| Project/Area Number |
03454422
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | THE UNIVERSITY OF TOKUSHIMA |
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Principal Investigator |
HAYASHI Yoshio THE UNIVERSITY OF TOKUSHIMA ORAL PATHOLOGY, PROFESSOR, 歯学部, 教授 (00127854)
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| Co-Investigator(Kenkyū-buntansha) |
DEGUCHI Hiroyo THE UNIVERSITY OF TOKUSHIMA ORAL PATHOLOGY, ASSISTANT, 歯学部, 助手 (80227545)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed (Fiscal Year 1992)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1992: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1991: ¥4,600,000 (Direct Cost: ¥4,600,000)
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| Keywords | Sjogren's syndrome / autoimmune sialadenitis / autoreactive T cells / Cytokine / salivary gland / Sjogren's syndrome / Cytokine / 自己免疫性唾液腺炎 / シェ-グレン症候群 / 疾患モデル / ミュ-タントマウス |
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| Research Abstract |
Sjogren's syndrome in humans is an autoimmune disease characterized by diffuse lymphoid cell infiltrates in the salivary and lacrimal glands, resulting in symptoms of dry mouth and dry eye due to insufficient secretion. Although we have proposed some murine models inducing autoimmune sialadentits, there has not been an established appropriate animal model specific for sjogren' syndrome in humans. We have here established an animal model for Sjogren's syndrome in thymectomezed NFS/sld mutant mice bearing sublingual mucous cell differentiation arrest. Local cytokine gene expression in vivo has been analyzed by direct analysis of RNA obtained from the salivary gland tissues inNFS/sld autoimmune sialadenitis. The expression of cytokine genes was assessed using reverse transcriptase polymerase chain reaction (RT-PCR), in addition to the immunohistochemical analysis. Expression of IL-1 beta, tumor necrosis factor(TNF) was detected before the onset of inflammatory lesions. Furthermore, IL-2, IL-4, and IL-6 mRNA overexpression in the salivary glands was clearly found in accordance with the onset of typical autoimmune sialadenitis, and was upregulated with advancing age. These results indicate that overexpression of these cytokine genes play an important role on the development and progression of organ-localized autoimmunity in the salivary gland in NFS/sld mice.
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