MURATA Jin Lecturer, Faculty of Science, University of Tokyo, 理学部, 講師 (90134452)
YAMAMOTO Hirobumi Research Associate, Gifu Pharmaceutical University, 助手 (40210554)
TANAKA Toshiyuki Research Associate, Gifu Pharmaceutical University, 助手 (60137065)
IINUMA Munekazu Associate Professor, Gifu Pharmaceutical University, 助教授 (70082998)
MIZUNO Mizuo Professor, Gifu Pharmaceutical University, 教授 (00082967)
FRANK A.LANG Southern Oregon College, Professor
MURATA Hiroko Research Associate, Faculty of Pharmaceutical Sciences, Setsunan University, 薬学部, 助手 (20167620)
INADA Akira Associate Professor, Faculty of Pharmaceutical Sciences, Setsunan University, 薬学部, 助教授 (70095305)
LANG Frank A. Professor, Department of Botany, Southern Oregon State College
LANG Frank A Southern Oregon State College, Professor
|Budget Amount *help
¥9,000,000 (Direct Cost : ¥9,000,000)
Fiscal Year 1992 : ¥4,500,000 (Direct Cost : ¥4,500,000)
Fiscal Year 1991 : ¥4,500,000 (Direct Cost : ¥4,500,000)
Acetone, ethanol, and/or 70% aqueous ethanol extracts of 3 lichens and 83 species of higher plants (82 Spermatophytes and a Pteridophyte) (containing 46 species of plants with surface exudates), collected from SW Oregon and NW California in Summer of 1991, were tested for their inhibitory effects on HIV-1 reverse transcriptase (RT), their inhibitory effects on the Epstein-Barr virus (EBV) activation, and their cytotoxic activities against cultured P-388 cancer cells, respectively.
Significant inhibitory effects on HIV-1 RT were observed in 46 species and especially, ethanol extracts of polygonum majos, Purshia tridentata, Ceanothus integerrimus, Grindelia nana and other 2 species showed more potent inhibition than 20% at 0.5 mug/ml concentration. Furthermore, beta-sitosterol glucoside (IC_<50>=1.8 mug/ml) isolated from Purshia tridentata, and ursolic and betulinic acids (IC_<50>=1.4 and 0.8 mug/ml,respectively) from Amelanchier alnifolia var. pumila showed potent inhibition on HIV-1 RT.
Acetone extracts of 15 species (Eriodictyon californicum and others) and ethanol extracts of 47 species (Sophora leachiana and others) showed significant inhibition on the EBV activation. Furthermore, 3 flavanones [5,7-(OH)_2 flavanone, etc.] isolated from E. californicum and 4 flavonols (ermarin, etc.) from Cordylanthus viscidus gave potent inhibition on the EBV activation.
Cytotoxic activities against P-388 cells were examined in 115 extracts (57 species) which are giving no desirable results at present.
Similarly, acetone, ethanol (or methanol), and/or 70% aqueous ethanol (or methanol) extracts of a lichen and 162 species of higher plants (160 Spermatophytes and 2 Pteridophytes), collected from SE Oregon in Summer of 1992 have been subjected to the before-mentioned three types of the biological tests. Up to date, it has been revealed that 9 extracts from Rosa woodsii, Ribes aureum, and other 3 species showed potent inhibitory activities on HIV-1 RT and 14 extracts from Pentagrama triangularis, Prunus emarginata and other 10 species, potent inhibitory effects on the EBV activation. Less