Stable production of recombinant human sperm immobilizing antibody and its Fab fragment using cDNA expression vectors
| Project/Area Number |
02671043
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
医学一般
|
|---|
| Research Institution | Hyogo Medical College |
|---|
Principal Investigator |
YAMASAKI Noriyuki Hyogo Medical College, Dept. Obstetrics & Gynecology, Assistant, 医学部, 助手 (50174644)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KOMORI Shinji Hyogo Medical College, Dept. Obstetrics & Gynecology, Assistant, 医学部, 助手 (60195865)
SAWAI Hideaki Hyogo Medical College, Dept. Obstetrics & Gynecology, Assistant, 医学部, 助手 (80215904)
|
|---|
| Project Period (FY) |
1990 – 1991
|
| Project Status |
Completed (Fiscal Year 1991)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1991: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
|
|---|
| Keywords | sperm immobilizing antibody / recombinant antibody / cDNA expression / gene transfection / BPV vector |
|---|
| Research Abstract |
Sperm immobilization antibodies present in sterile women are one of theprincipal immunological causes of infertility. To identify the antigenseliciting these antibodies, we have constructed cell lines that stably secrete recombinant human sperm-immobilizing antibody using cDNA expression vectors. For production of the antibody, cDNAs of the heavy and light chain immunoglobulingenes were cloned from the class-switched (from IgM to IgG) human sperm-immobilizing antibody En46 and inserted into modified bovine papilloma virus-based cDNAexpression vectors BCMGSHyg and BCMGSNeo, respectively. These constructs were sequentially transfected into the mouse myeloma cell line X63Ag8.653 by electro-poration. The established transfectants produced recombinant antibody that retained human sperm-immobilizing activity in non-selective medium for more thanone month. The use of this genetically engineered antibody should contribute to the understanding and treatment of immunological infertility and to the development of a contraceptive vaccine.
|
Report
(3 results)
Research Products
(2 results)
