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Study on pathophysiology and therapy of serious organo-phosphate poisoning.

Research Project

Project/Area Number 02670685
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 麻酔学
Research InstitutionKagawa Medical School

Principal Investigator

SHIRAKAWA Yoichi  Kagawa Medical School, Hospital, Dep. of Emergency Med., Associate Professor, 医学部附属病院, 助教授 (90134600)

Co-Investigator(Kenkyū-buntansha) SEKI Keisuke  Kagawa Medical School Hospital, Dep. of Emergency Med., A, 医学部附属病院, 助手 (20226632)
TUKAMOTO Ikuko  Kagawa Medical School, Dep. of Anesthesiology, Assistant, 医学部, 助手 (10183477)
AMENO Kiyoshi  Kagawa Medical School, Dep. of Forensic Med., Associate Professor, 医学部, 助教授 (50019626)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsorgano-phosphate / poisoning / cholinesterase / acetylcholinesterase / pharmacokinetics / 薬物濃度 / 薬物血中濃度
Research Abstract

1. We confirmed a modified technique for the simultaneous determination of fenitrothion and its metabolite, 3-methyl-4-nitrophenol(MNP), in serum and urine by high performance liquid chromatography(HPLC)after Sep-PakC18 column extraction. Recovery yield of sumithion and MNP after Sep-PakC18 column extraction was 92.1<plus-minus>3.0 % and 90.0<plus-minus>2.9% in serum and 73.5 <plus-minus>4.3% and 88.2<plus-minus>1.7% in urine, respectively. Each linear calibration curve for sumithion and MNP was obtained in the range of 1-100 mug/ml. Quantitation was performed using the values of peak arearatio of fenitrothion or MNP to IS. Serial determination of both fenitrothion and MNP in acute poisoning patients exhibited that some therapeutic interventions might have an effect on the metabolism of fenitrothion
2. Kinetic analysis was used to study the time course of plasma cholinesterase(ChE)and erythrocyte acethylcholinesterase(RBC AChE)activities in patients of organophosphorus pesticides poisoning. The recovery of both enzymes increased exponentially and approached asymptotes, to which a simple one-compartment model was successfully fitted. The half-Life for this recovery period, the mean of which was 11.3 days for plasma ChE and 26.7 days for RBC AChE, was related neither to the severity of clinical symptomes nor to the chemical structure of the pesticides. The results indicated that(1)newly formed enzyme/erythre is a leading factor in the recovery, (2)the half-life of recovery is not a good indicator of the clinical course, and(3)dynamics of enzyme inhibition are different among compounds.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] 白川 洋一: "有機リン剤中毒に血液浄化法は有効か?" 医学のあゆみ. 155. 457-457 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 白川 洋一: "有機リン中毒症例における血漿コリンエステラ-ゼおよび赤血球アセチルコリンエステラ-ゼ回復過程の速度論的解析" 日本救急医学会雑誌. 2. 621-629 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 植木 正明: "臭化ジスチグミン中毒の1症例" 中毒研究. 4. 383-385 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kiyoshi Ameno: "Regional brain distribution of toluene in rats and in human autopsy." Arch.Toxicol.66. 153-156 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 福家 千昭: "HPLCによるスミチオンおよび主代謝産物3ーメチルー4ーニトロフェノ-ルの同時分析に関する研究と実際例への応用" 法医学の実際と研究.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoichi Shirakawa, Ikuko Tsukamoto, Keisuke Seki, Mayuki Aibiki, Kiyosi Ameno: "Kinetic Study on the Recovery of Plasma Cholinesterase and Erythrocyte Acetylcholinesterase Activities after Organophosphorus Poisoning in Humans." Journal of Japanese Association for Acute Medicine. 2(3). 621-629 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 白川 洋一: "有機リン中毒症例における血漿コリンエステラ-ゼおよび赤血球アセチルコリンエステラ-ゼ回復過程の速度論的解析" 日本救急医学会雑誌. 2. 621-629 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kiyoshi Ameno: "Regional brain distribution of toluene in rats and in a human autopsy" Archives of Toxicology. 66. 153-156 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 福家 千昭: "HPLCによるスミチオンおよび主代謝産物3ーメチルー4ーニトロフェノ-ルの同時分析に関する研究と実際例への応用" 法医学の実際と研究.

    • Related Report
      1991 Annual Research Report
  • [Publications] 白川 洋一: "有機リン中毒症例における血漿コリンエステラ-ゼおよび赤血球アセチルコンリエステラ-ゼ回復過程の速度論的解析" 日本救急医学会雑誌. 2. (1991)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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