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Analysis of structure of mucin type glycoprotein seaeted from endometrial Carcinoma

Research Project

Project/Area Number 02670755
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionHyogo Medical College

Principal Investigator

TSUJI Yoshiyuki  Hyogo Medical College, lecturer, 医学部, 講師 (60148658)

Co-Investigator(Kenkyū-buntansha) YAMASAKI Noriyuki  Hyogo Medical College, Assistant, 医学部, 助手 (50174644)
KOMORI Shinji  Hyogo Medical College, Assistant, 医学部, 助手 (60195865)
Project Period (FY) 1990 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsMucin / Carbohyhdrate / Monoclonal Antibody / endometrial Carcinoma / 糖鎖抗原 / 血液型物質 / 腫瘍マ-カ-
Research Abstract

Endometrial carcinoma cells produce a high molecular weight mucin type glycoprotein (MGP). This MGP was detected in sera of patients suffering from endometrial carcinoma. We investigated the structure of MGP secreted from endometrial carcinoma culture cell line.
Semi-purification showed that MGP was high molecular material and there were no terminal sialic acids. The carbohydrate structure was analysed by various monoclonal antibodies which specifically react to previous defined carbohydrate structures, and peptide portion was investigated by Edman's amino sequence analysis after high purification procedures by high performance liquid chromatography and immunoaffinity column. It was elucidated that MGP was composed of highly fucosylated poly lactosamine carbohydrate structure and core peptide of approximately MW. 40K dalton.
There were no homology peptide structures registered in peptides EMBO data base but the putative DNA sequence of the amino sequence had high homology with pancreatic elastase III.
This results suggest that MGP partially share an ancient gene with pancreatic elastase III.

Report

(3 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] Yoshiyuki Tsuji: "Hereditary Colorectal Cancer" Springer-Verlag, 575 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshiyuki Tsuji (J. Utsunomiya et al Edited): Hereditary Colorectal Carcinoma : A Monoclonal Antibody, C12 Against Endometial Carcinoma, Recognize Poly-fuosyl Lactosamine-Bearing Glycoprotein. Springe-Verlog,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshiyuki Tsuji: "Hereditary Colorectal Cancer “A Monoclonal Antibady CR.Against Endometial Carcuoma Recognizing Polyfacosyl Lactosanineーbeniyglyueprotion" SpringerーVerlag, 572 (1990)

    • Related Report
      1990 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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