BLONDON F. フランスCNRS植物科学研究所, 主任研究員
QUETIER F. 南ハ゜リ大学(フランス), 教授
WEIL J. 植物分子生物学研究所(フランス), 教授
TEPFER M. INRA Versailles, France, 研究員
DELSENY M. University of Perpignan, France, 教授
RANJEVA R. ポールサバティエ大学(フランス), 教授
下村 講一郎 厚生省, 国立衛生試験所, 室長
SANGWAN R.S. University of Picardie, France, ピカルディー大学, 教授
佐藤 忍 筑波大学, 生物科学系, 講師 (70196236)
谷本 静史 佐賀大学, 農学部, 助教授 (20217152)
小野 道之 秋田県農業短大, 生物工学研究科, 講師 (50201405)
猪口 雅彦 岡山理科大, 理学部, 助手 (00213190)
石川 恵子 千葉大学, 園芸学部, 助手 (20212839)
野村 港二 筑波大学, 農林学系, 講師 (00183905)
京 正晴 香川大学, 農学部, 助教授 (70195395)
鎌田 博 筑波大学, 生物科学系, 教授 (00169608)
酒井 慎吾 筑波大学, 生物科学系, 助教授 (60033388)
藤井 正 筑波大学, 生物科学系, 教授 (20011611)
GUERN J フランス, CNRS植物科学研究所, 室長
DELSENY M フランス, ペルピニョン大学, 教授
OTTEN L フランス, 植物分子生物学研究所, 室長
SANGWAN R S フランス, ピカルディー大学, 教授
TEPFER M フランス, INRAベルサイユ研究所, 主任研究員
DELESENY M フランス, ペルピニョン大学, 教授
GUERN J. Institute des Sciences Vegetales, CNRS, France
FUJII Tadashi Institute of Biological Sciences, University of Tsukuba
SATOH Shinobu Institute of Biological Sciences, University of Tsukuba
NOMURA Kouji Institute of Agriculture and Forestry, University of Tsukuba
INOGUCHI Masahiko Department of Biochemistry, Okayama University of Science
SAKAI Shingo Institute of Biological Sciences, University of Tsukuba
OONO Michiyuki Biotechnology Institute, Akita Prefectural College of Agriculture
ISHIKAWA Keiko Faculty of Horticultue, Chiba University
KAMADA Hiroshi Institute of Biological Sciences, University of Tsukuba
|Budget Amount *help
¥11,500,000 (Direct Cost : ¥11,500,000)
Fiscal Year 1993 : ¥3,000,000 (Direct Cost : ¥3,000,000)
Fiscal Year 1992 : ¥3,500,000 (Direct Cost : ¥3,500,000)
Fiscal Year 1991 : ¥5,000,000 (Direct Cost : ¥5,000,000)
Cooperative research between Japan and France was carried out in plant bioscience field during 3 years.
1. On molecular mechanisms of somatic and zygotic embryogenesis : In Japan, embryogenic cell proteins (ECPs), which were found in embryogenic cells but not in non-embryogenic cells, were detected in carrot. cDNAs encoding some ECPs (ECP31, ECP40, and ECP63) were also isolated from carrot. In France, a partial cDNA clone homologous to ECP31 was isolated from a cDNA bank of immature seeds of Arabidopsis thaliana. To clarify physiological functions of ECPs in embryogenesis, exchanges of genes and information have been continued.
2. On signal transaction of phytohormones : In Japan, proteins binding to some phytohormones were isolated from hypocotyls of some plants. A Japanese researcher visited a French institute in 1992 and 1993 and cooperative research on isolation of auxin-binding proteins was done. By the work, an auxin-binding protein was partially purified from grape suspension cells and the purification has been continued.
3. On regulation mechanisms of plant gene expression : By the cooperative research between Japan and France, promoter regions of some T-DNA genes of Ti plasmids were isolated and ligated with GUS reporter gene. The chimerical genes were delivered into tobacco. In transgenic tobacco containing the GUS reporter gene with a promoter region of tmr gene (Ptmr-GUS), the GUS expression was not influenced by phytohorome treatments. Analyses of the expression of Ptmr-GUS in carrot somatic embryos have been carrying out.
4. On workshops between Japan and France : In 1991, a workshop between Japan and France in plant bioscience field had been held in France. Many researchers in both countries were attended in the workshop and discussed on their research and cooperative works. In 1993, similar workshop had been held in Japan.
Researchers in both countries reported the results of cooperative works and discussed on the future collaboration.