepatocarcinogenesis" Acta Hepatol Jpn.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1992 Final Research Report Summary

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Published: 1990-04-01   Modified: 2016-04-21  

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