In Vitro Motility Assays of Myosin Blocked with Modifiers
| Project/Area Number | 03454555 |
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
HIRATSUKA Toshiaki Asahikawa Medical College Chemistry Associate Professor, 医学部, 助教授 (30041825)
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| Project Period (FY) |
1991 – 1992
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| Project Status |
Completed(Fiscal Year 1992)
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| Budget Amount *help |
¥5,900,000 (Direct Cost : ¥5,900,000)
Fiscal Year 1992 : ¥1,600,000 (Direct Cost : ¥1,600,000)
Fiscal Year 1991 : ¥4,300,000 (Direct Cost : ¥4,300,000)
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| Keywords | Myosin / Muscle contraction / Motility assays |
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| Research Abstract |
In Vitro Motility Assays of Myosin Blocked with Modifiers
The effects of the chemical modifications of myosin's reactive cysteines on actomyosin adenosine triphosphatase(ATPase) activities and sliding patterns in the in vitro motility assays were examined. The two types of modifications studied were 5-(iodoacetamido)fluorescein labeling of SH1(Cys-707) and 2-(4'- maleimidylanilino)naphthalene-6-sulfonic acid labeling of SH2(Cys-697). Each type of modified myosin inhibited the sliding of actin in motility assays. However, the possibility that the observed inhibition of the sliding of actin induced by modified myosins may result from structural perturbation caused by bulky modifiers cannot be ruled out by the present work.
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Report
(3results)
Research Products
(6results)
