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Seroepidemiological studies on hepatitis E and development of vaccine.

Research Project

Project/Area Number 03041078
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionField Research
Research InstitutionNihon University

Principal Investigator

SHIKATA Toshio  Nihon Univ., Medicine Professor, 医学部, 教授 (50009932)

Co-Investigator(Kenkyū-buntansha) AYE Thein Thein  B.M.R.
SHROSTHA S.M.  Bir Hospital, 内科部長
荘 輝  北京医科大学, 医学部, 教授
INABA Yutaka  Juntendo Univ., Medicine Professor, 医学部, 教授 (30010094)
SUGITANI Masahiko  Nihon Univ., Medicine Lecturer, 医学部, 講師 (40187654)
ESUMI Mariko  Nihon Univ., Medicine Associate Professor, 医学部, 助教授 (60160363)
UCHIDA Toshikazu  Nihon Univ., Medicine Associate Professor, 医学部, 助教授 (80060078)
HUI Zhuang  Beijing Medical College Professor
THEIN Thein  Department of Medical Research, Myanmar, 研究員
S.M.SHRESTHA  Bin Hospital, 部長
CAO Kueーyi  新彊衛生防疫站所長, 所長
CAO Xueーyi  新彊衛生防疫站, 所長
西園 晃  日本大学, 医学部, 助手 (70218155)
鈴木 高祐  日本大学, 医学部, 助教授 (00158974)
Project Period (FY) 1991 – 1993
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥16,000,000 (Direct Cost: ¥16,000,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1992: ¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1991: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsHepatitis E / Virus genome / Animal model / Vaccine / anti-HEV / ウイルス性肝炎流行 / 散発性急性肝炎 / E型肝炎ウイルス遺伝子 / 抗体アッセイ系 / ウイルス遺伝子
Research Abstract

Hepatitis E is similer to hepatitis A. Hepatitis E virus (HEV) is transmitted by fecal-oral route and only cause acute hepatitis. This hepatitis E is mainly observed in South-Asia, such as India, Nepal, Myanmar, Uiguru Autonomous Region of China. Attempt was made to 1. establish an animal model, 2. identify a causative virus, 3. molecular clone of HEV genomne, 4. establish an assay kit for diagnosis and sero-epidemiological study of hepatitis E. 5. Vaccine development.
If we inoculate enough amount virus intravenously into rhesus monkeys we could produce acute hepatitis in animals. Also we could get large amount virus from the bile of the infected animals. We constructed lambda gt11 cDNA library from the bile and immunoscreened HEVRNA. We got 4 clones from 3'terminal and finally we sequenced whole genome of HEV. We also compared sequence of Burmese clone and Chinese clone. Homology was 93% in nucleic acid. We established assay kit for anti-HEV using peptide of 3'terminal region. We screened acute sporadic hepatitis in Japan, China and Miyanmar. We found in Japan, we observed very few imported cases of hepatitis E. However, in China, even Beijing and Shanghai, almost 10% of acute sporadic hepatitis cases were caused by HEV. We did efficacy experiment of recombinant vaccine using monkeys. However, we failed to protect HEV infection by our vaccine.

Report

(3 results)
  • 1993 Final Research Report Summary
  • 1992 Annual Research Report
  • 1991 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Uchida,T: "An epidemic outbreak of hepatitis E in Yangon of Myanmar:antibody assay and animal transmission of the virus." J.of Hepatology. 18. 369-372 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Uchida,T: "An epidemic outbreak of hepatitis E in Yangon of Myanmar : antibody assay and animal transmission of the virus." J. of Hepatology. 18. 369-372 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] T.Uchida: "Hepatitis E virus c DNA cloningand expression." Microbiol.Immunol.36. 67-72 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.T.Aye: "Complete nucleotide sequence of hepatitis E virus isolated from the Xinjiang epidemic(1986-1988)of China." Nucleic Acids Research. 20. 3512 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Uchida: "Hepatitis E" Gastroenterologia Japonica. 27. 687-697 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Kato: "Interferon-inducible Gene expression in chimpanzee liver infected with hepatitis C virus." Virology. 190. 856-860 (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] T.Shikata et al.: "Primary liver cancer in Japan." Springer-Verlag, (1992)

    • Related Report
      1992 Annual Research Report
  • [Publications] 杉本 洋之: "HCV抗原をコ-ドする遺伝子断片C8ー2を用いた抗体アッケイ系" 肝臓. 32. 436-437 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 林 仲信: "C型肝炎ウイルス" 医学と薬学. 25. 1237-1248 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 江角 真理子: "C型肝炎ウイルスコア抗体アッセイ系JCC(CP120)" 肝胆膵. 22. 897-900 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 志芳 俊夫: "Viral Hepatitis C,D and E" Exeepta medica, 405 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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