|Budget Amount *help
¥21,800,000 (Direct Cost : ¥21,800,000)
Fiscal Year 1994 : ¥2,400,000 (Direct Cost : ¥2,400,000)
Fiscal Year 1993 : ¥2,400,000 (Direct Cost : ¥2,400,000)
Fiscal Year 1992 : ¥7,000,000 (Direct Cost : ¥7,000,000)
Fiscal Year 1991 : ¥10,000,000 (Direct Cost : ¥10,000,000)
To furnish significant information to rice molecular genetrics and breeding, we have integrated the conventional linkage map onto the RFLP linkage map. Twenty-four F_2 populations segregating genetic markers and bacterial blight resistance genes were investigated. Fourty-eight genes for the morphological and physiological traits were successfully mapped on RFLP linkage maps as follows : Chromosome 1, fs-1, d-18, d-2, rl-2, spl-6 and eg, Chromosome 2, tri, bl-1, l-chl (t) and spl-2 ; Chromosome 3, chl-1, fc-1, st (t), dl and spl-3 ; Chromosome 4, d-11, Ph, lg, Xa-1 and Xa-2 ; Chromosome 5, gl-1, xa-5, d-1, st (t), spl-7 and nl-1 ; Chromosome 6, dp-1, spl-4 and Cl ; Chromosome 7, g-1, d-6, spl-5 and v-11 ; Chromosome 8, v-8 and sug, Chromosome 9, I-Bf, dp-2, drp-2 and Dn-1 ; Chromosome 10, spl-10 and pgl ; Chromosome 11, Xa-3, Xa-4, Xa-10, z-2 and v-9 ; and Chromosome 12, spl-1 and rl-1 The orientation of the classical linkage map and the RFLP linkage map was completely understood and th
e classical linkage map was a little revised from this study. Some RFLP markers were found to be closely linked to the genes for the morphological and physiological traits, indicating that they could be useful for map-assisted selection in rice breeding program and for map-based cloning of the genes as starting points.
The physical association of the linkage map with chromosomes using cytogenetical variants such as reciprocal translocation, tertiary trisomics and acrotrisomics were also examined. The orientation of linkage groups, the arm location of marker genes and inferred regions of centromeres were decided on chromosomes 1,3,4,5,7,9 and 12.
In addition, recombinant inbred (RI) lines were developed as a permanent mapping population for RFLP/RAPD markers. Using the RI lines, landmark linkage map containing 119 RFLP loci and 26RAPD loci were constructed.
Tertiary trisomics, Acrotrisomics,相互転座,染色体欠失などの染色体変異系統を利用して,その切断点のRFLP地図上での位置を推定した結果,染色体1,3,4,5,7,9,12について染色体と連鎖群の物理的な対応が明らかになった.また,一部はRFLP地図上での動原体の位置あるいはRFLPマーカーの座乗腕が決定された.さらに,染色体地図作成の有力な武器となると期待されるin situ chromosome hybridization およびmicrodisectionを試みた結果,今後のイネ染色体地図作成に使用できる見通しが立った. Less