Immunological Activity of Water-solubilized Lignins and its Action Mechanism
Grant-in-Aid for Scientific Research (C).
|Research Institution||The University of Tokyo|
YAMAZAKI Sunao Univ. of Tokyo, Fac. of Agric., Professor, 農学部, 教授 (00011982)
TODA Shozo Univ. of Tokyo, Fac. of Agric., Professor emeritus, 農学部, 教授 (40011845)
IIYAMA Kenji Univ. of Tokyo, Fac, of Agric, Assist. Prof., 農学部, 助手 (60012077)
YOSHIMURA Etsuro Univ. of Tokyo, Fac. of Agric., Assist. Prof., 農学部, 助手 (10130303)
OKUBO Akira Univ. of Tokyo, Fac. of Agric., Assoc. Professor, 農学部, 助教授 (20111479)
|Project Fiscal Year
1990 – 1991
Completed(Fiscal Year 1991)
|Budget Amount *help
¥1,800,000 (Direct Cost : ¥1,800,000)
Fiscal Year 1991 : ¥400,000 (Direct Cost : ¥400,000)
Fiscal Year 1990 : ¥1,400,000 (Direct Cost : ¥1,400,000)
|Keywords||water-solubilized lignins / high molecular polyanions / macrophages, activation of / immunoactive lignins / bone marrow cells, proliferation of / autoradiography / imaging plate method / xenobiotic metabolism / 水溶性リグニン / 高分子ポリアニオン / マクロファ-ジ活性化 / 免疫活性物質 / 骨髄細胞増殖促進 / 吸収排泄 / イメ-ジングプレ-ト / 薬物代謝 / 骨髄細胞増殖促進活性|
(1)Structure and activity relationship has been studied among the water-soluble lignins such as LENderived EP3, lignosulfonates(LS), and their derivatives or decomposition products. For both the activation of reurine macrophages and the proliferation of bone marrow cells, polyanionic nature of lignin and its derivatives having the molecular weight more than 10, 000 is the first requisite to emerge the activities. No difference in activities was observed between dissociation groups, such as carboxyl, solfonyl or sulfuryl group in lignins.
(2)Three water-solubilized lignins with different molecular weight and number of dissociation groups were hydroxymethylated with ^<14>C-formaldehyde. The activities were maintained after derivatization. They were administered orally and intravenously to rats and time-course distribution of radio, activity was measured both by autoradiography using the imaging plate method and ordinally tissue analysis. As a result, the lignins were absorbed smoothly via
blood and deposited in the tissues mainly in liver, bone marrow, kidney. The greater part of the radioactivity excreted rapidly into urine, the smaller part, however, remained in the above hislily productive organs for the longer period, suggesting that it revealed the good correlation with the results obtained from those of activity assays.
(3)EP3, LS, dextran sulfate, polystyrene sulfonate, etc., induced not only the morphological changes of macrophages, but also augmented the glucose consumption. Activation of macrophages was found to depend on polyanionic nature of water-solubilized lignins.
(4)Water-solubilized lignins enhanced more strongly the incorporation of ^3H-thymidine into bone marrow cells than imunoactive LPS. Dextran sulfate did not show any activity. Among the bone marrow. cells, it was shown that progenitor macrophage cells were most strongly proliferated by water-soluilized lignins.
Research Output (6results)