| Project/Area Number |
02670469
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KOIZUMI Hiroko HOKKAIDO UNIVERSITY MEDICAL HOSPITAL, DERMATOLOGY, LECTURER, 医学部附属病院, 講師 (60113552)
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| Co-Investigator(Kenkyū-buntansha) |
YASUI Chikako HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, DERMATOLOGY, ASSISTANT, 医学部, 助手 (50210240)
FUKAYA Toru HOKKAIDO UNIVERSITY SCHOOL OF MEDICINE, DERMATOLOGY, ASSISTANT, 医学部, 助手 (90133727)
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| Project Period (FY) |
1990 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1992: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | KERATINOCYTE / CALCIUM / PEROXIDE / SUBSTANCE P / PHOSPHOLIPASE C / PROTEIN KINASE C / ADENYLATE CYCLASE / PSORIASIS / Protein kinase C / phospholipase C / protein kinase C / adenylate cyclase / 細胞内遊離カルシウム / リポコルチン / フォドリン / エピネフリン |
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| Research Abstract |
Epidermal keratinocytes proliferate and differentiate to make stratum corneum. Many chemical mediators, hormones and peptides stimulate keratinocytes to induce phosphophatidylinositol metabolism, alteration of intracellular free calcium, adenylate cyclase response activation. We elucidated that bradykinin, thrombin, substance P, beta-adrenarin, adenosine, and histamine induce increase of intracellular free calcium. These effects are mediated with synthesis of inositol 1,4,5-trisphosphate or adenylate cyclase activation. The importance of calcium as a second messenger is shown. Presence of membrane skeleton proteins such as spectrin, b-fodrin, 4.1 protein like proteins were detected immunologically and the alteration of their distribution in cultured keratinocytes were observed with the change of calcium concentration of the medium. A new technique was reported to detect peroxide produced from keratinocytes using dihydrorhodamine 123.
Cross-talk among transmembrane signaling systems in keratinocytes, and drugs to influence these systems should be investigated. Abnormal keratinization and acceleration of proliferation are considered to closely realt to pathogenesis of psoriasis. The alteration of membrane signaling systems in psoriasis and the action mechanisms of the drugs used in therapy of skin diseases are under investigation.
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