Analysis of nucleotide sequence of insecticidal crystal protein (ICP) gene which shows highly activity against Lepidoptera insect.
| Project/Area Number |
03454058
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
蚕糸学
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| Research Institution | Hokkaido University |
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Principal Investigator |
IIZUKA Toshihiko Hokkaido Univ., Fac. of Agriculture PROFESSOR, 農学部, 教授 (50001441)
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| Co-Investigator(Kenkyū-buntansha) |
KIKUTA Harunori Hokkaido College of Arts and Sciences ASSOCIATE PROFESSOR, 助教授
ASANO Shinichiro Hokkaido Univ., Fac. of Agriculture ASSISTANT PROFESSOR, 農学部, 助手 (60222585)
BANDO Hisanori Hokkaido Univ., Fac. of Agriculture ASSOCIATE PROFESSOR, 農学部, 助教授 (20189731)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1992: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1991: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | Bacillus thuringiensis / Insecticidal crystal protein / PCR method / Screening / Crystal protein / ICP gene / CryI gene / CryII gene / バチルス・チュ-リンゲンシス / スクリ-ニング / ICP遣伝子 / CryI遣伝子 / CryII遣伝子 |
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| Research Abstract |
A insecticidal crystal protein (ICP) produced by Bacillus thuringiensis (BT), which shows highly activity against the Lepidopteran insect, should be studied from the following two ways:
1. Newly isolation and identification of BT. : Newly BT isolates from soil samples which were collected in the natural conserved area, were identified by routine technique of H-serotype and by newly developed technique depending on agarose gel electrophoresis of cryl genes. After oligo-primers designed by a specific domain were synthesized, these cryI genes were amplified by PCR method with template DNA from BT subspecies. The newly identification technique was very effective in order to clear a high active strain comparing with already known BT strains.
2. Nucleotide sequence analysis of ICP genes. : BT subsp. wuhanensis has been known as high active strain against the silkworm and the cabbage moth. According to a report of Iizuka et al. (1993), subsp. wuhanensis has included cryIA(b), cryIA(c) and cryID genes. In this study, the nucleotide sequence of cryIA(b) was analyzed and compared with another cryIA(b) genes from subspp. thuringiensis berliner 1715 and kurstaki HD-2. The differentiation of amino acid composition between wuhanensis and HD-2 was only one within a active domain. A regulatory mechanisms of each protein expression encoded by three genes was in progressed.
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Report
(3 results)
Research Products
(13 results)
