|Budget Amount *help
¥22,500,000 (Direct Cost : ¥22,500,000)
Fiscal Year 1993 : ¥2,400,000 (Direct Cost : ¥2,400,000)
Fiscal Year 1992 : ¥7,000,000 (Direct Cost : ¥7,000,000)
Fiscal Year 1991 : ¥13,100,000 (Direct Cost : ¥13,100,000)
Immune stimulation of T cells resulted in the conversion CD45 antigen expression form CD45RA 'naive' to CD45RO 'primed' phenotype. It has been suggested that memory T cells reside within CD45RO 'primed' T CELL POOL.As T cells response during EBV-induced infectious mononucleosis is particularly intense. dynamics in the acquisition and generation of T cell memory was evauated largely in this disease model.
1. Monoclonal antibody IMN3.1, prodced with T cells in the acute phase of infectious mononucleosis as imunogen, identified a novel T cell activatin antigen, which was expressed preferentially on apoptosis-prone T cells, such as mononucleosis T cels, thymocytes, and anti-Fas-sensitive T cell lines, but not on resting circulating T cellls.
2. Atypical lymphocytes in infectious mononuchlosis are activated CD45RO^+ T cells. The majority of these stimulated T cells seemed to be eliminated in vivo by apoptotic cel death.
3. CD45RO^+ T cells in infectious mononucleosis expressed Fas as well as IMN3.1 antigen and were sensitive to apoptotsis. In contrast, CD45RO^+ T cells from healthy individuals expressed Fas, but not IMN3.1, antigen, and were resistant to apoptosis. CD45RO^+ blastoid T cells in mononucleosis expressed no detectable leels of bc1-2 protein, whereas CD45RO^+ T cells in healthy subjects were seemingly a quiescent state and expressed considerable levels of bc1-2. The reduced expression of bc1-2 on the majority of stimulated Tcells might render these cells rone to apoptosis. Onyly a few of stimulated T cells expressing both Fas and bcl by unclear mechanisms, however, might be rescued from cell death and might re-enter to a quiescent state as specified functional memory T cells
4. Apoptotic tendency in vitro aging of lymhocytes, monocytes, and neutrohils was in increasing order of sensitivity and seeed to be inversely correlaed with their expression levels of bc1-2.