| Project/Area Number | 03041078 |
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Field Research |
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| Research Institution | Nihon University |
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Principal Investigator |
SHIKATA Toshio Nihon Univ., Medicine Professor, 医学部, 教授 (50009932)
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| Co-Investigator(Kenkyū-buntansha) |
AYE Thein Thein B.M.R.
SHROSTHA S.M. Bir Hospital, 内科部長
荘 輝 北京医科大学, 医学部, 教授
INABA Yutaka Juntendo Univ., Medicine Professor, 医学部, 教授 (30010094)
SUGITANI Masahiko Nihon Univ., Medicine Lecturer, 医学部, 講師 (40187654)
ESUMI Mariko Nihon Univ., Medicine Associate Professor, 医学部, 助教授 (60160363)
UCHIDA Toshikazu Nihon Univ., Medicine Associate Professor, 医学部, 助教授 (80060078)
HUI Zhuang Beijing Medical College Professor
THEIN Thein Department of Medical Research, Myanmar, 研究員
S.M.SHRESTHA Bin Hospital, 部長
CAO Kueーyi 新彊衛生防疫站所長, 所長
CAO Xueーyi 新彊衛生防疫站, 所長
西園 晃 日本大学, 医学部, 助手 (70218155)
鈴木 高祐 日本大学, 医学部, 助教授 (00158974)
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| Project Period (FY) |
1991 – 1993
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| Project Status |
Completed(Fiscal Year 1993)
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| Budget Amount *help |
¥16,000,000 (Direct Cost : ¥16,000,000)
Fiscal Year 1993 : ¥5,000,000 (Direct Cost : ¥5,000,000)
Fiscal Year 1992 : ¥5,000,000 (Direct Cost : ¥5,000,000)
Fiscal Year 1991 : ¥6,000,000 (Direct Cost : ¥6,000,000)
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| Keywords | Hepatitis E / Virus genome / Animal model / Vaccine / anti-HEV / ウイルス性肝炎流行 / 散発性急性肝炎 / E型肝炎ウイルス遺伝子 / 抗体アッセイ系 / ウイルス遺伝子 |
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| Research Abstract |
Hepatitis E is similer to hepatitis A. Hepatitis E virus (HEV) is transmitted by fecal-oral route and only cause acute hepatitis. This hepatitis E is mainly observed in South-Asia, such as India, Nepal, Myanmar, Uiguru Autonomous Region of China. Attempt was made to 1. establish an animal model, 2. identify a causative virus, 3. molecular clone of HEV genomne, 4. establish an assay kit for diagnosis and sero-epidemiological study of hepatitis E. 5. Vaccine development.
If we inoculate enough amount virus intravenously into rhesus monkeys we could produce acute hepatitis in animals. Also we could get large amount virus from the bile of the infected animals. We constructed lambda gt11 cDNA library from the bile and immunoscreened HEVRNA. We got 4 clones from 3'terminal and finally we sequenced whole genome of HEV. We also compared sequence of Burmese clone and Chinese clone. Homology was 93% in nucleic acid. We established assay kit for anti-HEV using peptide of 3'terminal region. We screened acute sporadic hepatitis in Japan, China and Miyanmar. We found in Japan, we observed very few imported cases of hepatitis E. However, in China, even Beijing and Shanghai, almost 10% of acute sporadic hepatitis cases were caused by HEV. We did efficacy experiment of recombinant vaccine using monkeys. However, we failed to protect HEV infection by our vaccine.
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