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進化的分子工学に基づく生体高分子の設計

Research Project

Project/Area Number 03353011
Research Category

Grant-in-Aid for Co-operative Research (B)

Allocation TypeSingle-year Grants
Research Field 物質生物化学
Research InstitutionTokyo Institute of Technology

Principal Investigator

大島 泰郎  東京工業大学, 生命理工学部, 教授 (60167301)

Co-Investigator(Kenkyū-buntansha) 今中 忠行  大阪大学, 工学部, 教授 (30029219)
岡田 弘輔  熊本工業大学, 工学部, 教授 (20028947)
伏見 譲  埼玉大学, 工学部, 教授 (80011641)
郷 通子  名古屋大学, 理学部, 教授 (70037290)
柳川 弘志  三菱化成, 生命科学研究所, 主任研究員
Project Period (FY) 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywords分子進化工学 / エクソンシャフリング / タンパク質工学 / タンパク質分子設計 / 進化の分子機構
Research Abstract

進化分子工学の推進について、以下の3つの点について検討した。その結果得られた結論と提言に基づいて、重点領域研究計画を立案し、平成5年度からの研究課題として提案した。
1研究の現状分析:進化分子工学がこれまでに挙げてきた研究成果について検討し、カバ-すべき研究課題について討論した。特に、研究課題を階層別に分けることにより研究の戦略目標と有効な研究手法の組合せを整理することが出来た。
2わが国における研究状況:分担者全員による研究報告を行い、わが国のこの分野の研究活動の特徴、弱点について意見交換を行った。
3早急に推進すべき課題:わが国の研究の特徴を生かし、進化分子工学のより効率的な推捗を計かるにはどの様な課題を緊急、かつ集注的に研究すべきかを討論した。その結果、「空間配列上の適応歩行」という概念を核として、自然選択実験系としては核酸レベル、核タンパク質レベル、細胞レベルの三つの階層について、変異の方法としては点突然変異とエクソンシャッフリングの二つの機構について集中的に研究することが必要と結論した。わが国の研究活動をレベルアップするために必要な研究チ-ムを策定した。
進化分子工学の分野により多くの若手研究者が参入するよう公開講演会「進化的分子工学こと始め」を開催し、この分野の将来展望について述べた。

Report

(1 results)
  • 1991 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Onodera,k.: "Crystallization and Preliminary X-ray Studies of Chimerie 3-Isoropylmalate Dehydrogenase between Bacillus subtilis and Thermus thermophilus HB8." J.Biochem.109. 1-2 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kirino,H.: "Molecular cloning and nucleotide sequence of 3-isopropylmalate dehydrogenase gene (leuB) from an extreme thermophile,Thermus aquaticus YT-1." J.Biochem.109. 852-857 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Xu,X.: "Necleotide sequence of the gene encoding NADH dehydrogenase from an alkalophile,Bacillus sp.strain YN-1." J.Biochem.109. 678-683 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Fujiyama,K.: "Characterization of Hybrid Enzymes between 6-Aminohexanoate-Dimer Hydrolase and Its Analogous Protein." J.Fermet.Bioeng.71. 298-302 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Imanaka,T.: "Design of temperature-sensitive penicillinase repressors by replacement of Pro in predicted β-turn structures." J.Bacteriol.174. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sakada.H.: "Cloning and sequencing of the gene coding for alcohol dehydrogenase of Bacillus stearothermophilus and rational shift of the optimum pH." J.Bacteriol.174. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yoshino,K.: "Temperature Sweep Gel Electrophoresis:a simple Method to Detect Point Mutations." Nucleic Acids Res.19. 3153 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 伏見 譲: "「進化分子工学の始まり」(総説)" 生物物理. 32. 22-25 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yomo,T.: "Promenade in the sequence space of bacterial catalase by random mutagenesis." Ann.N.Y.Acad.Sci.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yomo,T.: "No stop codons in the antisense strands of the genes for nylon oligomer degradation." Proc.Natl.Acad.Sci.USA. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kaneko,K.: "Homeochaos:Dynamic Stability of Symbiotic Network with Population Dynamics and Evolving Mutatioin Rates." Physica D.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shirai,T.: "RNase-like domain in DNA-directed RNA polymerase II." Proc.Natl.Acad.Sci.USA. 88. 9056-9060 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Takai,Y.: "Evolutionary Origin of Thyroglobulin by Duplication of Esterase Gene." FEBS Lett.282. 17-22 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Tachibana,H.: "Construction of and 'ATG-TAG vector' and direct expression of chicken lysozyme structural domains in Escherichia coli." Gene. Submitted

    • Related Report
      1991 Annual Research Report
  • [Publications] Yanagawa,H.: "A novel minimum ribozyme with oxidoreduction activity." Biochemistry. 29. 10585-10589 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Yanagawa,H.: "Redox nucleosides in Torula yeast RNA:Isolation and Characterization of 5-hydroxyuridine,8-hydroxyguanosine,and 8-hydroxyadenosine." J.Biol.Chem.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Saitoh,E.: "The human cystation gene family:Cloning of three members and evolutionary relationship between cystatins and Bowman Birk type proteinase inhibitors." Biomed,Biochim.Acta.50. 4-6 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Sugimoto.N.: "Stabilization energy of the catalytic core of the Tetrahymena ribozyme." Chem.Lett.345-348 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Oshima,T.: "Early Biochmical Evolution:Speculations on the Biochemistry of Primitive Life;in Evolution of Life:Fossils,Molecles,and Culture (S.Osawa and T.Honjo,eds.)" Springer Verlag,(Tokyo,Berlin,Heiderberg,New York,London,Paris,Hong-Kong,Barcelna), 460 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nosoh,Y.: "Protein stability and stabilization through protein engineering." Ellis Horwood,London, 223 (1991)

    • Related Report
      1991 Annual Research Report

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Published: 1991-04-01   Modified: 2016-04-21  

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